Demonstration of confocal sum frequency microscopy

Locharoenrat, Kitsakorn; Sano, Hikaru; Mizutani, Goro

doi:10.1002/pssc.200879865

Cited by 18 publications

(16 citation statements)

References 13 publications

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“…Using collinearly aligned IR and visible excitation beams, while maintaining a wide-field detection strategy, investigators have achieved a lateral resolution as high as 1.1 μm for SFG imaging in the CH stretching range (94, 95). High axial resolution was furthermore accomplished by illuminating the sample in a wide-field fashion with the IR beam and focusing the visible excitation beam with a high-numerical-aperture lens (96). In this configuration, the visible SFG radiation can be detected through a pinhole with a point detector, and the image is formed by raster scanning the sample with a translation stage.…”

Section: Second-order Vibrational Microscopymentioning

confidence: 99%

Biomolecular Imaging with Coherent Nonlinear Vibrational Microscopy

Chung

Potma

2013

Annu. Rev. Phys. Chem.

105

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Optical imaging with spectroscopic vibrational contrast is a label-free solution for visualizing, identifying, and quantifying a wide range of biomolecular compounds in biological materials. Both linear and nonlinear vibrational microscopy techniques derive their imaging contrast from infrared active or Raman allowed molecular transitions, which provide a rich palette for interrogating chemical and structural details of the sample. Yet nonlinear optical methods, which include both second-order sum-frequency generation (SFG) and third-order coherent Raman scattering (CRS) techniques, offer several improved imaging capabilities over their linear precursors. Nonlinear vibrational microscopy features unprecedented vibrational imaging speeds, provides strategies for higher spatial resolution, and gives access to additional molecular parameters. These advances have turned vibrational microscopy into a premier tool for chemically dissecting live cells and tissues. This review discusses the molecular contrast of SFG and CRS microscopy and highlights several of the advanced imaging capabilities that have impacted biological and biomedical research.

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Section: Second-order Vibrational Microscopymentioning

confidence: 99%

Biomolecular Imaging with Coherent Nonlinear Vibrational Microscopy

Chung

Potma

2013

Annu. Rev. Phys. Chem.

105

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“…As a future potential topic of research we are also interested in studying the transformation of starch in their growth and digestion. Figure 1 shows the setup of the optical sum frequency generation confocal microscope [14]. As a visible light source of wavelength 532 nm (photon energy 2.33 eV) we used a doubled frequency output from a mode-locked cavity-dumped Nd 3+ : YAG laser operating at a repetition rate of 10 Hz.…”

Section: Introductionmentioning

confidence: 99%

Optical Sum Frequency Generation Image of Rice Grains

Li¹,

Miyauchi²,

Tuấn³

et al. 2012

JBNB

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We observed optical sum frequency generation (SFG) images of cross-sections of glutinous rice grains, in order to test a possibility of the SFG microscopy as a tool for monitoring polysaccharide species in rice grains. The SFG response in the CH vibration range was the most intense in the crush cell layer at the edge of the endosperm adjacent to the embryo probably due to optical reflection and scattering effectby the rugged dielectric structure of the crush cell layer. The SFG spectra as a function of the infrared wavelength depended on the measurement position in the endosperm. The SFG results were compared with those by Raman and infrared spectroscopies for the same samples

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“…So far it has been used to image starch and successfully differentiate different saccarides (for details see Mizutani et al 2005;Miyauchi et al 2006;Locharoenrat et al 2008).…”

Section: Sum Frequency Generation Microscopymentioning

confidence: 99%

Optical microscopy in photosynthesis

et al. 2009

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We present a new laser system and nonlinear microscope, designed for differential nonlinear microscopy. The microscope features time-correlated single photon counting of multiphoton fluorescence generated by an alternating pulse-train of orthogonally polarized pulses. The generated nonlinear signal is separated using home-built electronics. Results are presented on fluorescence-detected nonlinear absorption linear anisotropy (FDNALA) of chloroplasts in Asparagus Sprengerii Regel and of Congo Red-stained cellulose.

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Demonstration of confocal sum frequency microscopy

Cited by 18 publications

References 13 publications

Biomolecular Imaging with Coherent Nonlinear Vibrational Microscopy

Biomolecular Imaging with Coherent Nonlinear Vibrational Microscopy

Optical Sum Frequency Generation Image of Rice Grains

Optical microscopy in photosynthesis

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