Demineralized and decellularized bone extracellular matrix-incorporated electrospun nanofibrous scaffold for bone regeneration

Dong, Chanjuan; Qiao, Fangyu; Chen, Guobao; Lv, Yonggang

doi:10.1039/d1tb00895a

Cited by 28 publications

(41 citation statements)

References 58 publications

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“…When the diameter of fibers is reduced to the nanometer range, it endows the electrospun nanofibers with several favorable properties, including high surface area to volume ratio, numerous nano-sized pores and high size uniformity, which highly mimic the structure of the ECM. 11,12 It is known that a nano-structured ECM directly interacts with cells and acts as an active depot that regulates the activity of growth factors. 13 Therefore, the biomimetic features of electrospun nanofibers can play a critical role in facilitating cell growth and guiding tissue regeneration.…”

Section: Introductionmentioning

confidence: 99%

Electrospun nanofibers for bone regeneration: from biomimetic composition, structure to function

et al. 2022

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Section: Introductionmentioning

confidence: 99%

Electrospun nanofibers for bone regeneration: from biomimetic composition, structure to function

et al. 2022

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“…It was believed that the in ammation reaction caused by scaffold should be resolved within 14 days, and chronic in ammation could result in an infection [51] . It has been proven that PCL exhibited good biocompatibility and widely used as a safe biomaterial in BTE led [52,53] . In this study, in order to ensure the biocompatibility of the PCL/dB-ECM nano brous scaffolds, we must rst prepare dB-ECM effectively and safely.…”

Section: Discussionmentioning

confidence: 99%

Poly(ε-caprolactone)-based nanofibrous scaffold incorporated with decellularized bone extracellular matrix as a potential strategy for bone regeneration

Zhang

Zhou

Dong

et al. 2022

Preprint

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Background: Critical size bone defect is still a great challenge in orthopedics. Scaffolds with nanofibrous microstructure seems a promising candidate for critical size bone defect repair. Here we fabricated poly(ε-caprolactone)-based nanofibrous scaffold incorporated with bone derived decellularized extracellular matrix (PCL/dB-ECM) to provide a suitable platform for bone regeneration. Methods: dB-ECM was prepared first and different weight ratios of PCL and dB-ECM was blended to fabricate PCL/dB-ECM nanofibrous scaffolds by electrospinning. The physicochemical properties of the nanofibrous scaffolds were investigated. Rabbit bone mesenchymal stem cells (rBMSCs) were seeded on the nanofibrous scaffolds to evaluate cell proliferation, viability, morphology, cytoskeleton spread and osteogenic differentiation. The ability of the scaffolds to promote bone regeneration in vivo was also assessed by being implanted into a rabbit femoral condyle defect model. Results:The microstructure of the PCL/dB-ECM (2:1) nanofibrous scaffold exhibited randomly arranged nanofibers interlaced to each other to form a network structure. The incorporation of dB-ECM into the scaffold improved the bioactivity of PCL, significantly enhanced the attachment, proliferation and cytoskeleton extension of rBMSCs, as well as remarkably promoted osteogenic differentiation of rBMSCs by elevating the expression of osteogenic-related genes and proteins and by enhancing the ALP activity and calcium deposition. Furthermore, in vivo assays demonstrated that PCL/dB-ECM (2:1) nanofibrous scaffold obviously facilitated new bone formation with better trabecular structures and excellent integration with the surrounding tissues. Conclusion: The PCL/dB-ECM (2:1) nanofibrous scaffold showed excellent bioactivity to facilitate rBMSCs proliferation and osteogenic differentiation in vitro, as well as promoted new bone formation in vivo, suggesting the PCL-based nanofibrous scaffolds incorporated with dB-ECM could be a promising strategy for effective repair of bone defect.

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“…By repopulating cells in these acellular scaffolds, multiple studies have demonstrated the reformation of cell-enriched tissues and their therapeutic potential. Bone-derived ECM scaffolds combined with n-poly (e-caprolactone) nanofibers promoted the attachment, migration, proliferation, and osteogenic differentiation of rat MSCs [ 110 ]. Subsequent transplantation to a rat calvarial critical-size defect model mitigated the foreign body reaction and facilitated bone regeneration.…”

Section: Applications Of Ecm Scaffolds From Other Sources In Tissue E...mentioning

confidence: 99%

Human SMILE-Derived Stromal Lenticule Scaffold for Regenerative Therapy: Review and Perspectives

Santra

Liu

Jhanji

et al. 2022

IJMS

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A transparent cornea is paramount for vision. Corneal opacity is one of the leading causes of blindness. Although conventional corneal transplantation has been successful in recovering patients’ vision, the outcomes are challenged by a global lack of donor tissue availability. Bioengineered corneal tissues are gaining momentum as a new source for corneal wound healing and scar management. Extracellular matrix (ECM)-scaffold-based engineering offers a new perspective on corneal regenerative medicine. Ultrathin stromal laminar tissues obtained from lenticule-based refractive correction procedures, such as SMall Incision Lenticule Extraction (SMILE), are an accessible and novel source of collagen-rich ECM scaffolds with high mechanical strength, biocompatibility, and transparency. After customization (including decellularization), these lenticules can serve as an acellular scaffold niche to repopulate cells, including stromal keratocytes and stem cells, with functional phenotypes. The intrastromal transplantation of these cell/tissue composites can regenerate native-like corneal stromal tissue and restore corneal transparency. This review highlights the current status of ECM-scaffold-based engineering with cells, along with the development of drug and growth factor delivery systems, and elucidates the potential uses of stromal lenticule scaffolds in regenerative therapeutics.

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Demineralized and decellularized bone extracellular matrix-incorporated electrospun nanofibrous scaffold for bone regeneration

Abstract: The extracellular matrix (ECM)-based materials has been employed as scaffolds for bone tissue engineering, providing a suitable microenvironment that possesses biophysical and biochemical cues for cell attachment, proliferation and differentiation....

Cited by 28 publications

References 58 publications

Electrospun nanofibers for bone regeneration: from biomimetic composition, structure to function

Electrospun nanofibers for bone regeneration: from biomimetic composition, structure to function

Poly(ε-caprolactone)-based nanofibrous scaffold incorporated with decellularized bone extracellular matrix as a potential strategy for bone regeneration

Human SMILE-Derived Stromal Lenticule Scaffold for Regenerative Therapy: Review and Perspectives

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