Deletions at intervening sequence splice sites in the alcohol dehydrogenase gene of<i>Drosophila</i>

Benyajati, Cheeptip; Place, Allen R.; Wang, Nancy; Pentz, Ellen S.; Sofer, William

doi:10.1093/nar/10.22.7261

Cited by 71 publications

(34 citation statements)

References 39 publications

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“…Adh fn6 is a null allele (splicing defect) that produces no detectable ADH protein (Benyajati et al 1982). The D2-3 P insertion on the third chromosome served as the source of transposase (Robertson et al 1988).…”

Section: Site-directed Mutagenesismentioning

confidence: 99%

Experimentally Increased Codon Bias in the DrosophilaAdhGene Leads to an Increase in Larval, But Not Adult, Alcohol Dehydrogenase Activity

et al. 2010

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Although most amino acids can be encoded by more than one codon, the synonymous codons are not used with equal frequency. This phenomenon is known as codon bias and appears to be a universal feature of genomes. The translational selection hypothesis posits that the use of optimal codons, which match the most abundant species of isoaccepting tRNAs, results in increased translational efficiency and accuracy. Previous work demonstrated that the experimental reduction of codon bias in the Drosophila alcohol dehydrogenase (Adh) gene led to a significant decrease in ADH protein expression. In this study we performed the converse experiment: we replaced seven suboptimal leucine codons that occur naturally in the Drosophila melanogaster Adh gene with the optimal codon. We then compared the in vivo ADH activities imparted by the wild-type and mutant alleles.

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Section: Site-directed Mutagenesismentioning

confidence: 99%

Experimentally Increased Codon Bias in the DrosophilaAdhGene Leads to an Increase in Larval, But Not Adult, Alcohol Dehydrogenase Activity

et al. 2010

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“…Transcripts from the Adh v gene give rise to three fragments after RNase digestion: one of 320 nucleotides, common to transcripts from the proximal and distal promoters; one of 168 nucleotides, specific to transcripts from the proximal promoter; and one of 134 nucleotides, specific to transcripts from the distal promoter. Transcripts from the endogenous Adh f~6 gene are not spliced properly due to a 4-bp substitution plus 6-bp deletion near the end of the first coding exon and are unstable, present at only 5-10% the steady-state level of wild-type Adh transcripts (Benyajati et al 1982). Transcripts from the distal Adh f~6 promoter yield fragments of 135 and 372 nucleotides; transcripts from the proximal promoter, fragments of 169 and 372 nucleotides.…”

Section: Genes and Development 2193mentioning

confidence: 99%

The role of specific enhancer-promoter interactions in the Drosophila Adh promoter switch.

Corbin¹,

Maniatis²

1989

Genes & Development

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The Drosophila melanogaster alcohol dehydrogenase (Adh) gene is transcribed from two promoters active at different developmental stages. In this paper we show that the promoters are differentially stimulated by two enhancers, the Adh larval enhancer and the Adh adult enhancer. In early larval stages, the larval enhancer stimulates transcription from the proximal promoter; in late larval stages, the two enhancers act synergistically to stimulate transcription from the distal promoter; and in adults, the adult enhancer stimulates transcription from the distal promoter. To determine the basis for these enhancer-promoter interactions, we examined the effect of each enhancer on three different promoters. We found that the adult enhancer is stage specific and stimulates transcription from all three promoters. In contrast, the larval enhancer is potentially active in all stages and stimulates transcription from only two of the three promoters. These observations suggest that normal temporal expression of Adh depends on the stage-specific activity of the adult enhancer and the differential response of the proximal and distal promoters to the larval enhancer.

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“…The magnitude of the deletions compared to the wild type controls used in these studies, coupled with the variable results, makes interpretation of these data difficult. Mutants with smaller deletions that remove splice site consensus sequences have also been studied (41,42 exclusively leads to one of two fates for the mRNA precursor. One fate is that efficient splicing can occur, using alternative splice sites.…”

Section: Discussiqnmentioning

confidence: 99%

The length but not the sequence of the polyoma virus late leader exon is important for both late RNA splicing and stability

Adami¹,

Carmichael

1987

Nucl Acids Res

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ABSTRACT'Polyoma virus late RNA processing provides a convenient model system in which to study the mechanics of splicing in vivo. In order to understand further the role of the untranslated "late leader" unit in late RNA processing we have constructed a group of polyoma viruses with deletions and substitutions in the leader exon. This has allowed us to determine that there is a minimum exon size required for both pre-mRNA splicing and stability in this system. We show here that the non-viability of a mutant (ALM) with a 9 base late leader unit is due to a general defect in late RNA splicing. In addition, ALM-infected cells show at least 40-fold depression in the accumulation of late nuclear RNA (spliced or unspliced). The ALM late promoter, however, functions nearly normally. Substituted leader variants with 51-to 96-base long exons of unrelated sequence are viable (G. Adami and G. Carmichael, J. Virol. 58, 417-425, 1986). We show here that late RNA from one of these substituted leader mutants (containing a 51-base leader exon) is spliced at wild type levels, with virtually no defect in accumulation. Thus, in the polyoma system, splice sites separated by only 9 bases can inhibit each others usage, presumably by steric interference. We suggest that this type of inhibition leads to extreme RNA instability. INTRODUCTION Much work in recent years has been directed towards further understanding the mechanism of splicing of mRNA precursors. Analyses of intron sequence requirements for in vivo mRNA splicing in higher eukaryotes originally indicated the importance of the 5' splice site, including the highly conserved GU dinucleotide at the 5' exon-intron boundary, and the 3' splice site, which consists of a similarly highly conserved dinucleotide, AG, at the 3' boundary, but which additionally includes an upstream stretch of pyrimidines (1-7). More recently, a third site which shows some sequence requirements for splicing in vivo has been identified -the branch point,

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Deletions at intervening sequence splice sites in the alcohol dehydrogenase gene ofDrosophila

Cited by 71 publications

References 39 publications

Experimentally Increased Codon Bias in the DrosophilaAdhGene Leads to an Increase in Larval, But Not Adult, Alcohol Dehydrogenase Activity

Experimentally Increased Codon Bias in the DrosophilaAdhGene Leads to an Increase in Larval, But Not Adult, Alcohol Dehydrogenase Activity

The role of specific enhancer-promoter interactions in the Drosophila Adh promoter switch.

The length but not the sequence of the polyoma virus late leader exon is important for both late RNA splicing and stability

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