Delayed Laboratory Response to COVID-19 Caused by Molecular Diagnostic Contamination

Mögling, Ramona; Meijer, Adam; Berginc, Nataša; Bruisten, Sylvia M.; Charrel, Rémi N.; Coutard, Bruno; Eckerle, Isabella; Enouf, Vincent; Hungnes, Olav; Korukluoğlu, Gülay; Kossyvakis, Thanos; Mentis, Αndreas; Molenkamp, Richard; Muradrasoli, Shaman; Papa, Anna; Pigny, Fiona; Thirion, Laurence; Werf, Sylvie van der; Reusken, Chantal

doi:10.3201/eid2608.201843

Cited by 56 publications

(45 citation statements)

References 2 publications

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“…As previously reported [ 19 ], we identified probable primer contamination using N2 US CDC and E Charité, which prevented us from further evaluating their sensitivity and specificity. Except for these two assays, no false-positive results were observed when testing a wide range of respiratory viruses, a result consistent with previous studies [ 5 , 6 , 10 , 11 , 13 , 14 ].…”

Section: Discussionmentioning

confidence: 99%

Performance Assessment of SARS-CoV-2 PCR Assays Developed by WHO Referral Laboratories

Etievant

Bal

Escuret

et al. 2020

JCM

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A reliable diagnostic assay is crucial to early detect new COVID-19 cases and limit severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) transmission. Since the onset of the COVID-19 pandemic, the World Health Organization has published several diagnostic molecular approaches developed by referral laboratories, including Charité (Germany), HKU (Hong Kong), China CDC (China), US CDC (United States), and Institut Pasteur, Paris (France). We aimed to compare the sensitivity and specificity of these different RT-PCR assays using SARS-CoV-2 cell culture supernatants and clinical respiratory samples. Overall, the different RT-PCR assays performed well for SARS-CoV-2 detection and were all specific except the N Charité (Germany), and N2 US CDC (United States) assays. RdRp Institut Pasteur (IP2, IP4), N China CDC, and N1 US CDC were found to be the most sensitive assays. The data presented herein are of prime importance to facilitate the equipment choice of diagnostic laboratories, as well as for the development of marketed tests.

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Section: Discussionmentioning

confidence: 99%

Performance Assessment of SARS-CoV-2 PCR Assays Developed by WHO Referral Laboratories

Etievant

Bal

Escuret

et al. 2020

JCM

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“…All primers should be ordered with HPLC purification, which ensures the yield and avoids cross contamination from other SARS-CoV-2-related synthesis projects being run on the same equipment at the oligo synthesis facilities (which can lead to false positives). This is particularly a problem during a pandemic where these facilities are handling many oligo synthesis orders focused on the same pathogen14 . It is also recommended that you communicate with the primer synthesis company to inform them that primers are intended for use with a SARS-CoV-2 screening test.…”

mentioning

confidence: 99%

Saliva TwoStep for rapid detection of asymptomatic SARS-CoV-2 carriers

Yang

Meyerson

Clark

et al. 2020

Preprint

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Up to 70% of SARS-CoV-2 infections in working- and school-age people are asymptomatic (Poletti et al., 2020), creating anxiety over reopening workplaces and schools around the world. In the absence of effective treatments or a vaccine, peace of mind will come only with community-based SARS-CoV-2 screening, where many people are tested on a regular basis. However, recent models show that short sample-to-answer turnaround time will be a critical property of effective screening strategies (Larremore et al., 2020). Here, we describe an RT-LAMP test for SARS-CoV-2 in raw saliva that takes about 45 minutes from sample to answer and requires only simple equipment (pipettes and a heating source). The assay has a limit of detection of 100 virions per microliter, and targets two separate regions of the SARS-CoV-2 genome. By combining rapid sample-to-answer turnaround time with the use of saliva, our RT-LAMP assay provides a low-complexity, portable, and robust system for real-time community screening.

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“…The addition to the EVA catalog of lyophilized primers, probes, and positive controls for the molecular detection of COVID-19 has played a major role to help laboratories in Europe prepare for detection of cases. A large part of the delay in establishing diagnostic capacities can be attributed to multiple contamination incidents at the production sites of industrial distributors of primers and probes, 18 and this EVA product was a timely solution to that problem. Especially at these early stages of the viral emergence, giving access to reference material is mandatory as there is no economic market meeting these needs.…”

Section: Discussionmentioning

confidence: 99%

The Importance of Biobanking for Response to Pandemics Caused by Emerging Viruses: The European Virus Archive As an Observatory of the Global Response to the Zika Virus and COVID-19 Crisis

Lamballerie¹,

Rus²,

Korva³

et al. 2020

Biopreservation and Biobanking

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Delayed Laboratory Response to COVID-19 Caused by Molecular Diagnostic Contamination

Cited by 56 publications

References 2 publications

Performance Assessment of SARS-CoV-2 PCR Assays Developed by WHO Referral Laboratories

Performance Assessment of SARS-CoV-2 PCR Assays Developed by WHO Referral Laboratories

Saliva TwoStep for rapid detection of asymptomatic SARS-CoV-2 carriers

The Importance of Biobanking for Response to Pandemics Caused by Emerging Viruses: The European Virus Archive As an Observatory of the Global Response to the Zika Virus and COVID-19 Crisis

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