Delayed blastulation, multinucleation, and expansion grade are independently associated with live-birth rates in frozen blastocyst transfer cycles

Desai, Nina; Ploskonka, Stephanie; Goodman, Linnea R.; Attaran, Marjan; Goldberg, Jeffrey M.; Austin, C. R.; Falcone, Tommaso

doi:10.1016/j.fertnstert.2016.07.1095

Cited by 107 publications

(83 citation statements)

References 38 publications

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“…The final inclusion was 13 studies that met the selection criteria for our meta‐analysis. Hence, 23 studies were included in the following meta‐analyses . The main characteristics and assessment for risk of bias of the included studies are presented in Table .…”

Section: Resultsmentioning

confidence: 99%

“…Hence, 23 studies were included in the following meta-analyses. 4,6,7,[11][12][13][14][15][16][17][18][19][20][21][22][23][24][25][26][27][28][29][30] The main characteristics and assessment for risk of bias of the included studies are presented in Table 1.…”

Section: Results Of the Searchesmentioning

confidence: 99%

“…To overcome the endometrial factor, a number of studies compared the pregnancy outcomes between day 5 and day 6 BET following vitrified‐warmed cycles. However, the pregnancy outcomes between day 5 and day 6 BET following vitrified‐warmed cycle have previously yielded conflicting results …”

Section: Introductionmentioning

confidence: 99%

“…However, the pregnancy outcomes between day 5 and day 6 BET following vitrified-warmed cycle have previously yielded conflicting results. 6,7 We therefore conducted a meta-analysis of available literature to compare the pregnancy outcomes after day 5 versus day 6 BET following vitrifiedwarmed cycle.…”

Section: Introductionmentioning

confidence: 99%

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Pregnancy outcomes after day 5 versus day 6 blastocyst‐stage embryo transfer: A systematic review and meta‐analysis

Wang²,

Sun

et al. 2020

J of Obstet and Gynaecol

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Aim To compare the pregnancy outcomes after day 5 blastocyst‐stage embryo transfers (BET) versus day 6 BET following vitrified‐warmed cycle and to evaluate whether the number of embryos transferred and the chromosomal status of embryo influence effect estimates. Methods A literature search (PubMed, Embase and MEDLINE) up to January 2019 was conducted to identify studies where women with day 6 BET were compared to women with day 5 BET. Only studies published in English language, on peer‐reviewed journal were considered eligible. The following subgroup analyses were performed: (i) number of embryos transferred and (ii) chromosomal status of embryo. Results From a total of 1956 articles identified, 23 observational studies were included in the meta‐analysis. We observed that day 6 BET were associated with lower implantation rate (risk ratio, RR: 1.17, 95% confidence interval, CI: 1.10–1.24), clinical pregnancy rate (RR: 1.17, 95% CI: 1.10–1.24), ongoing pregnancy rate (RR: 1.15, 95% CI: 1.07–1.24) and live birth rate (RR: 1.22, 95% CI: 1.11–1.33) than day 5 BET following vitrified‐warmed cycle. The subgroup analysis found that the superiority of day 5 BET compared with day 6 BET is influenced by the number of embryos transferred and chromosomal status of embryos. Conclusion Current evidence shows that day 5 BET is superior to day 6 BET following vitrified‐warmed cycle in clinical practice. Due to the overall low quality of available evidence, more larger and well‐conducted studies are needed to compare the pregnancy outcomes between day 5 and day 6 BET before drawing a clear conclusion.

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Section: Resultsmentioning

confidence: 99%

Section: Results Of the Searchesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Pregnancy outcomes after day 5 versus day 6 blastocyst‐stage embryo transfer: A systematic review and meta‐analysis

Wang²,

Sun

et al. 2020

J of Obstet and Gynaecol

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“…The three morphological defects, which we evaluated by TLS, were not different between positive and negative chemical and clinical pregnancies and live birth. In contrast, other studies reported the negative impact of DC as well as MN on implantation rate (Desai et al, ; Goodman et al, ) and birth outcome (Desai et al, ; Desch et al, ).…”

Section: Discussionmentioning

confidence: 86%

Assessing ICSI outcome by combining non‐invasive indicators: Early time‐lapse morphokinetics and apoptosis in associated cumulus cells among women with the polycystic ovarian syndrome

Tabibnejad

Aflatoonian

Motamedzadeh

et al. 2018

Molecular Reproduction Devel

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Cumulus cells features and embryo developmental events can be considered as noninvasive indicators for embryo selection and clinical outcomes. A combination of time-lapse morphokinetic parameters and cumulus cell apoptosis in women with polycystic ovarian syndrome (PCOS) was evaluated for predicting pregnancy outcome. We assessed a total of 547 embryos from 100 intracytoplasmic sperm injection (ICSI) cycles. Time-lapse records were interpreted in time to pronuclear fading (tPNf), time to 2 to 8 cells (t2-t8), direct cleavage, reverse cleavage, and also for the presence of multinucleation. Percentages of apoptosis were identified in 100 associated cumulus cell samples using the TDT-mediated dUTP-biotin nick end-labeling assay. The significant decrease of apoptotic cumulus cells was detected in patients with chemical and clinical pregnancies as well as live birth among patients PCOS and in the tubal infertility group (p > 0.05). Furthermore, significantly higher implantation rate and also significantly lower cases of early pregnancy loss were observed in the group of oocytes with less apoptotic cumulus cells. Multivariate logistic regression analysis showed that tPNf together with cumulus cell apoptosis were independent prognostic factors of chemical pregnancy, clinical pregnancy rate, and live birth. Time-lapse embryo parameters may not reflect the cumulus cell apoptosis rate. However, the rate of apoptotic cumulus cells is significantly associated with ICSI outcome using Day 3 embryo transfer.

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Selection of high‐quality and viable blastocysts based on timing of morula compaction and blastocyst formation

Harada

Maeda

Fukunaga

et al. 2019

Reprod Medicine & Biology

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PurposeThe time‐lapse system is a device that allows continuous monitoring without removing embryos from the incubator. Using a time‐lapse system, we retrospectively investigated cleavage speed time points as potential indicators for selecting high‐quality viable blastocysts.MethodsThis study included 963 zygotes of two pronuclei retrieved from 196 patients between January 2015 and December 2016. All embryos in culture were monitored by time‐lapse after intracytoplasmic sperm injection. Of 492 blastocysts developed in vitro, 128 vitrified‐warmed single blastocyst transfers were classified into pregnancy and non‐pregnancy groups, and the parameters were compared.ResultsIn the pregnancy group, timing of both morula compaction and regular blastocyst formation was significantly faster than in the non‐pregnancy group. Furthermore, the optimal cutoff values for compacted morula (94.9 hours) and regular blastocyst (113.9 hours) were determined using the receiver operator characteristic curve analysis. Embryos that formed compacted morulae within 94.9 hours and developed into regular blastocysts within 113.9 hours were associated with a significantly higher pregnancy rate than those that did not (44.4% vs 16.0%).ConclusionThe timing of morula compaction and regular blastocyst formation is important as an indicator of high‐quality blastocysts to increase odds for pregnancy after embryo transfer.

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Delayed blastulation, multinucleation, and expansion grade are independently associated with live-birth rates in frozen blastocyst transfer cycles

Cited by 107 publications

References 38 publications

Pregnancy outcomes after day 5 versus day 6 blastocyst‐stage embryo transfer: A systematic review and meta‐analysis

Pregnancy outcomes after day 5 versus day 6 blastocyst‐stage embryo transfer: A systematic review and meta‐analysis

Assessing ICSI outcome by combining non‐invasive indicators: Early time‐lapse morphokinetics and apoptosis in associated cumulus cells among women with the polycystic ovarian syndrome

Selection of high‐quality and viable blastocysts based on timing of morula compaction and blastocyst formation

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