Dehydration stress responses of yeasts Torulaspora delbrueckii, Metschnikowia pulcherrima and Lachancea thermotolerans: Effects of glutathione and trehalose biosynthesis

Câmara, Antonio de Anchieta; Marechal, Pierre-André; Tourdot-Maréchal, Raphaëlle; Husson, Florence

doi:10.1016/j.fm.2018.12.008

Cited by 28 publications

(20 citation statements)

References 61 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Ward et al [59] showed that the commercially available yeast contained Se compounds combined with glutathione, which corroborated the earlier findings of Arnaudquilhem et al [21]. According to literature data [37], cell protection against oxidative processes predominantly occurs through the functioning of glutathione as a reducing agent. The antioxidant effect of this process may involve protecting the -SH groups against irreversible oxidation and thus against irreversible loss of biological activity of many enzymes [25].…”

Section: Se Speciationsupporting

confidence: 68%

“…Glycogen is an intracellular polymer composed of glucose subunits, thus affects the normal growth of yeast by providing constant access to a carbon source [40]. It was found that, in yeast cells, Trehalose protects lipids and proteins against oxidation by changing the activity of individual biochemical reactions, which has an inhibitory effect on the generation of ROS [37]. According to Saharan and Sharma [33], trehalose functions in coordination with glutathione to control stress associated with free radicals in cells.…”

Section: Effect Of Se On Trehalose and Glycogen Contentmentioning

confidence: 99%

See 1 more Smart Citation

Metabolic Response of the Yeast Candida utilis During Enrichment in Selenium

Kieliszek

Bierła

Jiménez-Lamana

et al. 2020

IJMS

View full text Add to dashboard Cite

Selenium (Se) was found to inhibit the growth of the yeast Candida utilis ATCC 9950. Cells cultured in 30 mg selenite/L supplemented medium could bind 1368 µg Se/g of dry weight in their structures. Increased accumulation of trehalose and glycogen was observed, which indicated cell response to stress conditions. The activity of antioxidative enzymes (glutathione peroxidase, glutathione reductase, thioredoxin reductase, and glutathione S-transferase) was significantly higher than that of the control without Se addition. Most Se was bound to water-insoluble protein fraction; in addition, the yeast produced 20–30 nm Se nanoparticles (SeNPs). Part of Se was metabolized to selenomethionine (10%) and selenocysteine (20%). The HPLC-ESI-Orbitrap MS analysis showed the presence of five Se compounds combined with glutathione in the yeast. The obtained results form the basis for further research on the mechanisms of Se metabolism in yeast cells.

show abstract

Section: Se Speciationsupporting

confidence: 68%

Section: Effect Of Se On Trehalose and Glycogen Contentmentioning

confidence: 99%

Metabolic Response of the Yeast Candida utilis During Enrichment in Selenium

Kieliszek

Bierła

Jiménez-Lamana

et al. 2020

IJMS

View full text Add to dashboard Cite

show abstract

“…The results obtained will help to better understand certain physiological responses of non-Saccharomyces yeasts to dehydration, leading to the promotion and production of new high performance dehydrated non-Saccharomyces yeasts strains to be used in food and beverages elaboration. According to the mentioned work [49], yeasts grown in nutrient-rich medium accumulated glutathione leading to a higher resistance to dehydration, whilst the nutrient-poor medium induced the cells to accumulate large amounts of trehalose, which partially protected them from GSSG accumulation.…”

Section: Mechanisms Of Microbial Resistance To Environment Changes Thmentioning

confidence: 98%

“…Câmara et al [49] studied the effects of glutathione and trehalose biosynthesis in the dehydration stress responses of three non-Saccharomyces yeasts strains (Torulaspora delbrueckii CBS4865, Metschnikowia pulcherrima CBS5833 and Lachancea thermotolerans CBS6340). The results obtained will help to better understand certain physiological responses of non-Saccharomyces yeasts to dehydration, leading to the promotion and production of new high performance dehydrated non-Saccharomyces yeasts strains to be used in food and beverages elaboration.…”

Section: Mechanisms Of Microbial Resistance To Environment Changes Thmentioning

confidence: 99%

The Importance of Yeasts on Fermentation Quality and Human Health-Promoting Compounds

Vilela

2019

Fermentation

View full text Add to dashboard Cite

Non-Saccharomyces are important during wine fermentation once they influence wine composition. In the early stages of wine fermentation, and together with indigenous or commercial strains of Saccharomyces cerevisiae, non-Saccharomyces are able to transform grape-must sugars into ethanol, CO2, and other important secondary metabolites. A better understanding of yeast biochemistry will allow the selection of yeast strains that have defined specific influences on fermentation efficiency, wine quality, and the production of human health-promoting compounds. Yeast metabolism produces compounds derived from tryptophan, melatonin, and serotonin, which are found in fermented beverages, such as wine and beer. Melatonin is a neurohormone secreted from the pineal gland and has a wide-ranging regulatory and neuroprotective role, while serotonin, as well as being a precursor of melatonin synthesis, is also a neurotransmitter. This review summarizes the importance of some conventional and nonconventional yeast strains’ alcoholic fermentations, especially in the production of metabolites that promote human health and thus, attract consumers attention towards fermented beverages. A brief reference is also made on fermented beverages containing probiotics, namely kombucha, also known as kombucha tea, and its interesting microorganism’s symbiotic relationships named SCOBY.

show abstract

“…Many studies performed in this area have revealed a number of earlier unknown protective reactions and various protective compounds that help cells to survive in such extreme conditions. These protective compounds include trehalose, polyols, proline, glutathione, heat shock proteins, and others [18][19][20][21][22][23][24][25][26][27][28][29][30][31][32][33]. It was also shown that all intracellular organelles are subject to definite changes during dehydration and rehydration procedures [8].…”

Section: Introductionmentioning

confidence: 99%

Anhydrobiosis in Yeasts: Changes in Mitochondrial Membranes Improve the Resistance of Saccharomyces cerevisiae Cells to Dehydration–Rehydration

Khroustalyova

Rapoport

2019

Fermentation

View full text Add to dashboard Cite

Anhydrobiosis is a unique state of live organisms in which their metabolism is temporary reversibly suspended as the result of strong dehydration of their cells. This state is widely used currently during large-capacity production of active dry baker's yeast. Other strains of the yeast Saccharomyces cerevisiae, as well as other yeast species that could potentially find use in modern biotechnology, are not resistant to dehydration-rehydration treatments. To improve their resistance, the main factors that influence cell survival during such treatment need to be revealed. This study showed the importance of mitochondria for yeast cell survival during transfer into anhydrobiosis, a factor that was strongly underestimated until this study. It was revealed that the external introduction inside yeast cells of 50 µM of lithocholic acid (LCA), an agent that induces changes in glycerophospholipids in mitochondrial membranes, in combination with 1% DMSO, may improve the survival rate of dehydrated cells. The influence of LCA upon yeast cell resistance to dehydrationrehydration was not linked with changes in the state of the cells' plasma membrane.

show abstract

Dehydration stress responses of yeasts Torulaspora delbrueckii, Metschnikowia pulcherrima and Lachancea thermotolerans: Effects of glutathione and trehalose biosynthesis

Cited by 28 publications

References 61 publications

Metabolic Response of the Yeast Candida utilis During Enrichment in Selenium

Metabolic Response of the Yeast Candida utilis During Enrichment in Selenium

The Importance of Yeasts on Fermentation Quality and Human Health-Promoting Compounds

Anhydrobiosis in Yeasts: Changes in Mitochondrial Membranes Improve the Resistance of Saccharomyces cerevisiae Cells to Dehydration–Rehydration

Contact Info

Product

Resources

About