Dehydrated aqueous two-phase system micro-domains retain their shape upon rehydration to allow patterned reagent delivery to cells

Bathany, Cédric; Park, Juhee; Cho, Yoon‐Kyoung; Takayama, Shuichi

doi:10.1039/c3tb21004a

Cited by 8 publications

(10 citation statements)

References 38 publications

(42 reference statements)

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“…Aqueous two-phase systems have recently been reported in the context of cell culture in a microfluidic environment. Takayama and colleagues described a patterning technique for selective cell and reagent deposition, 23,24 whereas Petrak et al microprinted cells to create organized constructs for tissue engineering applications. 25 The aqueous two-phase technique is used here for the first time to create flow focused microwounds in a confluent cell layer.…”

Section: Introductionmentioning

confidence: 99%

The potential of microfluidic lung epithelial wounding: towardsin vivo-like alveolar microinjuries

Felder

Stucki

et al. 2014

Integr. Biol.

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Idiopathic pulmonary fibrosis (IPF) remains a major clinical challenge to date. Repeated alveolar epithelial microinjuries are considered as the starting point and the key event in both the development and the progression of IPF. Various pro-fibrotic agents have been identified and shown to cause alveolar damage. In IPF, however, no leading cause of alveolar epithelial microinjuries can be identified and the exact etiology remains elusive. New results from epidemiologic studies suggest a causal relation between IPF and frequent episodes of gastric refluxes resulting in gastric microaspirations into the lung. The effect of gastric contents on the alveolar epithelium has not been investigated in detail. Here, we present a microfluidic lung epithelial wounding system that allows for the selective exposure of alveolar epithelial cells to gastric contents. The system is revealed to be robust and highly reproducible. The thereby created epithelial microwounds are of tiny dimensions and best possibly reproduce alveolar damage in the lung. We further demonstrate that exposure to gastric contents, namely hydrochloric acid (HCl) and pepsin, directly damages the alveolar epithelium. Together, this novel in vitro wounding system allows for the creation of in vivo-like alveolar microinjuries with the potential to study lung injury and alveolar wound repair in vitro.

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Section: Introductionmentioning

confidence: 99%

The potential of microfluidic lung epithelial wounding: towardsin vivo-like alveolar microinjuries

Felder

Stucki

et al. 2014

Integr. Biol.

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“…[1,2,7,8,12,15,16,[23][24][25][26][27] The surrounding matrices that help to support the printed arbitrary 3D architectures include viscous oils, elastomers, self-healing hydrogels, and granular gel suspensions. [1,2,7,8,12,15,16,[23][24][25][26][27] The surrounding matrices that help to support the printed arbitrary 3D architectures include viscous oils, elastomers, self-healing hydrogels, and granular gel suspensions.…”

Section: Doi: 101002/adma201904631mentioning

confidence: 99%

“…[6,26,[31][32][33][34][35][36][37][38] An ATPS is a versatile phase-separated system consisting of different hydrophilic polymers, such as poly(ethylene oxide) (PEO) and dextran (DEX). [6,26,[31][32][33][34][35][36][37][38] An ATPS is a versatile phase-separated system consisting of different hydrophilic polymers, such as poly(ethylene oxide) (PEO) and dextran (DEX).…”

Section: Doi: 101002/adma201904631mentioning

confidence: 99%

“…To address this issue, embedded 3D printing techniques where liquid inks are printed within a surrounding matrix have emerged. [1,2,7,8,12,15,16,[23][24][25][26][27] The surrounding matrices that help to support the printed arbitrary 3D architectures include viscous oils, elastomers, self-healing hydrogels, and granular gel suspensions. [1,2,4,23,28] Despite impressive developments, achieving a balance between the geometric, mechanical, and biochemical functionality of printed constructs remains intricately difficult.…”

Section: Doi: 101002/adma201904631mentioning

confidence: 99%

“…The cyto-mimetic aqueous two-phase system (ATPS) presents a unique platform for tissue constructs via embedded 3D bioprinting. [6,26,[31][32][33][34][35][36][37][38] An ATPS is a versatile phase-separated system consisting of different hydrophilic polymers, such as poly(ethylene oxide) (PEO) and dextran (DEX). The interfacial tension can be three orders of magnitude lower than that of typical aqueous/ organic phases; [39] the low interfacial tension is beneficial for suppressing the deformation of printed liquid structures.…”

Section: Wwwadvmatde Wwwadvancedsciencenewscommentioning

confidence: 99%

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Freeform, Reconfigurable Embedded Printing of All‐Aqueous 3D Architectures

Luo

Yuan

et al. 2019

Advanced Materials

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Aqueous microstructures are challenging to create, handle, and preserve since their surfaces tend to shrink into spherical shapes with minimum surface areas. The creation of freeform aqueous architectures will significantly advance the bioprinting of complex tissue‐like constructs, such as arteries, urinary catheters, and tracheae. The generation of complex, freeform, three‐dimensional (3D) all‐liquid architectures using formulated aqueous two‐phase systems (ATPSs) is demonstrated. These all‐liquid microconstructs are formed by printing aqueous bioinks in an immiscible aqueous environment, which functions as a biocompatible support and pregel solution. By exploiting the hydrogen bonding interaction between polymers in ATPS, the printed aqueous‐in‐aqueous reconfigurable 3D architectures can be stabilized for weeks by the noncovalent membrane at the interface. Different cells can be separately combined with compartmentalized bioinks and matrices to obtain tailor‐designed microconstructs with perfusable vascular networks. The freeform, reconfigurable embedded printing of all‐liquid architectures by ATPSs offers unique opportunities and powerful tools since limitless formulations can be designed from among a breadth of natural and synthetic hydrophilic polymers to mimic tissues. This printing approach may be useful to engineer biomimetic, dynamic tissue‐like constructs for potential applications in drug screening, in vitro tissue models, and regenerative medicine.

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Aqueous Two-Phase Systems for Micropatterning of Cells and Biomolecules

Ham

Tavana

2018

Open-Space Microfluidics: Concepts, Implementations, Applications

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Dehydrated aqueous two-phase system micro-domains retain their shape upon rehydration to allow patterned reagent delivery to cells

Cited by 8 publications

References 38 publications

The potential of microfluidic lung epithelial wounding: towardsin vivo-like alveolar microinjuries

The potential of microfluidic lung epithelial wounding: towardsin vivo-like alveolar microinjuries

Freeform, Reconfigurable Embedded Printing of All‐Aqueous 3D Architectures

Aqueous Two-Phase Systems for Micropatterning of Cells and Biomolecules

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