Degrader density determines spatial variability of 2,6-dichlorobenzamide mineralisation in soil

Sjøholm, Ole Rüdiger; Aamand, Jens; Sørensen, Jan; Nybroe, Ole

doi:10.1016/j.envpol.2009.07.002

Cited by 24 publications

(13 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The filter standards were frozen in liquid nitrogen and freeze-dried before extraction of DNA (Section 2.4). For the Aminobacter assay, the primers Aminob151F (5 0 -ACCGTATACGTCCGATAGGA-3 0 ) (Sjøholm et al, 2010) and Aminob597R (TCCACTCACCTCTCTCG-GAC-3 0 ) (this study) were used, amplifying a 463 bp PCR product. For the bbdA assay, the primers bbdA-F (5 0 -ATATCACGGCCGGTAC-TATGCCAA-3 0 ) and bbdA-R (5 0 -TCTTCCAAGATCGAACAACCCGGA-3 0 ) (T'Syen et al, unpublished data) were used, amplifying a 156 bp PCR product.…”

Section: Quantification Of Msh1 Presence and Activitymentioning

confidence: 99%

Degradation of trace concentrations of the persistent groundwater pollutant 2,6-dichlorobenzamide (BAM) in bioaugmented rapid sand filters

et al. 2015

Self Cite

View full text Add to dashboard Cite

Section: Quantification Of Msh1 Presence and Activitymentioning

confidence: 99%

Degradation of trace concentrations of the persistent groundwater pollutant 2,6-dichlorobenzamide (BAM) in bioaugmented rapid sand filters

et al. 2015

Self Cite

View full text Add to dashboard Cite

“…Thawed sand (0.5 g) was extracted using the PowerLyzer PowerSoil DNA Isolation kit (MO BIO, Carlsbad, CA). Quantitative PCR (qPCR) on 1-μL subsamples was performed with Aminobacter-specific primers (Sjøholm et al 2010a) in a total volume of 20 μL, containing 2 μL BSA, 0.8 μL of each of primers Aminob 151F (ACC GTA TAC GTC CGA TAG GA) and Aminob 585R (CTC GGA CTC TAB ATT GCC AF), 10 μL SiBir SYBR Green (Bioron, Ludwigshafen, Germany), and 5.4 μL water on an iCycler iQ (Bio-Rad, Hempstead, UK). Dilutions of DNA extracted from 0.5 g sand added with 100 μL MSH1 culture (4×10 9 cells/mL) were used as standards.…”

Section: Degradation Of Bammentioning

confidence: 99%

Using 2,6-dichlorobenzamide (BAM) degrading Aminobacter sp. MSH1 in flow through biofilters—initial adhesion and BAM degradation potentials

Albers

Jacobsen

Aamand

2013

Appl Microbiol Biotechnol

Self Cite

View full text Add to dashboard Cite

Micropollutants in groundwater are given significant attention by water companies and authorities due to an increasing awareness that they might be present even above the legal threshold values. As part of our investigations of the possibility to remove the common groundwater pollutant 2,6-dichlorobenzamide (BAM) by introducing the efficient BAM degrader Aminobacter sp. MSH1 into biologically active sand filters, we investigated if the strain adheres to filters containing various filter materials and if the initial adherence and subsequent degradation of BAM could be optimized. We found that most of the inoculated MSH1 cells adhered fast and that parameters like pH and ionic strength had only a minor influence on the adhesion despite huge influence on cell surface hydrophobicity. At the given growth protocol, the MSH1 strain apparently developed a subpopulation that had lost its ability to adhere to the filter materials, which was supported by attempted reinoculation of non-adhered cells. Analysis by quantitative PCR showed that most cells adhered in the top of the filters and that some of these were lost from the filters during initial operation, while insignificant losses occurred after 1 day of operation. The inoculated filters were found to degrade 2.7 μg/L BAM to below 0.1 μg/L at a 1.1-h residence time with insignificant formation of known degradation products. In conclusion, most filter materials and water types should be feasible for inoculation with the MSH1 strain, while more research into degradation at low concentrations and temperatures is needed before this technology is ready for use at actual waterworks.

show abstract

“…The two above-mentioned BAMdegrading Aminobacter strains were originally isolated from BAM-contaminated soils, and both are able to mineralise BAM at low concentrations relevant for contaminated water resources ). The Aminobacter genus has also been shown to play an important role for the in situ mineralisation of BAM (Sjøholm et al 2010). The strains are therefore interesting candidates for bioremediation of BAM-contaminated drinking water resources.…”

Section: Introductionmentioning

confidence: 99%

Intermediate accumulation of metabolites results in a bottleneck for mineralisation of the herbicide metabolite 2,6-dichlorobenzamide (BAM) by Aminobacter spp.

Simonsen

Badawi

Anskjær

et al. 2011

Appl Microbiol Biotechnol

Self Cite

View full text Add to dashboard Cite

Degradation and mineralisation of the groundwater contaminant 2,6-dichloro-benzamide (BAM) was investigated in two Aminobacter strains focussing on the induction of BAM degradation and mineralisation and occurrence of intermediate metabolites. The BAM degradation rate was independent of whether the cells were pre-grown in the absence or presence of BAM, thus indicating that the first step in the degradation pathway was constitutively expressed. In contrast, (14)CO(2) production was stimulated when cells were pre-grown in the presence of BAM, suggesting that one or more of the subsequent steps in the degradation pathway were inducible. Accumulation of 2,6-dichlorobenzoate (DCBA) during degradation of BAM demonstrated that the first step involved amidase activity. Mass balance calculations and thin-layer chromatography coupled with autoradiographic detection indicated that degradation of DCBA and at least one unknown metabolite may comprise a bottleneck for BAM mineralisation by Aminobacter spp. The study thus provides novel information about the BAM degradation pathway and points to the involvement of unknown intermediate metabolites in degradation of this important groundwater contaminant.

show abstract

Degrader density determines spatial variability of 2,6-dichlorobenzamide mineralisation in soil

Cited by 24 publications

References 16 publications

Degradation of trace concentrations of the persistent groundwater pollutant 2,6-dichlorobenzamide (BAM) in bioaugmented rapid sand filters

Degradation of trace concentrations of the persistent groundwater pollutant 2,6-dichlorobenzamide (BAM) in bioaugmented rapid sand filters

Using 2,6-dichlorobenzamide (BAM) degrading Aminobacter sp. MSH1 in flow through biofilters—initial adhesion and BAM degradation potentials

Intermediate accumulation of metabolites results in a bottleneck for mineralisation of the herbicide metabolite 2,6-dichlorobenzamide (BAM) by Aminobacter spp.

Contact Info

Product

Resources

About