Degradation of Swainsonine by the NADP-Dependent Alcohol Dehydrogenase A1R6C3 in Arthrobacter sp. HW08

Abstract

Swainsonine is an indolizidine alkaloid that has been found in locoweeds and some fungi. Our previous study demonstrated that Arthrobacter sp. HW08 or its crude enzyme extract could degrade swainsonie efficiently. However, the mechanism of swainsonine degradation in bacteria remains unclear. In this study, we used label-free quantitative proteomics method based on liquid chromatography-electrospray ionization-tandem mass spectrometry to dissect the mechanism of swainsonine biodegradation by Arthrobacter sp. HW08. The results showed that 129 differentially expressed proteins were relevant to swainsonine degradation. These differentially expressed proteins were mostly related to the biological process of metabolism and the molecular function of catalytic activity. Among the 129 differentially expressed proteins, putative sugar phosphate isomerase/epimerase A1R5X7, Acetyl-CoA acetyltransferase A0JZ95, and nicotinamide adenine dinucleotide phosphate (NADP)-dependent alcohol dehydrogenase A1R6C3 were found to contribute to the swainsonine degradation. Notably, NADP-dependent alcohol dehyrodgenase A1R6C3 appeared to play a major role in degrading swainsonine, but not as much as Arthrobacter sp. HW08 did. Collectively, our findings here provide insights to understand the mechanism of swainsonine degradation in bacteria.

Role of the luxS gene in bacteriocin biosynthesis by Lactobacillus plantarum KLDS1.0391: A proteomic analysis

“…The peptide mixture of each sample was separated on a high-performance liquid chromatography (HPLC) system (EASY-nLC 1000, Thermo Finnigan, San Jose, CA, USA). After HPLC separation, the peptides from all replicates were analysed using a Q-Exactive MS (Thermo Finnigan) for 120 min 40 , 41 . Notably, each sample was processed three times, and the MS experiments for each sample were performed in triplicate to avoid contingency of the date and assure data reliability.…”

Effects of Ensiling Oxytropis glabra with Whole-Plant Corn at Different Proportions on Fermentation Quality, Alkaloid Swainsonine Content, and Lactic Acid Bacteria Populations

“…The 1,2-di-hydroxyl structure of SW is relatively unstable and may be oxidized by alcohol dehydrogenases [45]. Nicotinamide adenine dinucleotide phosphate (NADP)-dependent alcohol dehydrogenase has a strong ability to degrade SW [46], and this enzyme is produced by some LAB species, such as Leuconostoc cremoris [47]. In this study, the SW removal capability of LAB belonging to different species in fresh materials and silages has been explored preliminarily.…”