Degradation of noncollagenous components by neutrophil elastase reduces the mechanical strength of rat periodontal ligament

Ujiie, Yuko; Shimada, Akemi; Komatsu, Koichiro; Gomi, Kazuhiro; Oida, Shinichiro; Arai, Takashi; Fukae, Makoto

doi:10.1111/j.1600-0765.2007.00990.x

Cited by 21 publications

(17 citation statements)

References 42 publications

(66 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…20,21,22 An increase in neutrophil elastase activity increases fibronectin degradation fragments and decreases periodontal mechanical strength. 23 In addition, clinical treatments such as scaling, root planning and curettage weaken elastase activity and restore cFn level. 24 …”

Section: Discussionmentioning

confidence: 99%

Expression of cellular fibronectin mRNA in adult periodontitis and peri-implantitis: a real-time polymerase chain reaction study

Cao

Kang

et al. 2013

Int J Oral Sci

View full text Add to dashboard Cite

Cellular fibronectin (cFn) is a type of bioactive non-collagen glycoprotein regarded as the main substance used to maintain periodontal attachment. The content of cFn in some specific sites can reflect the progress of periodontitis or peri-implantitis. This study aims to evaluate the expression of cFn messenger RNA (mRNA) in tissues of adult periodontitis and peri-implantitis by real-time fluorescent quantitative polymerase chain reaction (PCR) and to determine its clinical significance. A total of 30 patients were divided into three groups of 10: healthy, adult periodontitis and peri-implantitis. Periodontal tissue biopsies (1 mm×1 mm×1 mm) from each patient were frozen in liquid nitrogen. Total RNA was extracted from these tissues, and the content, purity and integrity were detected. Specific primers were designed according to the sequence, and the mRNA expression levels of cellular fibronectin were detected by real-time PCR. The purity and integrity of the extracted total RNA were both high, and the specificity of amplified genes was very high with no other pollution. The mRNA expression of cFn in the adult periodontitis group (1.526±0.441) was lower than that in the healthy group (3.253±0.736). However, the mRNA expression of cFn in the peri-implantitis group (3.965±0.537) was significantly higher than that in the healthy group. The difference revealed that although both processes were destructive inflammatory reactions in the periodontium, the pathomechanisms were different and the variation started from the transcription level of the cFn gene.

show abstract

Section: Discussionmentioning

confidence: 99%

Expression of cellular fibronectin mRNA in adult periodontitis and peri-implantitis: a real-time polymerase chain reaction study

Cao

Kang

et al. 2013

Int J Oral Sci

View full text Add to dashboard Cite

show abstract

“…Ujiie et al (2007) have also recently investigated the effect of treatment of PDL specimens with elastase on the mechanical strength [48]. They found that in vitro treatment with elastase increased fragmentation of the oxytalan fibres (Figure 7) and reduced the mechanical strength of the PDL specimen (Figure 8) in a concentration-dependent manner.…”

Section: The Mechanical Strength Of the Pdlmentioning

confidence: 99%

“…d: dentine; b: bone. * P < .05 compared with the control; † P < .05 compared with the elastase-treated specimens, reproduced from Ujiie et al (2008) [48], by permission.…”

Section: Figurementioning

confidence: 99%

See 1 more Smart Citation

Mechanical Strength and Viscoelastic Response of the Periodontal Ligament in Relation to Structure

Komatsu¹

2010

Journal of Dental Biomechanics

View full text Add to dashboard Cite

The mechanical strength of the periodontal ligament (PDL) was first measured as force required to extract a tooth from its socket using human specimens. Thereafter, tooth-PDL-bone preparations have extensively been used for measurement of the mechanical response of the PDL. In vitro treatments of such specimens with specific enzymes allowed one to investigate into the roles of the structural components in the mechanical support of the PDL. The viscoelastic responses of the PDL may be examined by analysis of the stress-relaxation. Video polarised microscopy suggested that the collagen molecules and fibrils in the stretched fibre bundles progressively align along the deformation direction during the relaxation. The stress-relaxation process of the PDL can be well expressed by a function with three exponential decay terms. Analysis after in vitro digestion of the collagen fibres by collagenase revealed that the collagen fibre components may play an important role in the long-term relaxation component of the stress-relaxation process of the PDL. The dynamic measurements of the viscoelastic properties of the PDL have recently suggested that the PDL can absorb more energy in compression than in shear and tension. These viscoelastic mechanisms of the PDL tissue could reduce the risk of injury to the PDL.

show abstract

“…Therefore, tension strain functionally regulates the microfibril assembly in periodontal ligament fibroblasts and thus may contribute to the homeostasis of oxytalan fibers in periodontal ligaments (Tsuruga et al, 2009). Likewise, increased neutrophil elastase observed in periodontal disease degrades the oxytalan fibers and interfibrillar substances in the periodontal ligament to decrease its mechanical strength (Ujiie et al, 2008).…”

Section: Discussionmentioning

confidence: 99%

The Microfibril-Elastin Fiber System Distribution in Left Atrioventricular Valve of the Rat

et al. 2011

View full text Add to dashboard Cite

SUMMARY:The microfibril-elastin fiber system, an important constituent of the extracellular matrix, was studied in the rat left atrioventricular valve to investigate the interrelationship of oxytalan, elaunin and elastic fibers in left atrioventricular valve morphology. The elastin fibers forms continuous bundles observed along the length of the valve in atrial and ventricular layers and oriented parallel to endothelium. The elaunin and oxytalan fibers are distributed in the thickest fiber bundles along the length of the valve. The thinner fibers which radiated towards both the atrial and spongiosa layers, either as isolated or arborescent fiber bundles were identified as oxytalan fibers. With transmission electron microscopy elastic fibers were seen mainly in the atrial layer. The spongiosa layer was composed of elaunin and oxytalan fibers and ventricular layer showed elaunin fibers arranged in continuous bundles parallel to the endothelium. Both fibrillin and elastin were seen and identified by immunocytochemistry with colloidal gold in the left atrioventricular valve spongiosa and atrial layers. These observations allow us to suggest that the microfibril-elastin fiber system plays a role in the mechanical protection and maintenance of the integrity of the rat left atrioventricular valve.

show abstract

Degradation of noncollagenous components by neutrophil elastase reduces the mechanical strength of rat periodontal ligament

Abstract: These results suggest that the increased neutrophil elastase observed in periodontal disease degrades the oxytalan fibers and interfibrillar substances in the periodontal ligament to decrease its mechanical strength.

Cited by 21 publications

References 42 publications

Expression of cellular fibronectin mRNA in adult periodontitis and peri-implantitis: a real-time polymerase chain reaction study

Expression of cellular fibronectin mRNA in adult periodontitis and peri-implantitis: a real-time polymerase chain reaction study

Mechanical Strength and Viscoelastic Response of the Periodontal Ligament in Relation to Structure

The Microfibril-Elastin Fiber System Distribution in Left Atrioventricular Valve of the Rat

Contact Info

Product

Resources

About