Degradation of haloacetic acids with the Fenton-like and analysis by GC-MS: use of bioassays for monitoring of genotoxic, mutagenic and cytotoxic effects

Mourão, Amanda Oliveira; Silva, Diego Francis; Rodríguez, Mariandry; Torres, Thamyris Souza; Franco, Elton Santos; Pádua, Valter Lúcio de; Faria, Márcia Cristina da Silva; Maia, Luiz Fernando Oliveira; Rodrigues, Jairo Lisboa

doi:10.1007/s10661-019-7642-6

Cited by 5 publications

(2 citation statements)

References 34 publications

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“…HAAs come either from brominated and/or chlorinated organic halogen compounds. These organic halogenated compounds are important by-products of the reaction between organic compounds present in water and chlorine in water-treatment plants [27]. There are five contaminating by-products of halogenated organic compounds, which exceed the maximum levels established by the US Environmental Protection Agency (60 µg•L −1 ).…”

Section: Introductionmentioning

confidence: 99%

Isolation and Characterization of a Novel Bacterium from the Marine Environment for Trichloroacetic Acid Bioremediation

et al. 2020

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Halogenated compounds are an important class of environmental pollutants that are widely used in industrial chemicals such as solvents, herbicides, and pesticides. Many studies have been carried out to explore the biodegradation of these chemicals. Trichloroacetic acid (TCA) is one of the main halogenated compounds that are carcinogenic to humans and animals. The bacterium was isolated from the northern coastline of Johor Strait. In this study, the ability of strain MH2 to biodegrade TCA was evaluated by a growth experiment and dehalogenase enzyme assay. The growth profile of the isolated strain was examined. The doubling time for L. boronitolerans MH2 was found to be 32 h. The release of chloride ion in the degradation process was measured at 0.33 × 10−3 ± 0.03 mol∙L−1 after 96 h when the growth curve had reached its maximum within the late bacterial exponential phase. The results showed that the strain had a promising ability to degrade TCA by producing dehalogenase enzyme when cell-free extracts were prepared from growth on TCA as the sole carbon source with enzyme-specific activity, 1.1 ± 0.05 µmolCl−min−1∙mg−1 protein. Furthermore, the morphological, and biochemical aspects of the isolated bacterium were studied to identify and characterize the strain. The morphological observation of the isolated bacterium was seen to be a rod-shaped, Gram-positive, motile, heterotrophic, and spore-forming bacterium. The amplification of the 16S rRNA and gene analysis results indicated that the isolated bacterium had 98% similarity to Lysinibacillus boronitolerans. The morphological and biochemical tests supported the 16S rRNA gene amplification. To the best of the authors’ knowledge, this is the first reported case of this genus of bacteria to degrade this type of halogenated compound.

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Section: Introductionmentioning

confidence: 99%

Isolation and Characterization of a Novel Bacterium from the Marine Environment for Trichloroacetic Acid Bioremediation

et al. 2020

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“…The Allium cepa test is an important bioassay to monitor the potential synergistic effects of a mixture of pollutants, including toxic metals (FISKESJÖ 1985; Leme and Marin-Morales 2009), allowing the determination of the cytotoxicity, genotoxicity, and mutagenicity of As present in water (Hott et al 2020) (HOTT, 2020). The present study was carried out according to methods described in previous studies with modi cation (Grant 1982;Blanc et al 2013;Mourão et al 2019).…”

Section: Allium Cepa Testmentioning

confidence: 99%

Arsenic in Mining Area: Environmental Contamination Route

Hott¹,

Oliveira²,

Bomfeti³

et al. 2021

Preprint

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The emission of residues has contributed immensely to the fact that man is susceptible and exposed to toxic chemical products, among them, arsenic. One of the main anthropic sources of arsenic in the world is mining, which can contribute to the contamination of soil, water, air, and food. An example of environmental arsenic contamination in Brazil occurs in the city of Paracatu - MG, due to the operation of a gold mine. Thus, the objectives of this study were: (i) to assess the physical-chemical parameters and the concentration of arsenic in samples of superficial fresh water (sub-basin of the Paracatu River), and soil samples, during winter and summer; (ii) evaluate the arsenic concentration in samples of particulate matter; (iii) determine the concentration and species of As in biological samples and (iiii) evaluate the toxicity of surface waters using the Allium cepa test. There was no change in the physical-chemical parameters. The water samples collected during the winter and all soil samples showed values of As above those allowed by organs regulatory agencies. The values of As in the particulate material showed great variations between the regions under study but were within the limit established by the ATSDR for urban areas. Inorganic and organic As was found above the limit allowed in biological samples The analysis of water toxicity by the Allium cepa test indicated the presence of cytotoxic and genotoxic compounds, demonstrating a great risk for the population exposed to the waters of the Rico stream.

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Purification of arsenic-contaminated water using iron molybdate filters and monitoring of their genotoxic, mutagenic, and cytotoxic effects through bioassays

Hott

Magalhães

Maia

et al. 2020

Environ Sci Pollut Res

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Degradation of haloacetic acids with the Fenton-like and analysis by GC-MS: use of bioassays for monitoring of genotoxic, mutagenic and cytotoxic effects

Cited by 5 publications

References 34 publications

Isolation and Characterization of a Novel Bacterium from the Marine Environment for Trichloroacetic Acid Bioremediation

Isolation and Characterization of a Novel Bacterium from the Marine Environment for Trichloroacetic Acid Bioremediation

Arsenic in Mining Area: Environmental Contamination Route

Purification of arsenic-contaminated water using iron molybdate filters and monitoring of their genotoxic, mutagenic, and cytotoxic effects through bioassays

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