Degradation of cyclohexylamine by a new isolate of Pseudomonas plecoglossicida

Shen, Yi; Ding, Yan; Chi, Xiang-Qun; Yang, Ya-Chun; Leak, David J.; Zhou, Ning‐Yi

doi:10.1007/s11274-007-9651-9

Cited by 10 publications

(3 citation statements)

References 10 publications

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“…The enzyme has great potential in eliminating CHAM pollution ( Iwaki et al, 1999b ; Yan et al, 2017 ) and synthesis of chiral amines in pharmaceutical industry ( Li et al, 2014 ; Li et al, 2016 ). Thus far, only a few CHAOs have been cloned and characterized ( Iwaki et al, 1999b ; Shen et al, 2008 ; Mirza et al, 2013 ; Li et al, 2016 ; Yan et al, 2017 , 2018 ). The limited enzyme resource cannot satisfy the requirement of their biotechnological applications.…”

Section: Discussionmentioning

confidence: 99%

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Characterization of a New Cyclohexylamine Oxidase From Acinetobacter sp. YT-02

et al. 2018

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Cyclohexylamine (CHAM) is widely used in various industries, but it is harmful to human beings and the environment. Acinetobacter sp. YT-02 can degrade CHAM via cyclohexanone as an intermediate. In this study, the cyclohexylamine oxidase (CHAO) gene from Acinetobacter sp. YT-02 was cloned. Amino acid sequence alignment indicated that the cyclohexylamine oxidase (CHAOYT–02) was 48% identical to its homolog from Brevibacterium oxydans IH-35A (CHAOIH–35). The enzyme was expressed in Escherichia coli BL21 (DE3), and purified to apparent homogeneity by Ni-affinity chromatography. The purified enzyme was proposed to be a dimer of molecular mass of approximately 91 kDa. The enzyme exhibited its maximum activity at 50°C and at pH 7.0. The enzyme was thermolabile as demonstrated by loss of important percentage of its maximal activity after 30 min incubation at 50°C. Metal ions Mg2+, Co2+, and K+ had certain inhibitory effect on the enzyme activity. The kinetic parameters Km and Vmax were 0.25 ± 0.02 mM and 4.3 ± 0.083 μM min−1, respectively. The biochemical properties, substrate specificities, and three-dimensional structures of CHAOYT–02 and CHAOIH–35 were compared. Our results are helpful to elucidate the mechanism of microbial degradation of CHAM in the strain YT-02. In addition, CHAOYT–02, as a potential biocatalyst, is promising in controlling CHAM pollution and deracemization of chiral amines.

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Section: Discussionmentioning

confidence: 99%

“…These bacteria included Brevibacterium oxydans IH-35A ( Iwaki et al, 1999a ). Pseudomonas plecoglossicida NyZ12 ( Shen et al, 2008 ; Yan et al, 2017 ), and Acinetobacter sp. YT-02 isolated by our group ( Yan et al, 2018 ).…”

Section: Introductionmentioning

confidence: 99%

Characterization of a New Cyclohexylamine Oxidase From Acinetobacter sp. YT-02

et al. 2018

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“…Cyclohexylamine (C 6 H 13 N) is considered an important organic intermediate that is employed as an antiseptic in various industries to make plasticizers, insecticides, and dyes (Shen et al, 2008). Its properties such as toxicity have received consideration.…”

Section: Introductionmentioning

confidence: 99%

Study the influence of experimental conditions on the removal of organic compound in the Fenton process

Al-Kaabi

2023

ECB

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The oxidation of cyclohexylamine (CHA) was carried out in a batch reactor, using Fenton's reagent. The effects of the following parameters were investigated: residence time, pH level, ferrous ion concentration, and hydrogen peroxide concentration. Residence time ranged from 30 min to 2 h, and its effect was found to be significantly positive. A pH of 3 for the medium was observed to be optimum. Ferrous ion and hydrogen peroxide concentrations ranged from 0.1 g/l to 0.265 g/l and from 0.5 g/l to 1.3 g/l, respectively. The influence of hydrogen peroxide was significant at 0.16 g/l of ferrous ions and a pH of 3. CHA removal efficiency was measured by calculating chemical oxygen demand (COD). The maximum COD removal efficiency was 62%, and the minimum yield of ammonia was 14%. The yields of nitrite and nitrate were negligible. Overall, the results proved the effectiveness of the Fenton process for the treatment of CHA.

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