Degradation of Bisphenol a Using Horseradish Peroxidase Immobilized on Fe-based MOFs

Abstract

In this study, we synthesized a water-stable Fe-based metal-organic framework, MIL-88B (Fe) by a solvothermal method, and for the first time, MIL-88B (Fe)/HRP composite was prepared for the degradation of bisphenol A (BPA) by immobilizing horseradish peroxidase on MIL-88B (Fe) using covalent fixation method. The material was characterized via XRD, FTIR, TG, and SEM methods. The results showed that the composite could remove bisphenol A quickly and efficiently by adding the hydrophilic agent polyethylene glycol, with the removal rate of BPA up to 99.2% within 1 hour. In addition, the initial concentration of bisphenol A, the dosage of the immobilized enzyme and the amount of H2O2 added had a great influence on the degradation efficiency. It was found that immobilized HRP could be reused, and its storage stability and thermal stability were better than free HRP. These show that immobilized enzymes have a broad application prospect in waste-water treatment.