Degradation of aviation fuel by microorganisms isolated from tropical polluted soils

Omotayo, A. E.; Efetie, OA; Oyetibo, Ganiyu Oladunjoye; Ilori, M.O.; Amund, O.O

doi:10.4314/ijbcs.v5i2.72133

Cited by 12 publications

(12 citation statements)

References 17 publications

(20 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…[28], which proved that the existence of microorganism is also dependent of the level of TOC as one of the major source of nutrient. Table: 2 showed that the bacterial load was high for all samples, this may be because the crude oil contaminated soil favored the growth of the organisms present in the soil sample, similar results were seen in the research conducted by Omotayo et al [27]. The water holding capacity ranged from 22.4 -32.0% the high value obtained was because of the high organic matter content, which is considered integral in the capacity of a soil to maximize water storage through its effect on creating and stabilizing soil pores and its absorption capacity.…”

Section: Discussionsupporting

confidence: 82%

Biodegradation of Crude Oil-Polluted Soil by Bacterial Isolates from Nigeria

Nkiru¹

2019

FEM

View full text Add to dashboard Cite

Biodegradation of crude oil-polluted soils by bacterial isolates was carried out experimentally, to determine the ability of bacterial isolate to utilize and detoxify crude oil-polluted soils. Three soil samples were collected from crude oilpolluted sites of Owazangboko, Abia State, Aguleri-otu, Anambra State and Obi-igbo, Rivers State, Nigeria. Microbiological analysis and physico-chemical analysis were carried out in the Microbiology Laboratory of Chukwuemeka Odumegwu Ojukwu University, Uli. Physico-chemical analysis was carried out to determine the pH, moisture content, water holding capacity and total organic carbon of the soil samples. Microbiological analysis was carried out using serial dilution and spread plate methods to determine the total viable count, enumeration of hydrocarbon utilizing bacteria (HUB) was done using vapour phase method on mineral salt agar monitored at the wavelength of 600nm for 5 days. Adaptation test was carried out and monitored for 5 days using mineral salt broth containing 1ml and 2ml of sterile crude oil separately. A consortium of six effective isolates was taken for the biodegradation of the crude oil, which was monitored for 14 days. Biochemical tests were carried out on the isolates. Polymerase chain reaction (PCR) technique was used to confirm the identity of the isolates. Gas chromatographic flame ionization detector (GC-FID) was used to monitor the amount of the residual total petroleum hydrocarbon after the biodegradation study. Results of physic-chemical analysis showed; pH value of 3.7-6.6, moisture content 1.6 -4.4., water holding capacity 28.0 -32.0., total organic carbon 3.4 -7.1. Results of total viable count were in the range of 7.1×10 6 -5.9×10 10 . Results of vapour phase method ranged from 0.16 -0.66. Adaptability test revealed values, 0.11 -0.54. Biochemical test results revealed Bacillus spp., Pseudomonas spp., Serratia spp., Micrococcus spp., Arthrobacter spp., Proteus spp., Shigella spp. PCR results gave the identity of the organisms to be Lysinibacillus spp. M2c, Serratia marcescens Mb4, Bacillus aerius TPM-23, Proteus mirabilis LS-3 and a new unidentified bacterium. TPH concentrations were in the range of 582.67 mg/l -123.67 mg/l for Lysinibacillus spp. M2c and Serratia marcescens Mb4, 682.65 -203.85 mg/l for Bacillus aerius TPM-23and 753.32 mg/l -324.77 mg/l for Proteus mirabilis LS-3 and the new unidentified bacterium. The effect of bacterial growth across the days showed a p-value of 0.000 less than the α-value 0.01, this concluded that the growth of the bacteria across the days was significant for both vapour phase test and adaptability test. From this study it is evident that Serratia marcescens Mb4 and Lysinibacillus spp. M2c could be used to effectively treat crude oil-polluted environment.

show abstract

Section: Discussionsupporting

confidence: 82%

Biodegradation of Crude Oil-Polluted Soil by Bacterial Isolates from Nigeria

Nkiru¹

2019

FEM

View full text Add to dashboard Cite

show abstract

“…However, Rhodotorula strains were isolated from oil-and petroleum-polluted environments and as contaminants in stored products (Gaylarde et al, 1999;Yemashova et al, 2007) or have been described as being able to degrade oil compounds, with a preference for the saturated fractions represented by the aliphatic hydrocarbons and higher alkanes (Shailubhai et al, 1984;White et al, 1987;van Beilen et al, 2003;Omotayo et al, 2011).…”

Section: Discussionmentioning

confidence: 99%

Phylogenetic analysis and characterization of an alkane-degrading yeast strain isolated from oil-polluted soil

Csutak¹,

Vassu²

2014

Turk J Biol

View full text Add to dashboard Cite

IntroductionThe yeast strains belonging to the Candida, Pichia, Rhodotorula, and Yarrowia genera are successfully used in biotechnology domains related to the food and chemical industries, therapeutics, and bioremediation. The process of bioremediation is based on the ability of microorganisms to assimilate and biodegrade petroleum or crude oil by using the hydrocarbon compounds as main carbon sources in specific metabolic pathways (Vidali, 2001). The final products of this metabolism are sometimes represented by biosurfactants, surface-active molecules produced on the surface of cells whose diverse structure allows them to reduce surface and interfacial tension and to mediate the assimilation of hydrophobic substrates, including hydrocarbons, at the surface of cells (Muthusamy et al., 2008;Kaya et al., 2014). Therefore, the development of knowledge concerning the structure and production of biosurfactants is important for the improvement of bioremediation technologies in oil recovery, agriculture, cosmetics, therapeutics, and microbial lipase production

show abstract

“…Chikere and Ekwuabu [22] carried out the culture-dependent characterization of hydrocarbon utilizing bacteria in the selected crude-oil impacted sites in Bodo, Ogoniland, Nigeria and reported the presence of Micrococcus luteus, Pseudomonas aeruginosa, Proteus vulgaris and Serratia marcescens which utilized hydrocarbons. Omotayo et al [23] in their work on the degradation of aviation fuel by microorganisms isolated from tropical polluted soils and Yakubu [19] in their work on the biodegradation of Lagoma crude oil using pig dung isolated similar organisms and reported that they had petroleum hydrocarbon-degrading potentials.…”

Section: Discussionmentioning

confidence: 99%

“…Chikere and Azubike [25] in their work on the characterization of hydrocarbon-utilizing fungi from hydrocarbon polluted sediments and water isolated Aspergillus, Penicillium, Cladosporium and Candida. Omotayo et al [23] also isolated Candida tropicalis from tropical polluted soils and reported that it degraded aviation fuel while Candida spp. have been reported by Amund and Nwokoye [26] [30] studied the bacterial degradation of crude oil by gravimetric analysis and reported that bacteria contribute largely in the degradation of hydrocarbons and its compounds.…”

Section: Discussionmentioning

confidence: 99%

Biodeterioration Potentials of Microorganisms Isolated from Pig Manure Obtained at Awka, Nigeria

Samuel¹,

Christian²,

Frederick³

2017

nrc

View full text Add to dashboard Cite

The biodeterioration potentials of microorganisms isolated from pig manure was studied using standard methods. The heterotrophic microorganisms isolated from the manure were Escherichia coli, Micrococcus luteus, Enterococcus faecalis, Staphylococcus aureus, Proteus vulgaris, Shigella flexneri, Serratia marcescens, Pseudomonas aeruginosa, Aspergillus niger, Cladosporium resinae, Penicillium expansum, Trichoderma herbarum and Candida tropicalis while all the organisms except Escherichia coli and Staphylococcus aureus were the hydrocarbon-utilizing isolates. The most active degraders were Pseudomonas aeruginosa, Micrococcus luteus, Aspergillus niger, Cladosporium resinae and the bacterial and fungal consortia that produced the highest turbidity and clarity indicating hydrocarbon utilization. The growth profile of Micrococcus luteus, Pseudomonas aeruginosa, Aspergillus niger and Cladosporium resinae as well as the fungal and bacterial consortia during 35 days of growth in mineral salts-oil medium showed that there was a decrease in the pH and an increase in the viable count of the medium. The highest percentage degradation of the crude oil was by the bacterial consortium (62.0%). This work showed that Pig manure which is generally regarded as a waste product harbours diverse bacteria and fungi which will be useful in remediating oil-polluted environments when applied singly and as a consortia.

show abstract

Degradation of aviation fuel by microorganisms isolated from tropical polluted soils

Cited by 12 publications

References 17 publications

Biodegradation of Crude Oil-Polluted Soil by Bacterial Isolates from Nigeria

Biodegradation of Crude Oil-Polluted Soil by Bacterial Isolates from Nigeria

Phylogenetic analysis and characterization of an alkane-degrading yeast strain isolated from oil-polluted soil

Biodeterioration Potentials of Microorganisms Isolated from Pig Manure Obtained at Awka, Nigeria

Contact Info

Product

Resources

About