Degradation of Allicin as Urease Inhibitor Affected by Soil Conditions and Its Effect on Kinetic Properties of Soil Urease

Salehuddin, Nurliana Farhana; Mansor, Nurlidia; Yahya, Wan Zaireen Nisa; Affendi, Nur Mahfuzah Noor; Manogaran, M. Devendran

doi:10.1080/00103624.2019.1695826

Cited by 4 publications

(2 citation statements)

References 28 publications

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“…The compound could only delay the urea hydrolysis in a shorter period than expected, with no difference from the control sample. The latter results are in line with the findings obtained by Salehuddin et al (2020) who showed that the effectiveness of allicin in inhibiting urease declined after 3 days of soil incubation with no significant difference in terms of the catalytic capacity of urease with control. This can be related to the unstable nature of allicin, which is influenced by the soil conditions such as pH, temperature, and moisture.…”

Section: Discussionsupporting

confidence: 92%

“…The soil was air-dried, passed through a 2-mm sieve and stored for analysis. The physicochemical characteristics of the soil were determined by using the methods described by Salehuddin et al (2020) and are shown in Table 1. In order to restore microbial activities, the soil samples were remoistened at 15% (w/w) soil moisture content (SMC) and incubated at 25 ºC for 21 days (Juan et al 2010).…”

Section: Soil Samplingmentioning

confidence: 99%

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Organosulfur Compounds as Soil Urease Inhibitors and Their Effect on Kinetics of Urea Hydrolysis

Salehuddin

Mansor

Yahya

et al. 2021

J Soil Sci Plant Nutr

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The application of urea fertilizer on soil surfaces causes hydrolysis reactions to occur rapidly, thus releasing ammonia gas to the atmosphere and, in turn, reducing the availability of nitrogen for plant uptake. The urease inhibitor application with urea is considered a mitigation method to reduce the loss of nitrogen. Chemical-based urease inhibitors effectively reduce nitrogen loss, but they also demonstrate adverse side effects on plants. Thus, there is a need to discover potential candidates for urease inhibitors from natural sources. This study evaluates the potential of organosulfur compounds from garlic, namely, allicin, diallyl sulfide (DAS), and diallyl disulfide (DADS), as inhibitors to urea hydrolysis. The effect of allicin, DAS, and DADS on kinetic parameters of soil urease and urea hydrolysis rate was evaluated using the Lineweaver-Burk plot and incubation study. All tested organosulfur compounds exhibited mixed-type inhibition with a high Michaelis constant (K m ) and low maximum velocity of reaction (V max ), compared with control (urea). The calculated inhibitory (dissociation) constant (K i ) for allicin, DAS, and DADS were 0.53, 0.92, and 0.40 mM, respectively. This indicates that DADS is the most potent soil urease inhibitor, followed by allicin and DAS. After DADS treatment, about 46% of the urea-nitrogen (N) content remained after 21 days. Meanwhile, allicin and DAS treatments retained urea-N in the soil for 7 and 11 days, respectively. The results demonstrate that DADS is more effective in delaying enzymatic urea hydrolysis compared with allicin and DAS and showed a high potential as a bio-based urease inhibitor in agriculture.

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Section: Discussionsupporting

confidence: 92%

Section: Soil Samplingmentioning

confidence: 99%