Degradasi Plastik Oleh Jamur Aspergillus terreus (LM 1021) Pada pH 5 dan pH 6; Serta Suhu 25 dan 35 Celcius

Rohmah, Ulin Maulidatul; Shovitri, Maya; Kuswytasari, Kuswytasari

doi:10.12962/j23373520.v7i2.37207

Cited by 3 publications

(7 citation statements)

References 3 publications

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“…Aspergillus niger has also been used by other researchers in degrading bioplastics by burial method with compost soil as a source of microbes and can reduce the weight of bioplastics 29.89% with the sample color becoming darker and brittle after 10 days of observation [22]. Furthermore, Aspergillus terreus (LM 1021) was able to degrade PHB plastic by 44.96%, plastic bags by 4.28% and pure LDPE by 4.9% [23]. The ability of the isolates of A. flavus DFSP.J1 and A. niger DFSP.J4 was higher than that of A. terreus LM 1021.…”

Section: Discussionmentioning

confidence: 99%

“…This is due to its ability to grow even though it is not on a specific substrate, even under acidic environmental conditions. Under stressful conditions, fungi are able to produce several enzymes that can be used to degrade organic compounds [23]. Most fungi have lignolytic enzymes, namely laccase, lignin peroxidase and manganese peroxidase which are extracellular enzymes and have been studied for their ability to degrade plastics [26].…”

Section: Discussionmentioning

confidence: 99%

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Optimization of the Degradation Power of Aspergillus flavus DFSP.J1 and Aspergillus niger DFSP. J4 in Degrading Sago Bioplastics

Gunaedi¹,

Mawardi²,

Rumbrawer³

2021

AJOB

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Bioplastics made from sago flour have the potential to be developed into plastic-based industrial materials that are environmentally friendly and easily degraded by microorganisms. A. flavus DSFP.J1 and A. niger DFSP.J4 are microorganisms from a type of fungus that are proven to be able to degrade sago bioplastics, but have not yet obtained optimal degradation power. Therefore, this study aimed to obtain optimal conditions of the two isolates of fungi in degrading bioplastics made from sago flour at variable inoculum concentrations of 10, 20, 30,40, 50% v/w. The manufacture of sago bioplastics is done by adding acetic acid as a catalyst and added glycerol to form plastic properties in 15% sago flour, after being formed and cut to a size of 1 cm2. Observations to determine the degradation power were carried out after an incubation period of seven days to the next five weeks. The results obtained showed that the optimal conditions of A. flavus DFSP.J1 and in degrading sago bioplastics at 40% inoculum concentration with a degradation power of 56.52% in the fifth week of observation and tended to increase the degradation power on the following day, as well as A. niger DFSP.J4 optimally degrades at 30% inoculum concentration with a degradation power of 55.90% in the fifth week of observation. The results of the research, it can be concluded. Both the fungus A. flavus DFSP. J1 and A. niger DFSP.J4 showed that potentialy to be developed for further research to increase its degradation power higher and faster.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Optimization of the Degradation Power of Aspergillus flavus DFSP.J1 and Aspergillus niger DFSP. J4 in Degrading Sago Bioplastics

Gunaedi¹,

Mawardi²,

Rumbrawer³

2021

AJOB

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“…Selain itu, Asperigillus niger memiliki potensi degradasi sekitar 6,3% selama masa inkubasi 45 hari. Rohmah et al (2019) menyebutkan bahwa Asperigillus terreus menghasilkan persentase degradasi plastik tertinggi sekitar 3,25% dalam 30 hari masa inkubasi. Berdasarkan hal-hal tersebut, dapat dikatakan bahwa mikroba dari tanah tercemar berpotensi sebagai agen biologis untuk mengurai plastik.…”

Section: Pendahuluanunclassified

“…Potongan plastik dimasukkan ke dalam botol akuabides yang berisi 135 mL NB dan ditambahkan lima ose isolat bakteri. Lalu potongan plastik tersebut diinkubasi selama 4 bulan dalam keadaan statis (Rohmah et al, 2019).…”

Section: Uji Degradasi Polimer Hdpeunclassified

Skrining Mikroba Pendegradasi Plastik Dari Tanah Dan Uji Biodegradasi Dengan Fourier Transform Infrared (Ftir)

Asmi

Baharuddin

Febryanti

2022

Al-Kauniyah J. Biol.

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AbstrakSaat ini pencemaran lingkungan telah menjadi permasalahan global, tidak hanya dirasakan oleh negara maju tetapi juga dialami oleh negara berkembang. Penyebab terjadinya pencemaran tersebut ialah adanya benda asing masuk ke dalam lingkungan, sehingga mengakibatkan kerusakan komposisi dari komposisi awalnya. Salah satu penyebab pencemaran lingkungan adalah kehadiran limbah plastik. Limbah tersebut merupakan limbah yang sangat sulit didegradasi. Salah satu solusi alternatif yang dilakukan dalam mendegradasi plastik adalah dengan menggunakan mikroorganisme. Penelitian ini bertujuan untuk melakukan skrining mikroorganisme dalam mendegradasi plastik. Jenis plastik yang digunakan sebagai sampel adalah High-Density Polyethylene (HDPE). Metode yang digunakan dalam penelitian ini adalah skrining mikroba dan uji biodegradasi dengan memanfaatkan Fourier Transform Infrared Spectroscopy (FTIR). Hasil yang diperoleh dalam studi ini adalah terdapat 17 jumlah isolat. Zona bening besar ditunjukkan oleh isolat A12P. Potensi biodegradasi terbesar selama 4 bulan dengan persentase 17,9104% ditunjukkan oleh isolat A12P. Hasil degradasi menunjukkan bahwa kehadiran gugus C=O mengindikasikan terjadinya reaksi oksidasi dan kinerja enzim oleh mikroba. Oleh karena itu, isolat A12P memiliki potensi untuk mendegradasi plastik.AbstractNowadays environmental pollution has become a global problem, which is not only experienced by developed countries but also by developing countries. The cause of this pollution is the presence of foreign objects entering the environment resulting in damage to the composition of the original composition. One of the causes of environmental pollution is the presence of plastic waste. This waste is very difficult to degrade. One of the alternative solutions to degrade plastics is to use microorganisms. This study aims to screen microorganisms to degrade plastics. The type of plastic used as the sample was High-Density Polyethylene (HDPE). The method used in this research was microbial growth screening and biodegradation testing using Fourier transform infrared spectroscopy (FTIR). The results obtained 17 isolates. The largest clear zone was shown by isolate A12P. The greatest biodegradation potential for 4 months with a percentage of 17.9104% was shown by isolate A12P. The results of degradation indicated that the presence of the C=O group caused the occurrence of oxidation reactions and enzyme performance by microbes. Therefore, A12P isolate has the potential to degrade plastics.

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“…HDPE banyak dimanfaatkan dalam aplikasi industri seperti kantong plastik, botol susu, botol detergen, pipa air, dll (Balasubramanian et al, 2010). Karakteristik HDPE yang cenderung hidrofobik inilah yang menyebabkan tingkat degradabilitas HDPE rendah sehingga tidak dapat terurai secara alami bahkan hingga ribuan tahun (Rohmah et al, 2019). Beberapa penelitian menunjukkan plastik HDPE yang berada dilautan selama hampir 100 tahun hanya mengalami degradasi parsial yang artinya tidak ada perubahan besar pada struktur permukaan HDPE (Otake et al, 1995;Cichy et al, 2015).…”

Section: Latar Belakangunclassified

Pengaruh mikroplastik polietilen dan oxo-degradable (Oxium) pada pertumbuhan Mikroalga Tetraselmis Chuii

et al. 2021

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Salah satu cara yang digunakan di Indonesia dalam menanggulangi berlimpahnya jumlah sampah plastik di lingkungan perairan adalah dengan menggantikan kantong plastik berbahan polimer polietilen (PE) dengan plastik oxodegradable yang disebut oxium. Penelitian ini dilakukan dengan tujuan untuk melihat pengaruh mikroplastik polietilen jenis HDPE (High Density Polyethylene) dengan plastic oxodegradable oxium. Penelitian dilakukan dengan menggunakan mikroalga Tetraselmis chuii sebagai mikroorganisme yang akan mendapat perlakuan mikroplastik dengan konsentrasi yang berbeda. Dari Hasil pengukuran optical density untuk menentukan laju pertumbuhan mikroalga Tetraselmis Chuii menunjukkan bahwa laju pertumbuhan Tetraselmis dengan perlakuan mikroplastik polietilen mengalami penurunan yang signifikan dibandingkan dengan mikroplastik oxium. Konsentrasi mikroplastik ikut berperan dalam menentukan laju pertumbuhan Tetraselmis chuii di mana pada perlakuan mikroplastik oxium terjadi penurunan hingga 37,66% pada konsentrasi mikroplastik 300mg/500mL dan 81,70% pada perlakuan mikroplastik polietilen dengan konsentrasi 200mg/500mL. Mikroplastik polietilen dan oxium memberikan dampak negatif pada organisme tingkat rendah disebabkan oleh kemampuannya dalam melepas bahan aditif yang bersifat toksik sehingga diperlukan solusi yang lebih baik untuk menggantikan fungsi plastik dengan bahan yang lebih ramah bagi lingkungan hidup. ABSTRACTOne of the methods used in Indonesia in tackling the abundance of plastic waste in the aquatic environment is to replace plastic bags made of polyethylene (PE) polymer with oxodegradable plastic called oxium. This research was conducted with the aim of examining the effect of HDPE (High Density Polyethylene) microplastic polyethylene with oxodegradable oxium plastic. The research was conducted using the microalgae Tetraselmis chuii as microorganisms that will receive microplastic treatment with different concentrations. From the results of optical density measurements to determine the growth rate of Tetraselmis chuii microalgae, it was shown that the growth rate of Tetraselmis with polyethylene microplastics treatment decreased significantly compared to oxium microplastics. The concentration of microplastics played a role in determining the growth rate of Tetraselmis chuii where in the oxium microplastic treatment there was a decrease of up to 37.66% at the microplastic concentration of 300mg/500mL and 81.70% at the polyethylene microplastic treatment with a concentration of 200mg/500mL. Polyethylene and oxyum microplastics have a negative impact on low-level organisms due to their ability to release toxic additives so that better solutions are needed to replace the function of plastics with materials that are more environmentally friendly.

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Degradasi Plastik Oleh Jamur Aspergillus terreus (LM 1021) Pada pH 5 dan pH 6; Serta Suhu 25 dan 35 Celcius

Cited by 3 publications

References 3 publications

Optimization of the Degradation Power of Aspergillus flavus DFSP.J1 and Aspergillus niger DFSP. J4 in Degrading Sago Bioplastics

Optimization of the Degradation Power of Aspergillus flavus DFSP.J1 and Aspergillus niger DFSP. J4 in Degrading Sago Bioplastics

Skrining Mikroba Pendegradasi Plastik Dari Tanah Dan Uji Biodegradasi Dengan Fourier Transform Infrared (Ftir)

Pengaruh mikroplastik polietilen dan oxo-degradable (Oxium) pada pertumbuhan Mikroalga Tetraselmis Chuii

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