Deformation and dynamics of erythrocytes govern their traversal through microfluidic devices with a deterministic lateral displacement architecture

Chien, Wei; Zhang, Zunmin; Gompper, Gerhard; Fedosov, Dmitry

doi:10.1063/1.5112033

Cited by 17 publications

(15 citation statements)

References 54 publications

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“…Chien et al 77 reported modeling studies of a DLD device that employed mesoscopic hydrodynamics of RBCs in DLD devices with circular posts to better understand the interplay between cell behavior in complex microfluidic flow environments and sorting capabilities of such devices. The authors identified regimes of RBC dynamics (e.g., tumbling, tank-treading, and trilobe motion) and presented mode diagrams of RBC behavior (e.g., displacement, zig-zagging, and intermediate modes) while residing in the DLD device.…”

Section: Deterministic Lateral Displacement and Density Gradient Centrifugation Cell Sortingmentioning

confidence: 99%

Cell Separations and Sorting

2019

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The development of new methods and technologies for cell separation, sorting, selection, isolation, or enrichment (many times the aforementioned terms are used interchangeably) are becoming more imperative due in part to the wealth of research aimed at addressing issues for the analysis of liquid biopsy targets, such as rare cells, to realize the concept of "precision medicine". Precision medicine is defined as "treatments targeted to the needs of individual patients on the basis of genetic, biomarker, phenotypic, or psychosocial characteristics that distinguish a patient from others with similar clinical presentations." 1 Before full implementation of precision medicine in a hospital or clinical setting, technological discoveries must be realized to assist in the analysis of disease-associated cells enriched from complex samples.Liquid biopsy markers and the cargo that they carry have been shown to be an attractive source of information that can be used to facilitate precise decisions for disease management. These biomarkers include, but are not limited to, rare cells such as circulating tumor cells (CTCs) or cancer stem cells (CSCs), immune cells (i.e., CD8+ T-cells), bacterial and viral cells, cell-free molecules such as cell free DNA (cfDNA), and extracellular vesicles (EVs). Liquid biopsies are attractive because of the minimally invasive nature of the sampling method (i.e., collection of peripheral blood, urine, saliva) to secure relevant biomarkers. These biomarkers can enable precision medicine decisions on managing a variety of diseases, including oncology and nononcology-related diseases. 2,3 Liquid biopsies *

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Section: Deterministic Lateral Displacement and Density Gradient Centrifugation Cell Sortingmentioning

confidence: 99%

Cell Separations and Sorting

2019

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“…Various numerical methods have been used so far to characterize the flow and particle behavior in DLD microsystems. A distinction can be made between calculations of the steady-state flow field [14,[17][18][19][20][21] and simulations that include particle dynamics [8,15,[22][23][24][25][26][27][28][29][30][31][32][33][34]. The calculation of the velocity field only allows for an estimation of the behavior of particles in the flow and the d c , but the interactions between particles and the fluid are neglected.…”

Section: Introductionmentioning

confidence: 99%

“…The calculation of the velocity field only allows for an estimation of the behavior of particles in the flow and the d c , but the interactions between particles and the fluid are neglected. The fluid-structure interactions are either calculated in direct numerical simulations (DNS) [25][26][27][28][29]32] or the fluid and solid phases are calculated in separate processes, and both methods are coupled iteratively; for example, the lattice Boltzmann method (LBM) coupled with the discrete element method (LBM-DEM) [30] or coupled with the finite element method (FEM) [31]. The fluid-structure simulations were either simplified into two dimensions [8,15,[22][23][24]28,32,34,35] or calculated in a more complex three-dimensional way [25][26][27][29][30][31].…”

Section: Introductionmentioning

confidence: 99%

“…The fluid-structure interactions are either calculated in direct numerical simulations (DNS) [25][26][27][28][29]32] or the fluid and solid phases are calculated in separate processes, and both methods are coupled iteratively; for example, the lattice Boltzmann method (LBM) coupled with the discrete element method (LBM-DEM) [30] or coupled with the finite element method (FEM) [31]. The fluid-structure simulations were either simplified into two dimensions [8,15,[22][23][24]28,32,34,35] or calculated in a more complex three-dimensional way [25][26][27][29][30][31]. In addition, the calculations differ in whether only the forces (and moments) that the fluid exerts on the particles are calculated (one-way coupling) [11,23] or whether particle feedback to the fluid takes place (two-way coupling) [22,24,[26][27][28]36].…”

Section: Introductionmentioning

confidence: 99%

“…The fluid-structure simulations were either simplified into two dimensions [8,15,[22][23][24]28,32,34,35] or calculated in a more complex three-dimensional way [25][26][27][29][30][31]. In addition, the calculations differ in whether only the forces (and moments) that the fluid exerts on the particles are calculated (one-way coupling) [11,23] or whether particle feedback to the fluid takes place (two-way coupling) [22,24,[26][27][28]36].…”

Section: Introductionmentioning

confidence: 99%

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Simulative Investigation of Different DLD Microsystem Designs with Increased Reynolds Numbers Using a Two-Way Coupled IBM-CFD/6-DOF Approach

et al. 2022

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Deterministic lateral displacement (DLD) microsystems are suitable for the size fractionation of particle suspensions in the size range of 0.1 to 10 µm. To be able to fractionate real particles beyond a laboratory scale, these systems have to be designed for higher throughputs. High flow resistances and increasing the clogging of the systems impose substantial challenges for industrial operation. Simulative parameter studies are suitable for improving the design of the systems; for example, the position and shape of the posts. A high-resolution, two-way coupled 6-DOF CFD-DEM approach was used to study the flow and particle behavior of different post shapes (circular and triangular) and post sizes at different Reynolds numbers. The results were compared with the classical first streamline width theory. It was shown that the streamline theory does not account for all effects responsible for the separation. Furthermore, a shift in the critical particle diameter to smaller values could be obtained when increasing the Reynolds number and also when using triangular posts with reduced post sizes compared to the post spacing. These findings can help to improve the efficiency of the systems as the post spacing could be extended, thus reducing the flow resistance and the probability of clogging.

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Performance optimization of a DLD microfluidic device for separating deformable CTCs

Mohammadali,

Bayareh,

Nadooshan

2024

Electrophoresis

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Deterministic lateral displacement (DLD) microfluidic devices work based on the streamlines created by an array of micro‐posts. The configuration of pillars alters the isolation efficiency of these devices. The present paper optimizes the performance of a DLD device for isolating deformable circulating tumor cells. The input variables include cell diameter (d), Young's modulus (), Reynolds number (Re), and tan θ, where θ is the tilted angle of micro‐posts. The output, which is the response of the system, is DLD. The numerical simulation results are employed to optimize the device using the response surface method, leading to the proposition of a correlation to estimate DLD as a function of input variables. It is demonstrated that the maximum and minimum impacts on cell lateral displacement correspond to and Re, respectively.

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Deformation and dynamics of erythrocytes govern their traversal through microfluidic devices with a deterministic lateral displacement architecture

Cited by 17 publications

References 54 publications

Cell Separations and Sorting

Cell Separations and Sorting

Simulative Investigation of Different DLD Microsystem Designs with Increased Reynolds Numbers Using a Two-Way Coupled IBM-CFD/6-DOF Approach

Performance optimization of a DLD microfluidic device for separating deformable CTCs

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