Defining the Genetic Basis of Plant–Endophytic Bacteria Interactions

Piński, Artur; Betekhtin, Alexander; Hupert-Kocurek, Katarzyna; Mur, Luis A. J.; Hasterok, Robert

doi:10.3390/ijms20081947

Cited by 116 publications

(75 citation statements)

References 123 publications

(166 reference statements)

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“…The data on in vitro bacterial-fungal dual-inoculations as well as plant biomass and leaf area from the growth enhancement experiments were analyzed in R 3.5.1 (Team RC 2019). Data distributions were evaluated with the fitdistrplus package (Delignette-Muller and Dutang 2015) and linear or linear mixed-effects models (nlme R package) (Pinheiro et al 2019), when necessary, were created. After graphical validation of homogeneity assumption, ANOVA test was carried out on the generated models, followed by pairwise comparisons (Tukey's method, P = 0.05) calculated by Estimated Marginal Means (emmeans R package) (Lenth 2019).…”

Section: Discussionmentioning

confidence: 99%

Interaction between endophytic Proteobacteria strains and Serendipita indica enhances biocontrol activity against fungal pathogens

et al. 2020

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Aims Plants host communities of fungal and bacterial endophytes, establishing a complex network of multipartite interactions, but the mechanisms whereby they interact are poorly understood. Some fungi, such as the beneficial mycorrhiza-like fungus Serendipita (=Piriformospora) indica, can be helped by bacteria for establishment, survival and colonization. Although this fungus harbors a Rhizobium as an endofungal bacterium, we hypothesized that other bacteria might also establish associations with the fungus and combining S. indica with bacteria might enhance plant growth and health. Methods The interactions among S. indica and four e n d o p h y t i c P r o t e o b a c t e r i a b e l o n g i n g t o Methylobacterium, Tardiphaga, Rhodanobacter and Trinickia spp. were characterized in vitro and for their effect on tomato growth and biocontrol of Fusarium oxysporum and Rhizoctonia solani. Possible mechanisms behind these interactions were described based on genome and microscopic analyses, using fungal and bacterial strains tagged with fluorescent markers. Results All bacteria stimulated S. indica growth in vitro. Moreover, several of the bacteria stimulated growth of tomato plants, but co-inoculations with S. indica and bacteria did not perform better than single inoculations. Contrarily, combinations of S. indica and bacteria significantly reduced disease progression of fungal pathogens. These microbes seem to cooperate in the process of root colonization for instance by increasing fungal sporulation and hyphae expansion, showing multipartite interaction between microbes and plants. Interestingly, the strain of Trinickia internally colonizes spores of S. indica as an endofungal bacterium during in vitro-co-culturing, suggesting further that the fungus might acquire formerly unrecognized genera of bacteria and genome analysis of the bacteria revealed many genes potentially involved in fungal and plant growth stimulation, biocontrol and root colonization, highlighting putative mechanisms of plantfungal-bacterial interaction. Conclusions Our study represents an important step towards unraveling the complex interactions among plants, S. indica, endophytic bacteria and fungal pathogens, and indicates that adding bacteria to fungal inoculum could have a remarkable impact on the plantS. indica symbiosis.

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Section: Discussionmentioning

confidence: 99%

Interaction between endophytic Proteobacteria strains and Serendipita indica enhances biocontrol activity against fungal pathogens

et al. 2020

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“…Bacterial traits enabling life in the endosphere Several bacterial traits enable life in the plant endosphere (Compant et al, 2010;Pinski et al, 2019). Lipopolysaccharides, flagella, pili, and twitching motility (Duijff et al, 1997, Dörr et al, 1998, Böhm et al, 2007Tadra-Sfeir et al, 2011) have been linked to endophytic colonization and are also important for rhizosphere competence (Compant et al, 2010).…”

Section: Colonization Pattern Of Endophytesmentioning

confidence: 99%

The plant endosphere world – bacterial life within plants

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Vacher

et al. 2020

Environmental Microbiology

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The plant endosphere is colonized by complex microbial communities and microorganisms, which colonize the plant interior at least part of their lifetime and are termed endophytes. Their functions range from mutualism to pathogenicity. All plant organs and tissues are generally colonized by bacterial endophytes and their diversity and composition depend on the plant, the plant organ and its physiological conditions, the plant growth stage as well as on the environment. Plant-associated microorganisms, and in particular endophytes, have lately received high attention, because of the increasing awareness of the importance of host-associated microbiota for the functioning and performance of their host. Some endophyte functions are known from mostly lab assays, genome prediction and few metagenome analyses; however, we have limited understanding on in planta activities, particularly considering the diversity of micro-environments and the dynamics of conditions. In our review, we present recent findings on endosphere environments, their physiological conditions and endophyte colonization. Furthermore, we discuss microbial functions, the interaction between endophytes and plants as well as methodological limitations of endophyte research. We also provide an outlook on needs of future research to improve our understanding on the role of microbiota colonizing the endosphere on plant traits and ecosystem functioning.

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“…For years, plant-associated bacteria have been speculated to be the organisms capable of promoting growth and/or suppressing diseases when present in the rhizosphere and as endophytes within healthy plant tissues [1,2]. Leguminous plants have the capability to associate with rhizobia by the nodulation process and fix atmospheric nitrogen (N) in the root nodule.…”

Section: Introductionmentioning

confidence: 99%

Deciphering the Factors for Nodulation and Symbiosis of Mesorhizobium Associated with Cicer arietinum in Northwest India

et al. 2019

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The compatibility between rhizobia and legumes for nitrogen-fixing nodules and the stages of root hair curling, formation of infection thread, and nodulation initiation have been vitally studied, but the factors for the sustainable root surface colonization and efficient symbiosis within chickpea and rhizobia have been poorly investigated. Hence, we aimed to analyze phenotypic properties and phylogenetic relationships of root-nodule bacteria associated with chickpea (Cicer arietinum) in the north-west Indo Gangetic Plains (NW-IGP) region of Uttar Pradesh, India. In this study, 54 isolates were recovered from five agricultural locations. Strains exhibited high exopolysaccharide production and were capable of survival at 15–42 °C. Assays for phosphate solubilization, catalase, oxidase, Indole acetic acid (IAA) production, and 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase activity revealed that all the tested isolates possessed plant growth-promoting potential. Metabolic profiling using Biolog plates indicated that patterns of substrate utilization differed considerably among isolates. A biofilm formation assay showed that isolates displayed a nearly four-fold range in their capacity for biofilm development. Inoculation experiments indicated that all isolates formed nodules on chickpea, but they exhibited more than a two-fold range in symbiotic efficiency. No nodules were observed on four other legumes (Phaseolus vulgaris, Pisum sativum, Lens culinaris, and Vigna mungo). Concatenated sequences from six loci (gap, edD, glnD, gnD, rpoB, and nodC) supported the assignment of all isolates to the species Mesorhizobium ciceri, with strain M. ciceri Ca181 as their closest relative.

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Defining the Genetic Basis of Plant–Endophytic Bacteria Interactions

Cited by 116 publications

References 123 publications

Interaction between endophytic Proteobacteria strains and Serendipita indica enhances biocontrol activity against fungal pathogens

Interaction between endophytic Proteobacteria strains and Serendipita indica enhances biocontrol activity against fungal pathogens

The plant endosphere world – bacterial life within plants

Deciphering the Factors for Nodulation and Symbiosis of Mesorhizobium Associated with Cicer arietinum in Northwest India

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