Defining the Future: Emerging Issues in Biotechnology, Intellectual Property Rights and Technology Transfer

Ganguli, Poulomi; Prickril, Ben; Khanna, Rita

doi:10.1002/9783527627301.ch1

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Electron Transfer Pathway Engineering1

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2024

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“…The most widely used enzymes for cofactor regeneration in commercial processes are formate dehydrogenase (FDH) and glucose dehydrogenase (GDH), whereas phosphite (PDH), alcohol (ADH), and glucose 6-phosphate dehydrogenases (G6PDH) have been tested at laboratory scale [ 40 , 41 ]. Ma et al [ 42 ] mutated formate dehydrogenase from Candida dubliniensis , and the catalytic efficiency of the mutant Cd FDH-M4 was improved 75-fold, with total cofactor turnover numbers (TTN) ranging from 135 to 986.…”

Section: Electron Transfer Pathway Engineeringmentioning

confidence: 99%

Engineering Electron Transfer Pathway of Cytochrome P450s

He,

Liu,

2024

Molecules

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Cytochrome P450s (P450s), a superfamily of heme-containing enzymes, existed in animals, plants, and microorganisms. P450s can catalyze various regional and stereoselective oxidation reactions, which are widely used in natural product biosynthesis, drug metabolism, and biotechnology. In a typical catalytic cycle, P450s use redox proteins or domains to mediate electron transfer from NAD(P)H to heme iron. Therefore, the main factors determining the catalytic efficiency of P450s include not only the P450s themselves but also their redox-partners and electron transfer pathways. In this review, the electron transfer pathway engineering strategies of the P450s catalytic system are reviewed from four aspects: cofactor regeneration, selection of redox-partners, P450s and redox-partner engineering, and electrochemically or photochemically driven electron transfer.

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Section: Electron Transfer Pathway Engineeringmentioning

confidence: 99%