Defense mechanism of heme oxygenase‐1 against cytotoxic and receptor activator of nuclear factor‐κB ligand inducing effects of hydrogen peroxide in human periodontal ligament cells

Abstract

These data suggest that heme oxygenase-1 induction plays a protective role in periodontal ligament cells against the cytotoxic and RANKL-inducing effects of H2O2, through multiple signaling pathways.

The role of heme oxygenase-1 in mechanical stress- and lipopolysaccharide-induced osteogenic differentiation in human periodontal ligament cells

“…[19][20][21][22][23][24] In the present study, we found that MS increased HO-1 mRNA and protein expression in PDLCs. These findings are consistent with our previous study in which osteogenic differentiation Figure 4.…”

“…21,22,24 Increases in the expression of HO-1, BMP-2, BMP-7, and Runx-2 resulting from treatment with LPS and MS were attenuated by preincubation with selective inhibitors of p38, ERK, PI3K, PKG, and NF-kB (SB203580, U0126, LY294002, KT5823, and PDTC, respectively). These results suggest that LPS-induced and MS-induced HO-1 and BMP expression occurs via the activation of p38, ERK MAPK, PI3K, PKG, and NF-kB.…”

“…Previously, we reported that the HO-1 pathway is a key mechanism for the adaptation to stressful conditions and the recovery from injurious events by dental cells. [19][20][21][22][23][24] Moreover, the expression of HO-1 is related to adipogenesis by human MSCs, 25 osteoblastic differentiation by PDLCs 23 and neuronal differentiation by MSCs. 26 Because HO-1 is associated with differentiation, it is plausible to postulate that HO-1 is involved in the response of PDLCs to MS and bacterial infection as well as in the differentiation of PDLCs to osteoblast-like cells.…”

Substance P regulates macrophage inflammatory protein 3α/chemokine C-C ligand 20 (CCL20) with heme oxygenase-1 in human periodontal ligament cells

“…We have reported previously that proinflammatory cytokines [30] and nitric oxide [31] induced expression of HO-1, which potentially plays a role in cytoprotection within human pulp cells. Moreover, induction of HO-1 plays a protective role in PDL cells by combating H2O2 through multiple signalling pathways [32]. HO-1 countering the inductive effects of CCL20 in PDL cells has not been reported.…”

Nitric Oxide Modulates Osteoblastic Differentiation with Heme Oxygenase-1 via the Mitogen Activated Protein Kinase and Nuclear Factor-kappaB Pathways in Human Periodontal Ligament Cells

“…14) One of three mammalian HO isoforms, HO-1 (also called heat shock protein 32), is a stress-responsive protein induced by various agents and is involved in a variety of regulatory and protective mechanisms in cells. 15,16) We previously reported that HO-1 is induced by pro-inflammatory cytokines, NO, substance P, hydrogen peroxide, and mechanical stress, [17][18][19][20][21][22][23] and that it may play a cytoprotective role in human pulp and PDL cells. However, HO-1-mediated NOinductive effects of osteoblastic differentiation in PDL cells has not yet been reported.…”