volume 191, issue 5, P2393-2402 2013
DOI: 10.4049/jimmunol.1301279
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Nancy M. Choi, Salvatore Loguercio, Jiyoti Verma-Gaur, Stephanie C. Degner, Ali Torkamani, Andrew I. Su, Eugene M. Oltz, Maxim Artyomov, Ann J. Feeney

Abstract: A diverse antibody repertoire is formed through the rearrangement of V, D, and J segments at the immunoglobulin heavy chain (Igh) loci. The C57BL/6 murine Igh locus has over 100 functional VH gene segments that can recombine to a rearranged DJH. While the non-random usage of VH genes is well documented, it is not clear what elements determine recombination frequency. To answer this question we conducted deep sequencing of 5′-RACE products of the Igh repertoire in pro-B cells, amplified in an unbiased manner. …

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