Deep Sea Actinomycetes and Their Secondary Metabolites

Kamjam, Manita; Sivalingam, Periyasamy; Deng, Zinxin; Hong, Kui

doi:10.3389/fmicb.2017.00760

Cited by 100 publications

(86 citation statements)

References 86 publications

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“…200-500 m depth and which were once regarded as being biologically inert (Forbes' azoic theory [14]), a notion that was dispelled in the late nineteenth century. Abundant life and biodiversity is now welldocumented even at abyssal (>2 000 m) and hadal (>6 000 m) depths and actinobacteria have been isolated from sediments taken at all of these extremes [8,[15][16][17][18]. Conditions found in these oceans include high pressure, temperature and salinity extremes, low nutrient status and anoxia.…”

Section: Bioprospecting Biomes and Sitesmentioning

confidence: 99%

Dark, rare and inspirational microbial matter in the extremobiosphere: 16 000 m of bioprospecting campaigns

Bull

Goodfellow

2019

Microbiology

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The rationale of our bioprospecting campaigns is that the extremobiosphere, particularly the deep sea and hyper-arid deserts, harbours undiscovered biodiversity that is likely to express novel chemistry and biocatalysts thereby providing opportunities for therapeutic drug and industrial process development. We have focused on actinobacteria because of their frequent role as keystone species in soil ecosystems and their unrivalled track record as a source of bioactive compounds. Population numbers and diversity of actinobacteria in the extremobiosphere are traditionally considered to be low, although they often comprise the dominant bacterial biota. Recent metagenomic evaluation of 'the uncultured microbial majority' has now revealed enormous taxonomic diversity among 'dark' and 'rare' actinobacteria in samples as diverse as sediments from the depths of the Mariana Trench and soils from the heights of the Central Andes. The application of innovative culture and screening options that emphasize rigorous dereplication at each stage of the analysis, and strain prioritization to identify 'gifted' organisms, have been deployed to detect and characterize bioactive hit compounds and sought-after catalysts from this hitherto untapped resource. The rewards include first-in-a-class chemical entities with novel modes of action, as well as a growing microbial seed bank that represents a potentially enormous source of biotechnological and therapeutic innovation.

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Section: Bioprospecting Biomes and Sitesmentioning

confidence: 99%

Dark, rare and inspirational microbial matter in the extremobiosphere: 16 000 m of bioprospecting campaigns

Bull

Goodfellow

2019

Microbiology

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“…Marine bacteria belonging to Actinobacteria are well documented in the aspect of antibiotics or secondary metabolite producers (e.g., Blunt et al, 2013;Abdelmohsen et al, 2014;Kamjam et al, 2017). In this study, Actinobacteria was found to be the predominant phyla with 18.0% in healthy corals whereas its abundance severely affected in the SGA corals with just 6.4%.…”

Section: Resultsmentioning

confidence: 59%

Bacterial Communities Associated With Healthy and Diseased (Skeletal Growth Anomaly) Reef Coral Acropora cytherea From Palk Bay, India

et al. 2020

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Skeletal growth anomalies (SGA) are easily recognizable disease in reef-forming corals which drain energy from the host and end up with mortality. We collected mucus samples of healthy and SGA affected colonies of Acropora cytherea from Palk Bay, the southeast coast of India and investigated the taxonomic composition of mucus-associated bacterial communities using full-length 16S rRNA gene nanopore sequencing. Metagenomic analysis revealed the dominance of Proteobacteria, Firmicutes, and Actinobacteria in both healthy and diseased samples. The proportion of Proteobacteria in diseased samples was almost doubled compared to the healthy one, whereas Acidobacteria (fourth dominant phyla in healthy sample) and Actinobacteria were significantly low in diseased samples. Planctomycetes, Cyanobacteria, and Chloroflexi were some of the other major phyla found in healthy samples which were low in diseased corals. Kruskal-Wallis test indicated high number of putative pathogenic bacteria group viz. Staphylococcus, Salmonella, Klebsiella, and Escherichia in diseased samples compared to healthy coral. The corals perhaps have acquired potential pathogens belong to the phylum Proteobacteria during SGA formation.

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“…Marine natural products show higher incidences of significant bioactivity and chemical novelty when compared with compounds from terrestrial microorganisms. Therefore, marine organisms that can survive in extremes of pressure, salinity and temperature may be important untapped resources of structurally diverse and biologically active secondary metabolites [1,2]. In order to find new sources of novel bioactive compounds, we isolated actinomycete strains from deep-sea sediment samples.…”

mentioning

confidence: 99%

Micromonospora pelagivivens sp. nov., a new species of the genus Micromonospora isolated from deep-sea sediment in Japan

Intra

Panbangred

Inahashi

et al. 2020

International Journal of Systematic and Evolutionary Microbiology

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A novel marine actinomycete, designated strain KJ-029T, was isolated from a marine sediment sample (water depth of 226 m) in Kagoshima, Japan. 16S rRNA gene sequence analysis revealed that the new isolate was most closely related to Micromonospora craniellae LHW 63014T (99.3 % similarity). Phylogenetic analyses of the genus Micromonospora based on 16S rRNA gene sequences showed that strain KJ-029T was clustered with Micromonospora craniellae LHW 63014T and Micromonospora endophytica 202201T. However, digital DNA–DNA hybridization analyses presented low levels of relatedness in the range of 24.8–32.9 % between strain KJ-029T and the above closely related strains. The novel strain contained meso-diaminopimelic acid and 3-OH-diaminopimelic acid, d-glutamic acid, glycine and d-alanine in the cell-wall peptidoglycan. The acyl type of the peptidoglycan was glycolyl and mycolic acids were absent. The major menaquinone was MK-9(H4). The whole-cell sugars consisted of glucose, mannose, xylose and ribose. Phosphatidylethanolamine was detected as the major phospholipid and corresponded to phospholipid type II. The predominant cellular fatty acid was iso-C16 : 0. The DNA G+C content of the genomic DNA was 71.5 mol%. Based on the present polyphasic study, strain KJ-029T represents a novel species of the genus Micromonospora , for which the name Micromonospora pelagivivens sp. nov. is proposed. The type strain is KJ-029T (=NBRC 113519T=TBRC 9233T).

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Deep Sea Actinomycetes and Their Secondary Metabolites

Cited by 100 publications

References 86 publications

Dark, rare and inspirational microbial matter in the extremobiosphere: 16 000 m of bioprospecting campaigns

Dark, rare and inspirational microbial matter in the extremobiosphere: 16 000 m of bioprospecting campaigns

Bacterial Communities Associated With Healthy and Diseased (Skeletal Growth Anomaly) Reef Coral Acropora cytherea From Palk Bay, India

Micromonospora pelagivivens sp. nov., a new species of the genus Micromonospora isolated from deep-sea sediment in Japan

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