2019
DOI: 10.1128/jvi.00161-19
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Abstract: Influenza A virus matrix protein M1 is involved in multiple stages of the viral infectious cycle. Despite its functional importance, our present understanding of this essential viral protein is limited. The roles of a small subset of specific amino acids have been reported, but a more comprehensive understanding of the relationship between M1 sequence, structure, and virus fitness remains elusive. In this study, we used deep mutational scanning to measure the effect of every amino acid substitution in M1 on vi… Show more

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Cited by 27 publications
(27 citation statements)
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“…For example, some proteins may be more conserved and some less, and ( ) would be shifted towards larger and smaller s, respectively. Parameter ( ) is measured in experiment by performing site mutagenesis for each site, one by one, and evaluating fitness differences between the wild type and mutant strains (for example, by growth competition experiment) (Lee, et al 2018;Hom, et al 2019). Again, ( ) does not depend on the state of population, only on a virus and the conditions of its propagation.…”
Section: Resultsmentioning
confidence: 99%
“…For example, some proteins may be more conserved and some less, and ( ) would be shifted towards larger and smaller s, respectively. Parameter ( ) is measured in experiment by performing site mutagenesis for each site, one by one, and evaluating fitness differences between the wild type and mutant strains (for example, by growth competition experiment) (Lee, et al 2018;Hom, et al 2019). Again, ( ) does not depend on the state of population, only on a virus and the conditions of its propagation.…”
Section: Resultsmentioning
confidence: 99%
“…Here we observed a greater percentage of nonsynonymous changes in the ORFs encoding the membrane associated proteins HA, NA, M2 and NB suggesting these ORFs were more tolerant of mutation. It has been shown that different regions of the influenza A genome have a higher tolerance for mutation than others [ 56 , 57 ]. However, as most of the variants in the NA, M2 and NB ORFs did not become fixed in the quasispecies population it suggests that, in our experimental setup, antibody pressure was not the only factor driving change in these segments.…”
Section: Discussionmentioning
confidence: 99%
“…We hope that our quasi-equilibrium approach and further development of this technique will prove useful for all researchers interested in finding fitness landscapes of various organisms from their genetic samples. Also, it will be very valuable to compare these data with the result of deep mutational scanning (LEE et al 2018;HOM et al 2019), which we hope to do in the future.…”
Section: Discussionmentioning
confidence: 99%