Decreased tumor growth in Walker 256 tumor-bearing rats chronically supplemented with fish oil involves COX-2 and PGE2 reduction associated with apoptosis and increased peroxidation

Mund, Rogéria C.; Pizato, Nathalia; Bonatto, Sandro José Ribeiro; Nunes, Everson Araújo; Vicenzi, Thiago; Tanhoffer, Ricardo A.; Oliveira, Heloísa Helena Paro de; Curi, Rui; Calder, Philip C.; Fernandes, Luíz Cláudio

doi:10.1016/j.plefa.2006.11.008

Cited by 37 publications

(44 citation statements)

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“…This content is in agreement with that obtained by others [35]. Mund et al [33] have demonstrated that tumor tissue obtained from rats supplemented with fish oil, a rich source of n-3 PUFAs, had a 3-fold higher degree of lipid peroxidation when compared to those fed regular chow. Increased fatty acid peroxidation is known to produce some deleterious effects, as induction of apoptosis [33].…”

Section: Discussionsupporting

confidence: 92%

“…Another mechanism by which physical exercise and SLO supplementation can cause reduction of tumor growth seems to involve induction of apoptotic process by increasing lipid peroxidation in tumor tissue and modifying the pattern of expression of proteins linked to induction of cell death [33]. Polyunsaturated fatty acids are more prone to peroxidation than saturated or monounsaturated ones and it is known that peroxidation products may alter cellular membrane structure leading to cell death [34].…”

Section: Discussionmentioning

confidence: 99%

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Exercise and Shark Liver Oil Supplementation Reduce Tumor Growth and Cancer Cachexia in Walker 256 Tumor Bearing Rats

Bordignon¹

2014

J Cancer Sci Ther

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This study investigated whether exercise associated to shark liver oil supplementation (1 g/kg b.w./day) affects tumor growth, cachexia, lipid peroxidation and proteins expression involved in cell death in Walker 256 tumorbearing rats. Animals were divided into 4 groups: sedentary tumor-bearing (W), sedentary tumor-bearing shark liver oil supplemented (WSL), exercised tumor-bearing (EW) and exercised tumor-bearing shark liver oil supplemented (EWSL). Training sessions consisted of 6 bouts, 30 seconds each with 50% body-weight load attached to the trunk followed by 1 minute of resting (jump training). Five minutes after the finish jump training the exercise groups were subjected to 30 minutes of continuous swimming with a load equivalent to 6% of body weight, 4 times a week during 8 weeks. Tumor cells were injected at the 6 th training week and all groups were killed 15 days post inoculation. Tumor weight (g) in W group was of 26.50 ± 1.79 and in the WSL, EW and EWSL was of 14.08 ± 0.91, 15.60 ± 0.55 and 12.60 ± 1.07, respectively. The group W showed hypoglycemia (68.67 ± 2.12 mg/dl), hyperlactacidemia (1.49 ± 0.06 mmol/L), hypertriacylglycerolemia (161.4 ± 9.4 mg/dl) and body weight reduction (13.21 ± 2.25 g) characterizing cachexia state. The groups WSL, EW and EWSL presented reduction of tumor cells proliferation ex vivo, and the production of hydroperoxide and apoptosis was increased. Bax/Bcl-2 expression ratio was increased only in the exercised groups. Shark liver oil supplementation and exercise alone were able in to avoid the installation of cachexia state and also reduced tumor growth, but the association of both cause further effect only in the tumor growth.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 99%

Exercise and Shark Liver Oil Supplementation Reduce Tumor Growth and Cancer Cachexia in Walker 256 Tumor Bearing Rats

Bordignon¹

2014

J Cancer Sci Ther

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“…Due to the complexity of the mechanism activating the inflammatory pathway, several strategies have been adopted in order to block and/or minimize NF-kB activation in several diseases. 1,20,27 ACTIVATION OF THE ANTI-INFLAMMATORY PATHWAY: ROLE OF INTERLEUKIN 10 IL-10 is a 17 kDa homodymeric polypeptide which was first described as a factor produced by auxiliary lymphocyte T (type 2), with inhibiting properties upon clones of auxiliary lymphocyte T (type 1), notably on the proliferative response and production of cytokines. 28 This cytokine is produced by different types of cells, especially inflammatory cells such as macrophages and T lymphocytes, for which it is the main inhibitor of cytokine synthesis and functional activity.…”

Section: Cancer Cachexia and Cytokine Profilementioning

confidence: 99%

Regulation of inflammation in the adipose tissue in cancer cachexia: effect of exercise

Lira

Rosa

Zanchi

et al. 2009

Cell Biochemistry & Function

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The paraneoplastic syndrome of cachexia is considered a degenerative chronic inflammatory disease, being deeply related to the increase of pro-inflammatory factors, especially tumour necrosis factor alpha (TNF-a). It is known that the adipose tissue is affected by cachexia and contributing with the secretion of pro-inflammatory factors which reach the adjacent tissues and the circulation. The effect of proinflammatory factors is balanced by the effect of anti-inflammatory factors, such as interleukin 10 (IL-10). The IL-10/TNF-a ratio has been recently postulated as a marker for the assessment of the degree of inflammation, which correlates with disease-associated morbidity and mortality. In order to counteract inflammation in chronic disease, our group has currently adopted chronic endurance exercise in models of cancer cachexia and chronic heart failure. Since it is clear that white adipose tissue is strongly implicated in the secretion of both pro-and antiinflammatory factors in disease, we chose to address its contribution to cachexia-related inflammation and the effect of endurance training on the capacity of cytokine expression and secretion by this tissue. Our results show an enhancement of IL-10 adipose tissue content, and increased IL-10/TNF-a ratio induced by endurance training. The mechanisms are discussed.

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“…A suplementação com óleo de peixe (SWO) neste estudo foi capaz de induzir a produção de maiores quantidades de lipoperóxidos quando comparada à do grupo sedentário portador de tumor (SW). Este resultado corrobora com o estudo de MUND et al, 18 no qual tumores obtidos de ratos portadores do tumor de Walker 256 suplementados com óleo de peixe, rico em AGPIs n-3, apresentaram maior peroxidação lipídica do que ratos não suplementados. A relação do câncer com a taxa de peroxidação lipídica é explicada pelo fato de que as membranas das células e organelas contêm grandes quantidades de ácidos graxos poli-insaturados e a fluidez da membrana relaciona-se à presença destas cadeias insaturadas de fosfolipídios e do colesterol.…”

Section: Pesounclassified

“…O óleo de peixe, rico em ácidos graxos poli-insaturados n-3 (AGPIs n-3), tem sido demonstrado capaz de reduzir o crescimento tumoral e a caquexia 18,23 . Os mecanismos celulares e moleculares responsáveis por estes efeitos, entretanto, ainda não são completamente conhecidos.…”

Section: Introductionunclassified

Exercício de força associado a óleo de peixe reduzem massa tumoral e caquexia em ratos

Gomes

Kryczyk

Minuzzi

et al. 2014

Rev Bras Med Esporte

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RESUMOObjetivo: Investigar o efeito do treinamento de salto associado à suplementação com óleo de peixe (1g/kg peso corporal/dia) em ratos portadores do tumor de Walker 256, sobre parâmetros bioquímicos de caquexia e crescimento tumoral. Métodos: Oitenta Ratos foram divididos em sedentário sem ou com tumor (S ou SW), exercitado (EX ou EXW), suplementado com óleo de peixe (SO ou SWO) e suplementado e exercitado (EXO ou EXWO). Sessões de treinamento de salto consistiram de 10 séries com duração de 30 segundos e intervalo de 1 minuto entre cada série. Após seis semanas de treinamento, células do tumor de Walker 256 foram inoculadas e após 15 dias os animais foram mortos. Resultados: O peso médio do tumor no grupo SW foi de 25,32 g, p<0,05 vs. ao dos SWO, EXW e EXWO (~11 g). O grupo SW apresentou hipoglicemia, hiperlactatemia, hipertriacilglicerolemia e perda de peso (-7,52±3,19g) (-7.52 ± 3.19g) grupo SW presento hipoglucemia, hiperlactatemia y hipertriacilglicerolemia y reducción de peso corporal (-7,52 ± 3,19 ABSTRACT Objective: To investigate the effect of jump training associated with fish oil (FO) supplementation (1g/Kg bodyweight/day) on biochemical parameters of cachexia and tumor growth in Walker 256 tumor-bearing rats. Methods: Eighty rats were divided into sedentary non-and tumor-bearing (S and SW), exercised (EX and EXW), FO supplemented (SO and SWO), and both supplemented and exercised (EXW and EXWO). Jump training sessions consisted of 10 series of 30 seconds each, followed by 1 minute of rest. After six weeks of jump training, ascitic cells from Walker 256 tumor bearing-rat were inoculated, and after 15 days, all the animals were sacrificed. Results: The tumor mass in the SW group was 25.32 g, p<0.05 vs the SWO, EXW and EXWO groups (~11 g). The SW group presented hypoglycemia, hyperlactacidemia and hypertriacylglycerolemia and a reduction in body weight Métodos: Ochenta ratas fueron divididas en sedentarias sin y con tumor (S o SW), ejercitadas (EX o EXW), suplementadas con aceite de pescado (SO o SWO) y ejercitadas y suplementadas de forma simultánea (EXO o EXWO). Las sesiones de entrenamiento de salto consistieron en g), lo que caracteriza el estado caquéctico. La suplementación con aceite de pescado (SWO), el ejercicio (EXW) y la asociación de ambos (EXWO) impidieron la instalación de la caquexia (p < 0,05 vs. SW). En el grupo SWO, EXW e sus asociaciones (EXWO) promovieron aumento de peso (p < 0,05 vs. SW), pero inferior al de la suplementación aislada (p < 0,05 vs. SWO). La proliferación in vitro de las células tumorales fue menor en el grupo SWO (p < 0,05 vs. SW) y el ejercicio la redujo todavía más (p < 0,05 vs. SW e SWO), no habiendo incremento cuando se asociaran ambas las terapias. La lipoperoxidación fue mayor en los grupos SWO, EXW, EXWO con respecto al grupo S (p < 0,05). La expresión de INTRODUÇÃOAlguns pacientes com câncer podem desenvolver a síndrome metabólica complexa denominada caquexia, caracterizada por progressiva perda de peso, anorexia, astenia, anemia, náusea crônica, imu...

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Decreased tumor growth in Walker 256 tumor-bearing rats chronically supplemented with fish oil involves COX-2 and PGE2 reduction associated with apoptosis and increased peroxidation

Cited by 37 publications

References 33 publications

Exercise and Shark Liver Oil Supplementation Reduce Tumor Growth and Cancer Cachexia in Walker 256 Tumor Bearing Rats

Exercise and Shark Liver Oil Supplementation Reduce Tumor Growth and Cancer Cachexia in Walker 256 Tumor Bearing Rats

Regulation of inflammation in the adipose tissue in cancer cachexia: effect of exercise

Exercício de força associado a óleo de peixe reduzem massa tumoral e caquexia em ratos

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