Decreased peripheral blood mitochondrial DNA content is related to HbA<sub>1c</sub>, fasting plasma glucose level and age of onset in Type 2 diabetes mellitus

Xu, Fei; Zhou, Xin; Shen, Fan; Pang, Roberta; Liu, S. M.

doi:10.1111/j.1464-5491.2011.03565.x

Cited by 45 publications

(53 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In this cross-sectional study, we have used a stepwise multiple regression analysis, adjusting for potential confounders such as age,15) BMI,16) and various cardio-metabolic parameters,1718) to show that postmenopausal women who partake in regular exercise have a significantly higher leukocyte mtDNA copy number than postmenopausal women who do not exercise.…”

Section: Discussionmentioning

confidence: 99%

“…An independent association between leukocyte mtDNA copy number and exercise was confirmed using a stepwise multiple linear regression model with a significance threshold of 0.15. Potential confounding variables were defined as those that were significantly associated (P<0.1) with leukocyte mtDNA copy number using Pearson's correlation analysis, either in this study (triglyceride, WHR, waist circumference, and homocysteine level), or in previously reported studies (age,15) BMI,16) systolic and diastolic blood pressure, HOMA-IR, and levels of triglyceride and HDL cholesterol1718)). Multicollinearity between independent variables was evaluated using Pearson's correlation coefficients.…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Association between Leukocyte Mitochondrial DNA Copy Number and Regular Exercise in Postmenopausal Women

et al. 2016

View full text Add to dashboard Cite

BackgroundPrevious studies suggest that habitual exercise can improve skeletal mitochondrial function; however, to date, the association between exercise and mitochondrial function in peripheral leukocytes has not been reported. The aim of this study was to evaluate the relationship between regular exercise and mitochondrial function by measuring leukocyte mitochondrial DNA (mtDNA) copy number in postmenopausal women.MethodsThis cross-sectional study included 144 relatively healthy, non-diabetic, non-smoking, postmenopausal women. Clinical parameters, including anthropometric measurements and cardio-metabolic parameters, were assessed. Regular exercise was defined as at least 150 minutes per week of moderate-intensity activity, or an equivalent combination of moderate and vigorous-intensity activity, over a duration of at least 6 months. Leukocyte mtDNA copy numbers were measured using real-time polymerase chain reaction assays, and these were normalized to the β-globin copy number to give the relative mtDNA copy number.ResultsThe mtDNA copy number of peripheral leukocytes was significantly greater in the exercise group (1.33±0.02) than in the no exercise group (1.05±0.02, P<0.01). Stepwise multiple regression analysis showed that regular exercise was independently associated with mtDNA copy number (β=0.25, P<0.01) after adjusting for the variables age, body mass index, waist-to-hip ratio, systolic and diastolic blood pressure, homeostasis model assessment of insulin resistance value, and levels of high-density lipoprotein cholesterol, triglycerides, and homocysteine.ConclusionRegular exercise is associated with greater leukocyte mtDNA copy number in postmenopausal women.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Association between Leukocyte Mitochondrial DNA Copy Number and Regular Exercise in Postmenopausal Women

et al. 2016

View full text Add to dashboard Cite

show abstract

“…An African-American study demonstrated epigenetic factors similar to psychological stress and social context that are correlated with swelling and infection in heart diseases and strokes [5]. Previous studies validated the contribution of epigenomics in the therapeutics of breast cancer [6] in which it was observed that several dietary chemo-preventive agents (retinoids/Vitamin A, green tea, Vitamin D, etc.) acted on the miRNA signaling pathways, so as to obstruct the uncontrolled metabolic mechanisms of breast cancer.…”

Section: Introductionmentioning

confidence: 99%

Genetic and epigenetic modifications in the pathogenesis of diabetic retinopathy: a molecular link to regulate gene expression

Pradhan¹,

Upadhyay²,

Tiwari³

et al. 2016

New Front Ophthalmol

View full text Add to dashboard Cite

Intensification in the frequency of diabetes and the associated vascular complications has been a root cause of blindness and visual impairment worldwide. One such vascular complication which has been the prominent cause of blindness; retinal vasculature, neuronal and glial abnormalities is diabetic retinopathy (DR), a chronic complicated outcome of Type 1 and Type 2 diabetes. It has also become clear that “genetic” variations in population alone can’t explain the development and progression of diabetes and its complications including DR. DR experiences engagement of foremost mediators of diabetes such as hyperglycemia, oxidant stress, and inflammatory factors that lead to the dysregulation of “epigenetic” mechanisms involving histone acetylation and histone and DNA methylation, chromatin remodeling and expression of a complex set of stress-regulated and disease-associated genes. In addition, both elevated glucose concentration and insulin resistance leave a robust effect on epigenetic reprogramming of the endothelial cells too, since endothelium associated with the eye aids in maintaining the vascular homeostasis. Furthermore, several studies conducted on the disease suggest that the modifications of the epigenome might be the fundamental mechanism(s) for the proposed metabolic memory’ resulting into prolonged gene expression for inflammation and cellular dysfunction even after attaining the glycemic control in diabetics. Henceforth, the present review focuses on the aspects of genetic and epigenetic alterations in genes such as vascular endothelial growth factor and aldose reductase considered being associated with DR. In addition, we discuss briefly the role of the thioredoxin-interacting protein TXNIP, which is strongly induced by high glucose and diabetes, in cellular oxidative stress and mitochondrial dysfunction potentially leading to chromatin remodeling and ocular complications of diabetes. The identification of disease-associated genes and their epigenetic regulations will lead to potential new drugs and gene therapies as well as personalized medicine to prevent or slow down the progression of DR.

show abstract

“…In addition, we added baseline eGFR to Models 3 and 4. Because lower mtDNA copy number measured from peripheral blood has been associated with diabetes, one of the strongest risk factors of incident CKD, 42,43 we tested for the interaction between mtDNA copy number and prevalent diabetes on the basis of Model 4. We also conducted sensitivity analyses on the basis of Model 4.…”

Section: Statistical Analysesmentioning

confidence: 99%

Association between Mitochondrial DNA Copy Number in Peripheral Blood and Incident CKD in the Atherosclerosis Risk in Communities Study

Tin

Grams²,

Ashar

et al. 2016

JASN

118

115

View full text Add to dashboard Cite

Mitochondrial dysfunction in kidney cells has been implicated in the pathogenesis of CKD. Mitochondrial DNA (mtDNA) copy number is a surrogate measure of mitochondrial function, and higher mtDNA copy number in peripheral blood has been associated with lower risk of two important risk factors for CKD progression, diabetes and microalbuminuria. We evaluated whether mtDNA copy number in peripheral blood associates with incident CKD in a population-based cohort of middle-aged adults. We estimated mtDNA copy number using 25 high-quality mitochondrial single nucleotide polymorphisms from the Affymetrix 6.0 array. Among 9058 participants, those with higher mtDNA copy number had a lower rate of prevalent diabetes and lower C-reactive protein levels and white blood cell counts. Baseline eGFR did not differ significantly by mtDNA copy number. Over a median follow-up of 19.6 years, 1490 participants developed CKD. Higher mtDNA copy number associated with lower risk of incident CKD (highest versus lowest quartile: hazard ratio 0.65; 95% confidence interval, 0.56 to 0.75; P,0.001) after adjusting for age, sex, and race. After adjusting for additional risk factors of CKD, including prevalent diabetes, hypertension, C-reactive protein level, and white blood cell count, this association remained significant (highest versus lowest quartile: hazard ratio 0.75; 95% confidence interval, 0.64 to 0.87; P,0.001). In conclusion, higher mtDNA copy number associated with lower incidence of CKD independent of traditional risk factors and inflammation biomarker levels in this cohort. Further research on modifiable factors influencing mtDNA copy number may lead to improvement in the prevention and treatment of CKD.

show abstract

Decreased peripheral blood mitochondrial DNA content is related to HbA_1c, fasting plasma glucose level and age of onset in Type 2 diabetes mellitus

Abstract: Our results demonstrate that lower peripheral blood mtDNA content is associated with Type 2 diabetes in Chinese individuals, and HbA(1c), fasting plasma glucose and age of onset are the major factors affecting the mtDNA content.

Cited by 45 publications

References 31 publications

Association between Leukocyte Mitochondrial DNA Copy Number and Regular Exercise in Postmenopausal Women

Association between Leukocyte Mitochondrial DNA Copy Number and Regular Exercise in Postmenopausal Women

Genetic and epigenetic modifications in the pathogenesis of diabetic retinopathy: a molecular link to regulate gene expression

Association between Mitochondrial DNA Copy Number in Peripheral Blood and Incident CKD in the Atherosclerosis Risk in Communities Study

Contact Info

Product

Resources

About

Decreased peripheral blood mitochondrial DNA content is related to HbA1c, fasting plasma glucose level and age of onset in Type 2 diabetes mellitus

Abstract: Our results demonstrate that lower peripheral blood mtDNA content is associated with Type 2 diabetes in Chinese individuals, and HbA(1c), fasting plasma glucose and age of onset are the major factors affecting the mtDNA content.

Cited by 45 publications

References 31 publications

Association between Leukocyte Mitochondrial DNA Copy Number and Regular Exercise in Postmenopausal Women

Association between Leukocyte Mitochondrial DNA Copy Number and Regular Exercise in Postmenopausal Women

Genetic and epigenetic modifications in the pathogenesis of diabetic retinopathy: a molecular link to regulate gene expression

Association between Mitochondrial DNA Copy Number in Peripheral Blood and Incident CKD in the Atherosclerosis Risk in Communities Study

Contact Info

Product

Resources

About

Decreased peripheral blood mitochondrial DNA content is related to HbA_1c, fasting plasma glucose level and age of onset in Type 2 diabetes mellitus