Decreased fertilization rate and embryo quality after ICSI in oligozoospermic men with microdeletions in the azoospermia factor c region of the Y chromosome

Golde, Ron J T van; Wetzels, Alex M.M.; Graaf, Robin de; Tuerlings, J.H.A.M.; Braat, D.D.M.; Kremer, J.A.M.

doi:10.1093/humrep/16.2.289

Cited by 95 publications

(83 citation statements)

References 26 publications

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“…Our study shows that Y chromosome AZFc microdeletion group has a reduced fertilization rate and an increased cleaved embryo rate compared with men without microdeletion and the difference is statistically significant. The reduced fertilization rate is consistent with the result reported by van Golde RJ et al [9]. This result suggests that Y chromosome AZFc microdeletion would affect the fertilization rate of ICSI treatment, leading to reduced fertilizing ability of sperm, and therefore an inability to activate oocytes.…”

Section: Discussionsupporting

confidence: 91%

“…Our results confirmed the results of previous studies [24,25] in a larger cohort. Recently, several investigators have shown that embryo characteristics following ICSI using sperm obtained from men with Y chromosome microdeletions were not adversely affected by the deletion [4,9,10,26,27]. ICSI brings new hope for severe oligozoospermic men who wish to conceive children with their own gametes [28].…”

Section: Discussionmentioning

confidence: 99%

“…1). The AZF deletions are known to be associated with various spermatogenetic alterations including Sertoli cell-only syndrome (SCOS), maturation arrest, and hypospermatogenesis [9,10]. The development of intracytoplasmic sperm injection (ICSI) has allowed patients with severe oligozoospermia to have children.…”

Section: Introductionmentioning

confidence: 99%

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Y chromosome AZFc microdeletion may not affect the outcomes of ICSI for infertile males with fresh ejaculated sperm

Liu

Qiao

et al. 2013

J Assist Reprod Genet

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Purpose To explore whether the presence of a Y chromosome AZFc microdeletion confers any adverse effect on the outcomes of intracytoplasmic sperm injection (ICSI) with fresh ejaculated sperm. Methods A total of 143 oligozoospermia patients with Y chromosome AZFc microdeletion in ICSI cycles in a fiveyear period were studied. Infertile men with normal Y chromosome in ICSI at the same time-frame were used as controls matched to the study group for age of female, female's body mass index, male's age, infertility duration and number of oocytes retrieved. Retrospective case-control study was used. Results There were no significant differences between groups in clinical outcomes of endometrial thickness, transferred embryos, good embryo rates, implantation rates, biochemical pregnancy rates, clinical pregnancy rates, ectopic pregnancy rates, miscarriage rates, preterm birth rates, the ratio of male and female babies, newborn body height, newborn weight, low birth weight and birth defects (P>0.05). Patients with Y chromosome AZFc microdeletion had a lower fertilization rate (61.8 % vs. 67.8 %, P<0.05) and higher cleaved embryo rate (94.0 % vs. 88.1 %, P<0.05). Conclusions ICSI clinical outcomes for oligozoospermic patients with Y chromosome AZFc microdeletion are basically comparable to that of infertile patients with normal Y chromosomes. The results of ICSI were not affected by the AZFc deletion. Preimplantation genetic diagnosis (PGD) before ICSI for Y chromosome AZFc microdeletion may not be a justifiable regular procedure if the couples didn't care the vertical transmission of Y chromosome deletion.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 99%

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Y chromosome AZFc microdeletion may not affect the outcomes of ICSI for infertile males with fresh ejaculated sperm

Liu

Qiao

et al. 2013

J Assist Reprod Genet

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“…Also, men with Yq microdeletions have poor embryo quality and lower success rates of ICSI as compared to non-deleted counterparts [5,39]. However, the contribution of AZFc subdeletions on outcome of assisted reproduction is unknown.…”

Section: Discussionmentioning

confidence: 99%

“…Since men with deletions of entire AZFc have poor outcome after assisted reproduction [5,39], we also investigated if the AZFc subdeletions have any influence on fertilization rate, embryo transfer rate, embryo quality and pregnancy outcome in male partners of couples undergoing ICSI (intracytoplasmic sperm injection).…”

Section: Introductionmentioning

confidence: 99%

Susceptibility of gr/gr rearrangements to azoospermia or oligozoospermia is dependent on DAZ and CDY1 gene copy deletions

Sen

Ambulkar

Hinduja

et al. 2015

J Assist Reprod Genet

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Purpose The purpose of this study was to determine the association of AZFc subdeletions (gr/gr, b1/b3 and b2/b3) and deletion of DAZ and CDY1 gene copies with male infertility Methods Three hundred twelve controls, 172 azoospermic and 343 oligozoospermic subjects were subjected to AZFc subdeletion typing by STS PCR. Deletion of DAZ and CDY1 gene copies was done using sequence family variant analysis. Sperm concentration and motility were compared between men with and without AZFc subdeletions. Effect of the AZFc subdeletions on ICSI outcome was evaluated. Results Amongst the three AZFc subdeletions, the frequency of gr/gr was higher in oligozoospermic (10.5 %) and azoospermic (11.6 %) men as compared to controls (5.1 %). In men with AZFc subdeltions, loss of two DAZ and one CDY1 gene copy made them highly susceptible to azoospermia and severe oligozoospermia with OR of 29.7 and 26, respectively. These subdeletions had no effect on ICSI outcome, albeit there were an increased number of poor quality embryos in AZFc subdeleted group. Conclusion AZFc subdeletions are a major risk factor for male infertility in the Indian population. In the subjects with AZFc subdeletions, the deletion of DAZ and CDY1 gene copies increases its susceptibility to azoospermia or severe oligozoospermia. Since these deletions can be vertically transmitted to the future male offspring by ICSI, it will be essential to counsel the couples for the transmission of the genetic defect in the male offspring born after assisted reproduction and the risk of perpetuating infertility in future generation.

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Induction of the Acrosome Reaction and Zona‐Free Hamster Oocyte Penetration by a Bull With Complete Teratospermia Versus a Half Brother With Normal Sperm

Hough

Kaproth

Foote

2002

Journal of Andrology

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A fertile bull producing normal sperm and a sterile half brother exhibiting 100% teratospermia were available to study an induced sperm acrosome reaction and oocyte penetration. Pedigree analysis indicated that this condition was inherited. Experiments were undertaken to study the induction of the acrosome reaction using dilaurylphosphatidylcholine (PC12) liposomes, because this procedure was previously established to be highly correlated with bull fertility. The sperm from each bull were incubated with several PC12 concentrations for varying time periods. The initial percentages of sperm from the sterile bull with intact, partially intact, and lost acrosomes were 67%, 18%, and 14%, respectively, vs 82%, 13%, and 5% for the fertile bull (P Ͻ .05). After incubation for 15 minutes with 50 M PC12 liposomes the corresponding values were, respectively, 51%, 26%, and 19%; and 60%, 28%, and 12%. Thus, the differences after induction of the acrosome reaction, although significant (P Ͻ .05), were small. The number of sperm adhered to each oocyte averaged 22 and 10, respectively, for the fertile and sterile bulls, whereas 74% of the fertile bull sperm and only 11% of the sterile bull sperm penetrated oocytes. Mixing the sperm-oocyte complex during incubation and increasing the sperm concentration during incubation to compensate for differences in sperm motility did not markedly affect oocyte penetration by teratogenic sperm, which is consistent with this bull being sterile. In other studies, microinjection of this type of sperm was demonstrated to induce fertilization, so the consequences of using sperm with hereditary defects in assisted reproductive programs to overcome human male sterility may be a concern.

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Decreased fertilization rate and embryo quality after ICSI in oligozoospermic men with microdeletions in the azoospermia factor c region of the Y chromosome

Cited by 95 publications

References 26 publications

Y chromosome AZFc microdeletion may not affect the outcomes of ICSI for infertile males with fresh ejaculated sperm

Y chromosome AZFc microdeletion may not affect the outcomes of ICSI for infertile males with fresh ejaculated sperm

Susceptibility of gr/gr rearrangements to azoospermia or oligozoospermia is dependent on DAZ and CDY1 gene copy deletions

Induction of the Acrosome Reaction and Zona‐Free Hamster Oocyte Penetration by a Bull With Complete Teratospermia Versus a Half Brother With Normal Sperm

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