Decreased expression of Small glutamine-rich tetratricopeptide repeat-containing protein (SGT) correlated with prognosis of Hepatocellular carcinoma

Abstract

Small glutamine-rich tetratricopeptide repeat-containing protein (SGT) is an ubiquitously expressed cochaperone of heat shock cognate protein of 70 kDa (Hsc70). SGT binds to the C terminus of Hsc70 to recruit Hsc70 into complexes of diverse function. SGTB was identified as an isoform of SGT with 60% amino acid sequence homology. To investigate the expression of SGTB in hepatocellular carcinoma (HCC) and determine its correlation with tumor progression and prognosis, we evaluated the expression levels of SGTB in HCCs and corresponding adjacent non-tumor liver tissues. We also assessed the association between their expression and clinicopathologic parameters. The expression of SGTB was absent or low in HCCs while it was notable in paracancerous tissues from 108 patients by western blotting and immunochemistry (P < 0.05). Among the 108 HCCs, low expression of SGTB was associated with gender, histological grade (P<0.001) and HBsAg expression (P=0.002). Univariate analysis showed that the low SGTB expression was associated with poor prognosis (P<0.001). Thus, decreased expression of SGTB may be a favorable independent poor prognostic parameter for hepatocellular carcinoma.

Bioinformatics identification of miR‐514b‐5p promotes NSCLC progression and induces PI3K/AKT and p38 pathways by targeting small glutamine‐rich tetratricopeptide repeat‐containing protein beta

“…Small glutamine-rich tetratricopeptide repeat-containing protein (SGT), including SGTA and SGTB, was first identified as a protein interacting with HIV type 1 and parvovirus H-1 [23] and is an ubiquitously expressed molecular chaperone of Hsc70 [24]. SGTB was found to be dramatically decreased in HCC tissues, and low expression level of SGTB was shown to be associated with histological grade, HBsAg expression and a poor prognosis [25]. Tian et al [47] found that miR-365b functioned as a cancer-promoting gene and promoted hepatocellular carcinoma motility and spreading by suppressing SGTB expression.…”

“…SGT has two forms: SGT alpha (SGTA) and SGT beta (SGTB) [24]. SGTB was found to be dramatically decreased in hepatocellular carcinoma (HCC) tissues compared to normal liver tissues, and downregulation of SGTB was shown to be related to histological classification, HBsAg expression and a poor prognosis [25]. Here, we found that miR-514b-5p was dramatically upregulated in NSCLC tissues, and miR-514b-5p expression was positively associated with poor outcomes.…”

The Histone Variant MacroH2A1 Impacts Circadian Gene Expression and Cell Phenotype in an In Vitro Model of Hepatocellular Carcinoma

“…The protein encoded by ABCG5 (fold change 16.35) is one of the ATP-binding cassette (ABC) transporters superfamily members, involved in extra and intracellular membrane transport of different molecules and, in particular, of neutral sterol in hepatobiliary and transintestinal cholesterol excretion and its inactivating mutations cause a rare genetic disorder, Sitosterolemia [44]. SGTB (fold change 3.09) encodes small glutamine-rich tetratricopeptide repeat containing beta, an isoform of SGT, a co-chaperone of heat shock cognate protein of 70 kDa (Hsc70), and its down-regulation by miR-365b enhances HCC cell migration and invasion in vitro and low SGTB tissue expression was found correlated with more favorable HCC progression and prognosis [45,46]. The protein-coding gene SER-PINH1 (fold change 2.80) encodes a member of the serine protease inhibitor superfamily.…”