Decrease of [<sup>3</sup>H]5‐HT high affinity binding and 5‐HT adenylate cyclase activation after kainic acid lesion in rat brain striatum

Fillion, G.; Beaudoin, Dominique; Rousselle, Jean Claude; Deniau, Jean-Michel; Fillion, Marie‐Paule; Dray, F.; Jacob, J.

doi:10.1111/j.1471-4159.1979.tb05189.x

Cited by 52 publications

(14 citation statements)

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“…5-HT2 binding sites have micromolar affinity for 5-HT and nanomolar affinity for spiperone and ketanserin. Recent evidence from our laboratory (3) and from Fillion et al (4) 3 and 4; un-published data). In an effort to maintain consistency in nomenclature, we will refer to this last site as a 5-HT3 type.…”

mentioning

confidence: 94%

Demonstration of inter- and intraspecies differences in serotonin binding sites by antibodies from an autistic child.

Todd¹,

Ciaranello²

1985

Proc. Natl. Acad. Sci. U.S.A.

124

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Serotonin (5-HT) binding sites from bovine and rat cerebral cortex membranes share pharmacological properties that allow both to be subclassified by the same criteria. We show here that [3Hj5-HT binding sites from human cortex also possess pharmacological properties that follow the same subclassification scheme as for bovine and rat cortex. In addition, we show that solubilized 5-HT1 and 5-HT3 sites from all three species have an s20,w value of 3.4. Despite these similar pharmacological and physical characteristics, we can demonstrate antigenic differences between receptor types and spe- (refs. 3 and 4; unpublished data). In an effort to maintain consistency in nomenclature, we will refer to this last site as a 5-HT3 type. We have also solubilized the 5-HT1 and 5-HT3 sites from bovine cortex and described their biophysical characteristics (3).In the course of a study on hyperserotonemia in autistic children, we found a child with circulating antibodies directed against human cortical 5-HT binding sites. Further investigation disclosed that these antibodies cross-reacted only with the 5-HT1A subclass of 5-HT1 binding sites. This gave us the opportunity to study antigenic properties ofthe 5-HT1A class of binding sites across species. In this report, we compare the properties of 5-HT1 and 5-HT3 sites from human, rat, and bovine cortex, describe the characterization of the antibodies from the autistic child, and demonstrate intra-and interspecies differences in antigenicity in 5-HT binding sites. MATERIALS AND METHODSThe child reported here is a 9-year-old girl who was in good health and free of all medications at the time of the study. She met DSM-III (5) criteria for infantile autism, as determined by three independent, experienced clinical evaluators. Venous blood samples were collected into EDTA-containing tubes. Platelet 5-HT as measured by the method of Stahl et al. (6) was 72 ng per 108 platelets or 550 ng/ml of whole blood. IgG class antibodies were purified from serum samples by protein A-Sepharose 4B (Sigma) affinity chromatography (7). Greater than 85% of serum IgG was recovered in one column passage. Human frontal cortex tissue was obtained at autopsy. Membranes from human, bovine, or rat cortex were prepared as described (3). Gel electrophoresis was performed on 10% acrylamide/0.1% sodium dodecyl sulfate slab gels. Coomassie blue staining and densitometry were carried out as described (8). High molecular weight standards (Sigma) were electrophoresed for molecular weight determinations. The agarose IgG fraction of goat antihuman IgG was obtained from Miles-Yeda (Rehovot, Israel).Samples were analyzed for [3H]5-HT binding by equilibrium saturation (Scatchard) analysis (9) as described (3), except as follows: membrane samples were incubated with and without purified antibodies for 20 min at 30°C and then placed on ice for 2 hr. The equivalent serum dilution was 1:3 in all cases.[3H]5-HT (0.5-200 nM, 28 Ci/mmol; 1 Ci = 37GBq; New England Nuclear) and 10 AM pargyline were then added to aliquots of m...

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confidence: 94%

Demonstration of inter- and intraspecies differences in serotonin binding sites by antibodies from an autistic child.

Todd¹,

Ciaranello²

1985

Proc. Natl. Acad. Sci. U.S.A.

124

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“…In addition, adenylate cyclase in free cell preparation from brain tissue (18,19) and in membrane preparations from hippocampus (20)(21)(22)(23) was found to respond to serotonin. Lesions of serotonergic afférents to the hippocampus suggest that the serotonin-stimulated adenylate cyclase in the hippocampus is located on the postsynaptic site of the serotonergic system (23,24). The receptors involved showed a high apparent affinity and have been classified as the 5-HT1 subtype (22,25).…”

Section: Discussionmentioning

confidence: 99%

Intraventricular injection of agents that enhance cyclic adenosine monophosphate formation leads to inhibition of proestrous luteinizing hormone surge in rats

Taleisnik

Haymal²,

Caligaris³

1993

Acta Endocrinologica

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Taleisnik S, Haymal B, Caligaris L. Intraventricular injection of agents that enhance cyclic adenosine monophosphate formation leads to inhibition of proestrous luteinizing hormone surge in rats. Acta Endocrinol 1993:129:273-8. The effect of increasing hypothalamic levels of 3\m=' \,5\m=' \-cyclicadenosine monophosphate (cAMP) on the preovulatory surge of luteinizing hormone (LH) and ovulation was studied in cycling rats. Animals bearing chronically implanted guiding cannulae into the third ventricle were injected with agents known to enhance the cellular levels of cAMP. Hourly blood samples from the unanesthetized, unrestrained rats were obtained between 11.00 and 1 7.00 h through a plastic cannula inserted into the jugular vein. Intraventricular injections of serotonin (7.5 mg/ml; 2 \g=m\l ) in the morning of proestrous blocked the preovulatory surge of LH and ovulation. This effect was assigned to an increased neuronal level of cAMP because it was prevented by a serum anti-cAMP. Third-ventricle injections of 2 \ g=m\ l of forskolin (0.5 mmol/l), guanosine 5\m='\-0-(3-thiotriphosphate)(2 mmol/l) or dibutyryl-cAMP (1 mmol/l) at 11.00 h on the day of proestrus mimicked the inhibitory effect of serotonin on the proestrous release of LH. It is suggested that serotonin inhibits LH surge by acting directly on LH-releasing hormone neurons and/or on neurons that provide inputs to these neurons involving cAMP as a second messenger. Neurons releasing \g=g\-aminobutyricacid (GABA) may serve as interneurons sensitive to serotonin, as well as to cAMP, inasmuch as the inhibitory effect of forskolin on the release of LH was partially blocked by the GABA antagonists, picrotoxin and bicuculline.Regulation of gonadotropin secretion depends directly on the secretion of gonadotropin-releasing hormone (GnRH). The neurons that elaborate and secrete this hormone form a continuum from the telencephalic diagonal band of Broca and the diencephalic areas of the periventricular, preoptic and anterior hypothalamic areas. These neurons constitute a final common path¬ way of which the most prominent projection is to the median eminence (1). Inputs to the GnRH neurons play a fundamental role in controlling the secretion of the products of these neurons. There is evidence for synaptic associations between GnRH neurons and nerve endings containing dopamine /Miydroxylase (2), tyrosine hydroxylase (2-4), y-aminobutyric acid decarboxylase (2, 5), neurotensin and substance P (6, 7), serotonin (8), opioids (9, 10) and neuropeptide Y (11). In addition, a variety of neurotransmitters have been demonstrated to influence the secretion of luteinizing hormone (LH) (12).The generation of cyclic adenosine 3',5'-monophosphate (cAMP) is an obligatory step for the action of many neurotransmitters after their binding to specific recep¬ tors on the surface of the target cells. The accumulation of cAMP in GnRH neurons may therefore be a possible process in the action of neurotransmitters affecting LH secretion.The aim of this study was to explore whether changes...

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“…It has been shown that these sites are neur onal and postsynaptically located [8]. The re cognition sites exist under two conformation al structures which interconvert and corre spond to a binding of 3H-5-HT with a high (Kd=30 nM) or a low affinity constant (Kd =20 nM), respectively [11].…”

Section: Discussionmentioning

confidence: 99%

“…It has been previously demonstrated that the observed binding sites for 3H-5-HT corre sponded to postsynaptosomal receptors; in deed, they disappeared after lesion of postsynaptic neurons [8] whereas degeneration of change (presynaptic) serotoninergic neurons in adult rat brain induced no [7] or even an in crease [16] of the binding. The latter observa tions indicate also that the studied 3H-5-HT binding do not correspond to the uptake sites which are located presynaptically.…”

Section: Discussionmentioning

confidence: 99%

“…It has been shown previously that mam malian brain contains specific recognition sites for 3H-5-HT, binding this amine with a high affinity constant (KD in the nanomolar range) [see review by Fillion,5], This popula tion of sites is neuronal and more precisely postsynaptically located [8], The physiologi cal effector presumably coupled to this recog nition site is an adenylate cyclase sensitive to 5-HT with a high affinity constant (Kacl= l nM) [3,4] and also located postsynaptically [8], It has to be noted that this population of receptors is distinct from that located on glial cells which has different binding characteris tics and different adenylate cyclase activities [10]. They are also clearly distinct from those binding 3H-spiroperidol described as 5-HT2 receptors by Peroutka et al [9].…”

Section: Introductionmentioning

confidence: 99%

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Postnatal Development of High Affinity Neuronal Recognition Sites for <sup>3</sup>H-5-HT in Rat Brain

Fillion¹,

Hernández²,

Bauguen³

et al. 1982

Dev Neurosci

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The postnatal development of ³H-5-HT specific binding sites has been studied in rat brain. Experimental conditions allowed the characterization of the class of sites corresponding to the synaptosomal receptor for 5-HT. The related affinity constant and maximal binding were measured at various ages after birth. During the second week after birth, the serotonin receptor system developed explosively, the maximal number of sites increasing from a nonsignificant value to that observed in adult rats. Lesion of the presynaptic neurons using 5,6-DHT led to a significant increase of the number of sites.

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Decrease of [³H]5‐HT high affinity binding and 5‐HT adenylate cyclase activation after kainic acid lesion in rat brain striatum

Cited by 52 publications

References 6 publications

Demonstration of inter- and intraspecies differences in serotonin binding sites by antibodies from an autistic child.

Demonstration of inter- and intraspecies differences in serotonin binding sites by antibodies from an autistic child.

Intraventricular injection of agents that enhance cyclic adenosine monophosphate formation leads to inhibition of proestrous luteinizing hormone surge in rats

Postnatal Development of High Affinity Neuronal Recognition Sites for <sup>3</sup>H-5-HT in Rat Brain

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Decrease of [3H]5‐HT high affinity binding and 5‐HT adenylate cyclase activation after kainic acid lesion in rat brain striatum

Cited by 52 publications

References 6 publications

Demonstration of inter- and intraspecies differences in serotonin binding sites by antibodies from an autistic child.

Demonstration of inter- and intraspecies differences in serotonin binding sites by antibodies from an autistic child.

Intraventricular injection of agents that enhance cyclic adenosine monophosphate formation leads to inhibition of proestrous luteinizing hormone surge in rats

Postnatal Development of High Affinity Neuronal Recognition Sites for <sup>3</sup>H-5-HT in Rat Brain

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Decrease of [³H]5‐HT high affinity binding and 5‐HT adenylate cyclase activation after kainic acid lesion in rat brain striatum