Decoupling the Direct and Indirect Biological Effects of ZnO Nanoparticles Using a Communicative Dual Cell‐Type Tissue Construct

Chia, Sing Ling; Tay, Chor Yong; Setyawati, Magdiel Inggrid; Leong, David Tai

doi:10.1002/smll.201502306

Cited by 27 publications

(16 citation statements)

References 45 publications

(64 reference statements)

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“…The serum in the medium is known to rapidly bind to the NPs and form a protein corona around the NPs 5 36 . This protein corona further helps the NPs to disperse by providing steric hindrance and electrostatic repulsion between NPs 37 38 . The absorption of protein on the NP surface not only affects the size and physical properties of NP but also affects the interaction of NPs with cellular systems.…”

Section: Resultsmentioning

confidence: 99%

Role of Zn doping in oxidative stress mediated cytotoxicity of TiO2 nanoparticles in human breast cancer MCF-7 cells

Ahamed

Khan

Akhtar

et al. 2016

Sci Rep

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We investigated the effect of Zn-doping on structural and optical properties as well as cellular response of TiO2 nanoparticles (NPs) in human breast cancer MCF-7 cells. A library of Zn-doped (1–10 at wt%) TiO2 NPs was prepared. Characterization data indicated that dopant Zn was incorporated into the lattice of host TiO2. The average particle size of TiO2 NPs was decreases (38 to 28 nm) while the band gap energy was increases (3.35 eV–3.85 eV) with increasing the amount of Zn-doping. Cellular data demonstrated that Zn-doped TiO2 NPs induced cytotoxicity (cell viability reduction, membrane damage and cell cycle arrest) and oxidative stress (reactive oxygen species generation & glutathione depletion) in MCF-7 cells and toxic intensity was increases with increasing the concentration of Zn-doping. Molecular data revealed that Zn-doped TiO2 NPs induced the down-regulation of super oxide dismutase gene while the up-regulation of heme oxygenase-1 gene in MCF-7 cells. Cytotoxicity induced by Zn-doped TiO2 NPs was efficiently prevented by N-acetyl-cysteine suggesting that oxidative stress might be the primarily cause of toxicity. In conclusion, our data indicated that Zn-doping decreases the particle size and increases the band gap energy as well the oxidative stress-mediated toxicity of TiO2 NPs in MCF-7 cells.

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Section: Resultsmentioning

confidence: 99%

Role of Zn doping in oxidative stress mediated cytotoxicity of TiO2 nanoparticles in human breast cancer MCF-7 cells

Ahamed

Khan

Akhtar

et al. 2016

Sci Rep

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“…Strategies have further been investigated to integrate blood vessels into engineered tissues including the myocardium [28–31]. For example, Leong and colleagues have provided a general and versatile strategy by using transwell-mediated layering of endothelial cells and tissue cells for drug testing [30,31]. However, generating volumetric cardiac tissues containing embedded endothelial networks remains challenging.…”

Section: Introductionmentioning

confidence: 99%

Bioprinting 3D microfibrous scaffolds for engineering endothelialized myocardium and heart-on-a-chip

et al. 2016

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Engineering cardiac tissues and organ models remains a great challenge due to the hierarchical structure of the native myocardium. The need of integrating blood vessels brings additional complexity, limiting the available approaches that are suitable to produce integrated cardiovascular organoids. In this work we propose a novel hybrid strategy based on 3D bioprinting, to fabricate endothelialized myocardium. Enabled by the use of our composite bioink, endothelial cells directly bioprinted within microfibrous hydrogel scaffolds gradually migrated towards the peripheries of the microfibers to form a layer of confluent endothelium. Together with controlled anisotropy, this 3D endothelial bed was then seeded with cardiomyocytes to generate aligned myocardium capable of spontaneous and synchronous contraction. We further embedded the organoids into a specially designed microfluidic perfusion bioreactor to complete the endothelialized-myocardium-on-a-chip platform for cardiovascular toxicity evaluation. Finally, we demonstrated that such a technique could be translated to human cardiomyocytes derived from induced pluripotent stem cells to construct endothelialized human myocardium. We believe that our method for generation of endothelialized organoids fabricated through an innovative 3D bioprinting technology may find widespread applications in regenerative medicine, drug screening, and potentially disease modeling.

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“…These results indicated that hybrid materials of chitosan–gelatine–agarose with halloysite nanotubes may be ideal scaffolds for tissue engineering applications. The tissue‐engineered constructs can be more realistic models for testing bionanomaterials …”

Section: From Earth: Clay‐based Hybrid Nanoarchitectonicsmentioning

confidence: 99%

Nanoarchitectonics for Hybrid and Related Materials for Bio‐Oriented Applications

Ariga

Leong

Mori

2017

Adv Funct Materials

157

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Atom/molecular-level controls in nanotechnology are important for the precise placement of components in device applications. Despite many advances, nanotechnology still uses simple systems to make precise atom/ molecule-scale changes. This is in contrast with the many phenomena observed in biological systems, where there appears to be a well-designed integrative approach involving molecular units to achieve atomic-and molecular-scale changes. Inspired by nature, we introduced a novel concept-nanoarchitectonics-to develop nanoscale functional materials for bio-oriented applications. Nanoarchitectonics is a unified concept combining nanotechnology and methodologies in related research fields, such as supramolecular chemistry, self-assembly, and self-organization, to satisfy the major features of nanosciences for the creation of functional materials or even devices and machines. This concept guides the harmonized assembly of nanoscale objects into higher order functional materials. In this Feature Article, recent research activities are introduced regarding the development of advanced functional materials of hybrid and related architectures on the basis of nanoarchitectonics from diverse contexts of organic and inorganic materials: i) from biology (DNA-based hybrid); ii) from geology (clay-based hybrid); iii) from 0D nanotechnology (fullerene-based hybrid); and iv) from other nanoarchitectonics. Therefore, this Feature Article showcases some examples of the nanoarchitectonic concepts at work in diverse material types.

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Decoupling the Direct and Indirect Biological Effects of ZnO Nanoparticles Using a Communicative Dual Cell‐Type Tissue Construct

Cited by 27 publications

References 45 publications

Role of Zn doping in oxidative stress mediated cytotoxicity of TiO2 nanoparticles in human breast cancer MCF-7 cells

Role of Zn doping in oxidative stress mediated cytotoxicity of TiO2 nanoparticles in human breast cancer MCF-7 cells

Bioprinting 3D microfibrous scaffolds for engineering endothelialized myocardium and heart-on-a-chip

Nanoarchitectonics for Hybrid and Related Materials for Bio‐Oriented Applications

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