Decontamination of Anodized Implant Surface With Different Modalities for Peri‐Implantitis Treatment: Lasers and Mechanical Debridement With Citric Acid

Htet, Moe; Madi, Marwa; Zakaria, Osama; Miyahara, Takashige; Wang, Xin; Lin, Zayar; Aoki, Kazuhiro; Kasugai, Shohei

doi:10.1902/jop.2016.150615

Cited by 36 publications

(52 citation statements)

References 44 publications

(69 reference statements)

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“…Some authors have reported that citric acid causes a reduction in biofilm in vitro but still leaves a biocompatible surface. 165,167,171 This is consistent with the present observations of 40% citric acid applied for 60 seconds giving good biofilm removal and no surface alterations.…”

Section: Discussionsupporting

confidence: 93%

“…The use of 40% citric acid has been recommended for debridement and decontamination of Ti implant surfaces, 165,166 even though it does not always achieve complete removal of biofilm. 167 The chemical interaction between 40% citric acid and the titanium surface after 1 minute results in changes such as pitting, an increase in the surface roughness, changes to the oxide layer and surface discolouration.…”

Section: Citric Acidmentioning

confidence: 99%

“…Past studies have suggested a combination approach will be more effective for biofilm removal than a single method based on citric acid alone. 165 This could be explored for citric acid, used after a physical debridement method has been applied.…”

Section: Other Approachesmentioning

confidence: 99%

“…221,222 From the work in this thesis and other published studies, it appears that Er:YAG lasers, glycine particles, citric acid and electrolysis are promising areas to explore further, since these treatments are likely to either enhance biocompatibility, or at the very least, will not lower the surface energy. 93,165,186,223 From the work described in this thesis (as summarized Table 7.3), a suggested combination approach would be as follows when a purely closed debridement approach was to be used:…”

Section: Combination Approachesmentioning

confidence: 99%

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Novel methods for debridement of dental implant surfaces contaminated by biofilm

Tran¹

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Section: Discussionsupporting

confidence: 93%

Section: Citric Acidmentioning

confidence: 99%

Section: Other Approachesmentioning

confidence: 99%

Section: Combination Approachesmentioning

confidence: 99%

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Novel methods for debridement of dental implant surfaces contaminated by biofilm

Tran¹

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“…The purpose of treatment in peri-implantitis is to decrease the bacterial load in the peri-implant pocket and decontaminate the implant surface to promote osseointegration [5]. Both surgical and non-surgical tools have been used to treat peri-implantitis, and there are various implant surface decontamination solutions, but consensus is still lacking regarding the most effective treatment in peri-implantitis [6]. However, the understanding of the pathogenesis of periimplantitis is incomplete and fragmented, and hence, the availability of an animal model to investigate inflammatory diseases might facilitate our understanding of the pathogenic mechanisms [7].…”

Section: Introductionmentioning

confidence: 99%

Inhibition of NF-κB by Pyrrolidine Dithiocarbamate Prevents the Inflammatory Response in a Ligature-Induced Peri-Implantitis Model: A Canine Study

Jiang²,

Wang

et al. 2018

Cell Physiol Biochem

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Background/Aims: The roles of toll-like receptor 4 (TLR4) and nuclear factor-kappa B (NF-κB) in peri-implantitis are unclear. Here, we used a canine model of peri-implantitis to explore the effects of inhibiting NF-κB with pyrrolidine dithiocarbamate (PDTC) on the inflammatory response in ligature-induced peri-implantitis. Methods: After successfully establishing the peri-implantitis model, beagles were randomly assigned to normal, model or PDTC groups. ELISA tests were used to determine the levels of interleukin (IL)-1, IL-6, IL-8 and tumor necrosis factor alpha (TNF-α). Immunohistochemistry was employed to assess the expression of NF-κB p65. Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) was performed to determine the mRNA levels of TLR4 and NF-κB p65, and western blot analysis was used to measure the protein levels of TLR4 in periodontal tissues from each group. Periodontal ligament fibroblasts (PDLFs) were cultured and subsequently classified into PDLF normal, PDLF model, PDLF LPS, PDLF PDTC, and PDLF LPS + PDTC groups. An immunofluorescence assay was used to measure the expression level of NF-κB p65. The CCK-8 assay and flow cytometry were performed to evaluate cell proliferation and apoptosis. Results: The in vitro results indicated that NF-κB p65 and TLR4 were upregulated in canine periodontal tissues, and PDTC could suppress the expression levels of NF-κB p65 and TLR4. Inflammation could increase TLR4 protein expression in canine periodontal tissue, and PDTC could inhibit the inflammation-induced increase in TLR4 protein expression. These results revealed that PDTC could reverse the LPS-induced increases in the levels of IL-1, IL-6, IL-8 and TNF-α. In vivo, the results demonstrated that PDTC inhibited the LPS-induced NF-κB p65 upregulation, and PDTC could reverse the inhibitory effect of the PDLF model + LPS on the proliferation of periodontal fibroblasts. The results also showed that in the PDLF model, LPS promoted PDLF apoptosis by inducing implant periodontitis in canines, but PDTC inhibited the PDLF apoptosis and relieved implant periodontitis in canines. Conclusion: Based on our results, we concluded that PDTC can inhibit the expression of NF-κB and alleviate the inflammatory response induced by LPS, thereby preventing periodontal inflammation and reducing the development of peri-implantitis.

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Efficiency of cold atmospheric plasma, cleaning powders and their combination for biofilm removal on two different titanium implant surfaces

et al. 2022

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Objectives Biofilm removal is the decisive factor for the control of peri-implantitis. Cold atmospheric pressure plasma (CAP) can become an effective aid due to its ability to destroy and to inactivate bacterial biofilm residues. This study evaluated the cleaning efficiency of CAP, and air-polishing with glycine (APG) or erythritol (APE) containing powders alone or in combination with CAP (APG + CAP, APE + CAP) on sandblasted/acid etched, and anodised titanium implant surface. Materials and methods On respective titanium discs, a 7-day ex vivo human biofilm was grown. Afterwards, the samples were treated with CAP, APG, APE, APG + CAP, and APE + CAP. Sterile and untreated biofilm discs were used for verification. Directly after treatment and after 5 days of incubation in medium at 37 °C, samples were prepared for examination by fluorescence microscopy. The relative biofilm fluorescence was measured for quantitative analyses. Results Air-polishing with or without CAP removed biofilms effectively. The combination of air-polishing with CAP showed the best cleaning results compared to single treatments, even on day 5. Immediately after treatment, APE + CAP showed insignificant higher cleansing efficiency than APG + CAP. Conclusions CAP supports mechanical cleansing and disinfection to remove and inactivate microbial biofilm on implant surfaces significantly. Here, the type of the powder was not important. The highest cleansing results were obtained on sandblasted/etched surfaces. Clinical relevance. Microbial residuals impede wound healing and re-osseointegration after peri-implantitis treatment. Air-polishing treatment removes biofilms very effectively, but not completely. In combination with CAP, microbial free surfaces can be achieved. The tested treatment regime offers an advantage during treatment of peri-implantitis.

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Decontamination of Anodized Implant Surface With Different Modalities for Peri‐Implantitis Treatment: Lasers and Mechanical Debridement With Citric Acid

Abstract: Within the limits of the study, the combination of mechanical and chemical treatment proved to be the most effective treatment for disinfection of the anodized implant surface.

Cited by 36 publications

References 44 publications

Novel methods for debridement of dental implant surfaces contaminated by biofilm

Novel methods for debridement of dental implant surfaces contaminated by biofilm

Inhibition of NF-κB by Pyrrolidine Dithiocarbamate Prevents the Inflammatory Response in a Ligature-Induced Peri-Implantitis Model: A Canine Study

Efficiency of cold atmospheric plasma, cleaning powders and their combination for biofilm removal on two different titanium implant surfaces

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