Decolourisation and detoxification of synthetic molasses melanoidins by individual and mixed cultures of Bacillus spp.

Kumar, Pramod; Chandra, Ram

doi:10.1016/j.biortech.2005.10.012

Cited by 109 publications

(85 citation statements)

References 16 publications

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“…Some of bacterial strains have been reported as a molasses decolorizing bacteria. They were Pseudomonas, Acenitobacter, Bacillus and Klebsiella present in this bacterial consortium, all of which could decolorize colored components present in molasses wastewater [22,29,40]. Many researchers also reported the activity of Serratia mercescens on biodegradation of polycyclic aromatic hydrocarbons (PAHs) and lignin degrading activity [43].…”

Section: Identification Of Bacterial Isolates In the Consortiummentioning

confidence: 82%

“…The mixed culture of Bacillus spp. exhibited a two-to fourfold increase in melanoidins decolorization over that showed by any individual Bacillus isolate [22]. Also, Alkane et al [1] reported that 69% decolorization of molasses spent wash was achieved using soil samples as inoculum instead of isolated microorganisms.…”

Section: Introductionmentioning

confidence: 98%

“…However, the performance of fungal decolorization was limited by long growth cycle and moderate decolorization rate. In contrast, the bacterial decolorization is usually faster, but it may require a mixed community to decolorize melanoidins through combined metabolic mode of individual culture [1,22]. The mixed culture of Bacillus spp.…”

Section: Introductionmentioning

confidence: 99%

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Decolorization of synthetic melanoidins-containing wastewater by a bacterial consortium

Jiranuntipon

Chareonpornwattana

Damronglerd

et al. 2008

J Ind Microbiol Biotechnol

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The presence of melanoidins in molasses wastewater leads to water pollution both due to its dark brown color and its COD contents. In this study, a bacterial consortium isolated from waterfall sediment was tested for its decolorization. The identification of culturable bacteria by 16S rDNA based approach showed that the consortium composed of Klebsiella oxytoca, Serratia mercescens, Citrobacter sp. and unknown bacterium. In the context of academic study, prevention on the difficulties of providing effluent as well as its variations in compositions, several synthetic media prepared with respect to color and COD contents based on analysis of molasses wastewater, i.e., Viandox sauce (13.5% v/v), caramel (30% w/v), beet molasses wastewater (41.5% v/v) and sugarcane molasses wastewater (20% v/v) were used for decolorization using consortium with color removal 9.5, 1.13, 8.02 and 17.5%, respectively, within 2 days. However, Viandox sauce was retained for further study. The effect of initial pH and Viandox concentration on decolorization and growth of bacterial consortium were further determined. The highest decolorization of 18.3% was achieved at pH 4 after 2 day of incubation. Experiments on fresh or used medium and used or fresh bacterial cells, led to conclusion that the limitation of decolorization was due to nutritional deficiency. The effect of aeration on decolorization was also carried out in 2 L laboratory-scale suspended cell bioreactor. The maximum decolorization was 19.3% with aeration at KLa = 2.5836 h -1 (0.1 vvm).

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Section: Identification Of Bacterial Isolates In the Consortiummentioning

confidence: 82%

Section: Introductionmentioning

confidence: 98%

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Decolorization of synthetic melanoidins-containing wastewater by a bacterial consortium

Jiranuntipon

Chareonpornwattana

Damronglerd

et al. 2008

J Ind Microbiol Biotechnol

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“…However, the long growth cycle and spore formation limit the performance of the fungal system. In contrast, bacterial decolourization is usually faster, but it may require a mixed culture to decolourize molasses wastewater through combined metabolic modes of bacterial strains 25,26 . Adikane and his co-workers 25 reported that 69% decolourization of molasses spent wash was achieved by using soil samples as inoculum instead of isolated microorganisms 25 .…”

Section: Introductionmentioning

confidence: 99%

“…Also, the mixed culture of Bacillus spp. exhibited a two-to four-fold increase in melanoidins decolourization over that shown by any individual Bacillus isolate 26 . Hence, mixed culture studies seem to be more promising for decolourization molasses wastewater.…”

Section: Introductionmentioning

confidence: 99%

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Jiranuntipon¹,

Délia²,

Albasi³

et al. 2009

ScienceAsia

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ABSTRACT:The effluent from sugarcane molasses based distilleries leads to environmental pollution due to its large volume and the presence of dark brown coloured compounds. In this study, a bacterial consortium isolated from waterfall sediments was selected as a molasses-decolourizing agent. The effect of culture conditions and medium composition on decolourization activity and growth of the bacterial consortium was investigated. The bacterial consortium was able to grow and decolourize molasses wastewater under facultative and anaerobic conditions in general. Aerobic culture conditions at pH 7 and 9 in molasses wastewater containing LB medium exhibited high growth but poor decolourization. The addition of a supplementary nutrient source in molasses wastewater medium significantly increased the decolourization activity of the bacterial consortium by up to 26.5% within 48 h under anaerobic conditions. Comparison of 16S rDNA sequences indicated that the bacterial consortium which showed decolourization activity under aerobic conditions consisted of Acinetobacter sp., Pseudomonas sp., Comamonas sp., Klebsiella oxytoca, Serratia marcescens and unidentified bacteria, whereas, the anaerobically enriched consortium consisted of Pseudomonas sp., Klebsiella oxytoca, Bacillus cereus and Citrobacter farmeri, a mercury-resistant bacterium, and an unidentified bacterium. Denaturing gradient gel electrophoresis profiles indicated that the community structures of aerobically and anaerobically enriched bacterial consortium were different.

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Commercial sugars as substrates for lipid accumulation in Cunninghamella echinulata and Mortierella isabellina fungi

Chatzifragkou

Fakas

Galiotou‐Panayotou

et al. 2010

Euro J Lipid Sci & Tech

108

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Cunninghamella echinulata and Mortierella isabellina, fungi capable of accumulating single cell oil (SCO) containing g-linolenic acid (GLA), were cultivated on sugar-based media, at initial substrate concentration 60 g/L. It was demonstrated that the carbon source plays an essential role on lipid accumulation process. Especially, on glucose, C. echinulata produced 3.9 g/L lipids containing 19.5% GLA, whereas M. isabellina achieved 9.9 g/L of lipid, containing 3.5% GLA. Growth on fructose was also satisfactory for both microorganisms. Although M. isabellina failed to grow adequately on saccharose, C. echinulata presented appreciable biomass production of 17.6 g/L, containing 18% of fat. Extra-cellular invertase activity was also detected in the case of C. echinulata cultures, achieving 0.5 U/mL. Both molds presented satisfactory growth on molasses, with significant amounts of SCO and GLA produced, accompanied by non-negligible substrate decolorization. Scalingup in a 3-L bioreactor of M. isabellina with glucose as a substrate resulted in 17.5 g/L of biomass, containing 12.7 g/L of lipid. Finally, fractionation of cellular lipid for both strains showed that neutral lipids represented about 90-92% w/w upon total accumulated lipids, whilst fractions of glycolipids plus sphingolipids and phospholipids were 6-7 and 3-4%, respectively.

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Decolourisation and detoxification of synthetic molasses melanoidins by individual and mixed cultures of Bacillus spp.

Cited by 109 publications

References 16 publications

Decolorization of synthetic melanoidins-containing wastewater by a bacterial consortium

Decolorization of synthetic melanoidins-containing wastewater by a bacterial consortium

Untitled

Commercial sugars as substrates for lipid accumulation in Cunninghamella echinulata and Mortierella isabellina fungi

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