Deciphering the molecular structure of cryptolepain in organic solvents

Pre-Molten, Wet, and Dry Molten Globules en Route to the Functional State of Proteins

“…MGs of globular proteins are generally obtained by their mild denaturation that can be induced by acid, alkali, low to medium concentrations of chemical denaturants such as urea and GdmHCl, chaotropic salts, moderately high temperature, and, for some proteins, even by low temperature [128][129][130][131][132][133][134][135][136][137][138][139][140][141][142][143][144][145][146][147]. Later studies revealed that in some proteins, an MG can also be induced by various organic solvents [148][149][150][151]. However, it was also shown that fluorinated alcohols can preferentially stabilize α-helices leading to the formation of non-native helical structures in some all-β-sheet proteins.…”

Pre-Molten, Wet, and Dry Molten Globules <i>En Route</i> to the Functional State of Proteins

“…MGs of globular proteins are generally obtained by their mild denaturation that can be induced by acid, alkali, low to medium concentrations of chemical denaturants, such as urea and GdmHCl, chaotropic salts, moderately high temperature, and, for some proteins, even by low temperature [128][129][130][131][132][133][134][135][136][137][138][139][140][141][142][143][144][145][146][147]. Later studies revealed that in some proteins, MG can be also induced by various organic solvents [148][149][150][151]. However, it was also shown that fluorinated alcohols can preferentially stabilize α-helices leading to the formation of non-native helical structure in some all-β-sheet proteins.…”