Decellularized human amniotic membrane: how viable is it as a delivery system for human adipose tissue‐derived stromal cells?

Gholipourmalekabadi, Mazaher; Zali, Hakimeh; Radenković, Dina; Mozafari, Masoud; Mossahebi-Mohammadi, Majid; Seifalian, Alexander M.

doi:10.1111/cpr.12240

Cited by 68 publications

(61 citation statements)

References 49 publications

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“…The MSCs were seeded on both HA/GEL and HA/GEL/OC nanocomposites and incubated for 3 days, as described in our last published work . Thereafter, the samples were rinsed with distilled water, fixed with gluteraldehyde 2.5% solution and dehydrated in a graded concentration (30, 50, 70, and 100%) of acetone (Merck, Germany).…”

Section: Methodsmentioning

confidence: 99%

Fabrication andin vivoevaluation of an osteoblast-conditioned nano-hydroxyapatite/gelatin composite scaffold for bone tissue regeneration

Samadikuchaksaraei

Gholipourmalekabadi

Ezadyar

et al. 2016

J. Biomed. Mater. Res.

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In this study, the effects of osteoblast-conditioning on mechanical behavior, biocompatibility, biodegradation and osteoinductive properties of a nano-hydroxyapatite/gelatin (HA/GEL) nanocomposite scaffold was investigated. The scaffold was fabricated using the layer solvent casting combined with the freeze-drying and lamination techniques. The scaffolds were conditioned by culture of osteoblasts on their surface and their elimination by a repeated freeze-thawing process. The potential of the osteoblast-conditioned HA/GEL (HA/GEL/OC) scaffold to support cell adhesion and growth and its cytotoxicity was assessed in vitro using rat mesenchymal stem cells. For in vivo studies, the HA/GEL/OC nanocomposite was implanted in the critical size bone defect created on rat calvarium and studied after 7, 30 and 90 days. The results showed that mechanical and in vitro biological properties of the scaffold were not affected by the process of conditioning. However, in vivo studies demonstrated that osteoblast-conditioning enhanced biocompatibility and osteoinductivity and of the nanocomposite scaffold. The osteoblast conditioning also accelerated collagen content during the bone healing. In the experimental group that received the HA/GEL/OC and MSCs, the newly formed bone occupied almost the entire defect (93.4 ± 3.3%) within 3 months. In conclusion, this study indicates that osteoblast-conditioning is a viable strategy for the development of bone tissue engineering scaffolds. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 104A: 2001-2010, 2016.

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Section: Methodsmentioning

confidence: 99%

Fabrication andin vivoevaluation of an osteoblast-conditioned nano-hydroxyapatite/gelatin composite scaffold for bone tissue regeneration

Samadikuchaksaraei

Gholipourmalekabadi

Ezadyar

et al. 2016

J. Biomed. Mater. Res.

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“…In a study by S anchez-S anchez and colleagues, AM were used as a biological scaffold for the culture and maintenance of hADMSCs, where the membranes were found to induce hADMSC to secrete IL-10 as well as IL-1b, whose interplay is thought to be vital for a balanced restoration of all necessary tissues [100]. Decellularized AM has also been found to be suitable as a scaffold for the delivery of hADMSC in tissue engineering and regenerative medicine applications [101]. Sheets of cultured dental pulp MSCs prepared for periodontal tissue repair, using AM as a scaffold, retained their stem cell phenotype while promoting cell proliferation [102].…”

Section: Fetal Membranes For Other Stem Cell Typesmentioning

confidence: 99%

Concise Review: Fetal Membranes in Regenerative Medicine: New Tricks from an Old Dog?

Lim

2017

Stem Cells Translational Medicine

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The clinical application of the fetal membranes dates back to nearly a century. Their use has ranged from superficial skin dressings to surgical wound closure. The applications of the fetal membranes are constantly evolving, and key to this is the uncovering of multiple populations of stem and stem‐like cells, each with unique properties that can be exploited for regenerative medicine. In addition to pro‐angiogenic and immunomodulatory properties of the stem and stem‐like cells arising from the fetal membranes, the dehydrated and/or decellularized forms of the fetal membranes have been used to support the growth and function of other cells and tissues, including adipose‐derived mesenchymal stem cells. This concise review explores the biological origin of the fetal membranes, a history of their use in medicine, and recent developments in the use of fetal membranes and their derived stem and stem‐like cells in regenerative medicine. Stem Cells Translational Medicine 2017;6:1767–1776

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“…3,6,[31][32][33][34][35][36][37][38][39][40][41] In addition, various cell types including fibroblasts and keratinocytes have been found to adhere to, proliferate, and function in the presence of amniotic scaffolds. [42][43][44][45] in vivo studies in man have substantiated these in vitro findings that these products improve the rate of wound healing. 24,25,46,47 Furthermore, several case series have documented the use of decellularized amniotic membrane scaffolds facilitating the healing of pharyngocutaneous fistulas, burns, bed sores, and chronic wounds that were nonresponsive to standard-of-care treatments in human patients.…”

Section: Introductionmentioning

confidence: 97%

“…Human amniotic membrane scaffold products have been reported to contain quantifiable levels of epidermal growth factor (EGF), vascular endothelial growth factor (VEGF), platelet derived growth factor (PDGF), transforming growth factor (TGF)‐β1, basic fibroblastic growth factor, and prostaglandin E 2 (PGE 2 ), each of which is known to a play role in wound healing . In addition, various cell types including fibroblasts and keratinocytes have been found to adhere to, proliferate, and function in the presence of amniotic scaffolds . in vivo studies in man have substantiated these in vitro findings that these products improve the rate of wound healing .…”

Section: Introductionmentioning

confidence: 99%

Effects of acellular equine amniotic allografts on the healing of experimentally induced full‐thickness distal limb wounds in horses

et al. 2019

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Objective To characterize the growth factors contained in equine amniotic membrane allograft (eAM; StemWrap scaffold and StemWrap+ injection) and to evaluate the effect of eAM on equine distal limb wound healing. Study design Prospective experimental controlled study. Sample population Eight adult horses. Methods Transforming growth factor (TGF)‐β1, vascular endothelial growth factor (VEGF), epidermal growth factor, platelet‐derived growth factor‐BB, and prostaglandin E2 (PGE2) concentrations in StemWrap+ were assessed with enzyme‐linked immunosorbent assay. Two full‐thickness 6.25‐cm2 skin wounds were created on each metacarpus. On one forelimb, one wound was treated with eAM, and the other was left untreated (eAM control). On the contralateral limb, one wound was treated with a silicone dressing, and the other served as negative control. Three‐dimensional images were obtained to determine wound circumference and surface area analyses at each bandage change until healed. Excessive granulation tissue was debrided once weekly for 4 weeks. Biopsy samples were taken to evaluate quality of wound healing via histologic and immunohistochemistry assays. Results StemWrap+ contained moderate concentrations of TGF‐β1 (494.10 pg/mL), VEGF (212.52 pg/mL), and PGE2 (1811.61 pg/mL). Treatment of wounds with eAM did not affect time to healing or histologic quality of the healing compared with other groups but was associated with increased granulation tissue production early in the study, particularly on day 7. Conclusion Application of eAM resulted in increased granulation tissue production while maintaining appropriate healing of experimental wounds. Clinical significance Use of eAM is likely most beneficial for substantial wounds in which expedient production of large amounts of granulation tissue is desirable.

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Decellularized human amniotic membrane: how viable is it as a delivery system for human adipose tissue‐derived stromal cells?

Abstract: These results strongly suggest that dHAMs have considerable potential as 3D cell-carrier scaffolds for delivery of hADSC, in tissue engineering and regenerative medicine applications.

Cited by 68 publications

References 49 publications

Fabrication andin vivoevaluation of an osteoblast-conditioned nano-hydroxyapatite/gelatin composite scaffold for bone tissue regeneration

Fabrication andin vivoevaluation of an osteoblast-conditioned nano-hydroxyapatite/gelatin composite scaffold for bone tissue regeneration

Concise Review: Fetal Membranes in Regenerative Medicine: New Tricks from an Old Dog?

Effects of acellular equine amniotic allografts on the healing of experimentally induced full‐thickness distal limb wounds in horses

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