volume 1, issue 3, P100184 2020
DOI: 10.1016/j.xpro.2020.100184
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Abstract: Summary Mosaic analysis by dual recombinase-mediated cassette exchange (MADR) is a technology that allows stable and locus-specific integration of transgenic elements into recipient cells carrying loxP and FRT sites. Nevertheless, most cell lines lack these recombination-specific sites. This protocol describes a method to introduce the minimum requirements into cells, leading to the generation of de novo primary MADR recipient cells or MADR “Proxy” cells. These cell lines allo…

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