DDX11-AS1exacerbates bladder cancer progression by enhancing CDK6 expression via suppressing miR-499b-5p

Li, Qiang; Wang, Sugui; Wu, Zhi‐Ying; Liu, Yuzhong

doi:10.1016/j.biopha.2020.110164

Cited by 15 publications

(15 citation statements)

References 27 publications

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“…23 DDX11-AS1 has also been reported to sponge several miRNAs, such as miR-499b-5p, miR-873, and miR-326. 13,14,19 In our study, we found that DDX11-AS1 was distributed in the cytoplasm of glioma cells. We also demonstrated that DDX11-AS1 was the sponge for miR-499b-5p by luciferase reporter assay and RNA pulldown assay.…”

Section: Discussionsupporting

confidence: 47%

“…DDX11-AS1 promotes the malignant behaviors of gastric cancer, bladder cancer, lung cancer, osteosarcoma, esophageal cancer, colorectal cancer, and liver cancer. [13][14][15]19,20 However, whether DDX11-AS1 regulates glioma is not investigated. In our work, we demonstrated that DDX11-AS1 expression was upregulated in glioma tissues.…”

Section: Discussionmentioning

confidence: 99%

“…Only one study showed that miR-499b-5p suppresses bladder cancer development. 14 Its function in other cancer is fully unknown. Our data showed that miR-499b-5p was downregulated in glioma tissues and its expression was restricted by DDX11-AS1.…”

Section: Discussionmentioning

confidence: 99%

“…However, only miR-499b-5p ranked top and has been reported to be sponged by DDX11-AS1 in bladder cancer. 14 Thus, we focused on miR-499b-5p and constructed wide-type (WT) and mutant (MUT) luciferase reporters (Figure 3B). Luciferase reporter assay suggested that miR-499b-5p mimics suppressed the activity of WT-DDX11-AS1 reporter (Figure 3C).…”

Section: Ddx11-as1 Knockdown Inhibited Glioma Progressionmentioning

confidence: 99%

“…DDX11-AS1 has been researched and identified as an oncogenic lncRNA in several cancers, such as gastric cancer, bladder cancer, and esophageal cancer. [13][14][15] Its role in glioma remains undefined. In the current study, DDX11-AS1 was found to be upregulated in glioma and correlated with poor prognosis.…”

Section: Introductionmentioning

confidence: 99%

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LncRNA DDX11-AS1 Exerts Oncogenic Roles in Glioma Through Regulating miR-499b-5p/RWDD4 Axis

Zheng¹,

Xie²,

Xu³

et al. 2021

OTT

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Background: Long noncoding RNAs (lncRNA) exert essential functions during tumorigenesis. However, how lncRNAs participate in glioma development remains poorly researched. This study aimed to determine how DDX11-AS1 affects glioma progression. Methods: Gene expression was analyzed by qRT-PCR. Survival rate curve was plotted in 56 glioma patients. Loss-of-function assays were performed to analyze proliferation, migration, and invasion through CCK8, colony formation, and transwell assays. Luciferase assay and RNA pulldown assays were conducted to illustrate the underlying molecular mechanism. Results: DDX11-AS1 expression was upregulated in glioma tissues and cells. DDX11-AS1 overexpression was linked with poor prognostic value. DDX11-AS1 knockdown suppressed proliferation, migration, and invasion while inducing apoptosis. DDX11-AS1 interacted with miR-499b-5p to eliminate it, leading to upregulation of RWDD4 expression. RWDD4 was upregulated in glioma while miR-499b-5p was downregulated. Conclusion: DDX11-AS1 upregulation promotes glioma progression through acting as a competing endogenous RNA for miR-499b-5p to upregulate RWDD4.

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Section: Discussionsupporting

confidence: 47%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Ddx11-as1 Knockdown Inhibited Glioma Progressionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

LncRNA DDX11-AS1 Exerts Oncogenic Roles in Glioma Through Regulating miR-499b-5p/RWDD4 Axis

Zheng¹,

Xie²,

Xu³

et al. 2021

OTT

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circRNA TATA‐box binding protein associated factor 15 acts as an oncogene to facilitate bladder cancer progression through targeting miR‐502‐5p/high mobility group box 3

Yang,

He,

Luo

et al. 2024

Molecular Carcinogenesis

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Circular RNAs (circRNAs) are key in regulating bladder cancer progression. This study explored the effects of circRNA TATA‐box binding protein associated factor 15 (circTAF15) on bladder cancer progression. We enrolled 80 bladder cancer patients to examine the relationship between circTAF15 expression and clinical features. The function of circTAF15 on bladder cancer cell viability, proliferation, migration, invasion, and glycolysis was monitored by cell counting kit‐8 assay, 5‐Ethynyl‐2′‐deoxyuridine experiment, Transwell experiment, and glycolysis analysis. Dual luciferase reporter gene assay, RNA pull‐down assay, and RNA immunoprecipitation assay were used to verify the binding between circTAF15 and miR‐502‐5p or between miR‐502‐5p and high mobility group box 3 (HMGB3). circTAF15 effect on in vivo growth of bladder cancer was investigated by xenograft tumor experiment. Quantitative real‐time polymerase chain reaction, Western blot, and immunohistochemistry were implemented to investigate the expression levels of genes. circTAF15 was upregulated in bladder cancer patients, associated with unfavorable outcomes. circTAF15 knockdown attenuated bladder cancer cell viability, proliferation, migration, invasion, epithelial‐mesenchymal transition, and glycolysis. circTAF15 suppressed miR‐502‐5p expression, and miR‐502‐5p inhibited HMGB3 expression. Low miR‐502‐5p expression was associated with unfavorable outcomes in bladder cancer patients. miR‐502‐5p silencing and HMGB3 overexpression counteracted the inhibition of circTAF15 knockdown on the malignant phenotype of bladder cancer cells. circTAF15 knockdown attenuated the in vivo growth of bladder cancer cells. circTAF15 enhanced the progression of bladder cancer through upregulating HMGB3 via suppressing miR‐502‐5p. circTAF15 may be a novel target to treat bladder cancer in the future.

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Knockdown of Circ_0000798 Impedes Cell Growth and Motility of Renal Cell Carcinoma Cells Through Functioning as miRNA Sponge for miR-589-5p

Zheng

et al. 2022

Biochem Genet

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DDX11-AS1exacerbates bladder cancer progression by enhancing CDK6 expression via suppressing miR-499b-5p

Cited by 15 publications

References 27 publications

LncRNA DDX11-AS1 Exerts Oncogenic Roles in Glioma Through Regulating miR-499b-5p/RWDD4 Axis

LncRNA DDX11-AS1 Exerts Oncogenic Roles in Glioma Through Regulating miR-499b-5p/RWDD4 Axis

circRNA TATA‐box binding protein associated factor 15 acts as an oncogene to facilitate bladder cancer progression through targeting miR‐502‐5p/high mobility group box 3

Knockdown of Circ_0000798 Impedes Cell Growth and Motility of Renal Cell Carcinoma Cells Through Functioning as miRNA Sponge for miR-589-5p

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