DCBLD1 Overexpression Is Associated With a Poor Prognosis in Head and Neck Squamous Cell Carcinoma

Abstract

BackgroundDCBLD1 is highly expressed in several kinds of cancer and plays a potential prognostic factor. However, the prognostic value and immune infiltration in head and neck squamous cell carcinoma remain unclear and need further research.Materials and MethodsDCBLD1 expression and clinical information were obtained from the Cancer Genome Atlas (TCGA) database. The mRNA level in cell lines (SCC25 and CAL27) and gingival fibroblasts were detected using quantitative PCR. Cox regression analysis was used to evaluate the prognostic values of DCBLD1 and clinical data in HNSCC. A nomogram was also established to predict the impact of DCBLD1 on prognosis based on Cox multivariate results. The methylation level of DCBLD1 in HNSC and its prognosis were analyzed in UALACN and MethSurv. Finally, the potential biological functions of DCBLD1 were investigated using gene set enrichment analysis (GSEA) and single-sample GSEA (ssGSEA).ResultsThe mRNA and protein expression levels of DCBLD1 were highly expressed in HNSCC tissue and cell lines. The Cox analyses demonstrate that highly expressed DCBLD1 is an independent prognosis marker (p < 0.05). ROC curve analysis showed the performance of DCBLD1 (area under the ROC curve: 0.948, sensitivity: 93.2%, specificity: 84.7%). The methylation was increased in HNSCC patients compared with normal subjects (p < 0.05) and was associated with poor prognosis at sites cg27642470 and cg21104965. Additionally, DCBLD1 expression is poorly associated with immune cell infiltration and immunological checkpoints PD-L1 and TIM-3.ConclusionIn head and neck squamous cell carcinoma, DCBLD1 is overexpressed, associated with poor patient prognosis. The detailed underlying mechanism merits further research.

T cell proliferation-related subtypes, prognosis model and characterization of tumor microenvironment in head and neck squamous cell carcinoma

“…Risk score, clinical stage, and age are found to be important prognostic factors. As nomograms have been widely used as a predictive tool for cancer [ 33 , 34 ], to better understand the clinical characteristics and role of risk scores, we developed nomogram models and validated their high predictive efficiency. As mentioned above, our risk score strongly influenced the prognosis of patients with HNSC.…”

Identify an Innovative Fatty Acid Metabolism-Related Gene in Head and Neck Squamous Cell Carcinoma

“…Recently, studies have suggested that HNSCC is rich with in ltrated immune cells, the progression and prognosis of cancer are tightly related to immune cell in ltration 22 . Furthermore, fatty acid metabolism is directed at supporting speci c cell functions, since the nal outcome of an immune response is determined by cell metabolism 23 .…”

T cell proliferation-related subtypes, prognosis model and characterization of tumor microenvironment in head and neck squamous cell carcinoma

“…Risk score, clinical stage, and age are found to be important prognostic factors. As nomograms have been widely used as a predictive tool for cancer [33,34], to better understand the clinical characteristics and role of risk scores, we developed nomogram models and validated their high predictive e ciency. As mentioned above, our risk score strongly in uenced the prognosis of patients with HNSC.…”