Daucosterol linolenate from Sweet Potato Suppresses MCF7-Xenograft-Tumor Growth through Regulating PI3K/AKT Pathway

Abstract

Sweet potato is a functional food with potential antitumor properties, but the bioactive constituents and biological mechanisms remain unclear. In this study, we investigated the antitumor effect of daucosterol linolenate extracted from sweet potato and its potential mechanism. An MTT assay indicated that DLA inhibited the proliferation of breast cancer MCF-7 cells but had only weak effects on the proliferation of MDA-MB-231, 4T1, and MCF-10A cells. Flow cytometry analysis revealed that daucosterol linolenate induced apoptosis of MCF-7 cells. Experiments with MCF-7 xenograft in nude mice further confirmed that DLA inhibited tumor growth dose-dependently. After DLA treatment, the expressions of B-cell lymphoma 2 and vascular endothelial growth factor were decreased and that of cleaved caspase 3 was increased as compared to the TC group. DLA also down-regulated the expression of phosphoinositide 3-kinase/protein kinase B and repressed insulin-induced phosphoinositide 3-kinase/protein kinase B activation. Our findings suggest that DLA suppresses breast tumor growth through inactivating the phosphoinositide 3-kinase/protein kinase B pathway.

Immune-Enhancing Effects of a Novel Glucan from Purple Sweet Potato Ipomoea batatas (L.) Lam on RAW264.7 Macrophage Cells via TLR2- and TLR4-Mediated Pathways

“…Sweet potato (Ipomoea batatas) is a kind of both edible and medicinal agricultural food, which provides a dietary source of vitamins, minerals, carbohydrates, proteins, anthocyanins, essential fatty acids, trace elements, and other nutrients, and these active substances play roles in many pharmacological activities such as antitumor, immune regulation, hepatoprotective effect, hypoglycemic effect, hypolipidemic effect, antiaging, intestinal regulation, , antiobesity, antifatigue, and so on . Some water-soluble bioactive polysaccharides and alkali-soluble bioactive polysaccharides have been purified from purple sweet potato. − In our recent report, a new 18.3 kDa homogeneous polysaccharide component named PSPP-1 was isolated from purple sweet potato, and its structures were elucidated comprehensively for the first time, which was very useful for its further structure–activity relationship study.…”

Exploring the Biological Mechanism of Huang Yam in Treating Tumors and Preventing Antitumor Drug-Induced Cardiotoxicity Using Network Pharmacology and Molecular Docking Technology

“…Zhang et al [ 44 ] found that daucosterol can protect cells and reduce apoptosis by inhibiting the PI3K-Akt-mTOR signaling pathway. Han [ 32 , 45 ] found that daucosterol can inhibit tumor growth by affecting the PI3K-Akt-mTOR signaling pathway.…”