Data supporting chitosan facilitates structure formation of the salivary gland by regulating the basement membrane components

Abstract

To investigate the role of basement membrane (BM) in chitosan-mediated morphogenesis of the salivary glands, the embryonic submandibular gland (SMG) experimental model was used. Chitosan promotes branching at distinct stages in SMG morphogenesis. When enzymes such as type IV collagenase, dispase, and cathepsin B were used to digest the BM components, the morphogenetic effect mediated by chitosan disappeared. Immunofluorescence revealed that the corresponding receptors for BM components, including CD49c, CD49f, CD29, and dystroglycan, were locally enriched at the epithelial–mesenchymal junction around BM areas. The functional roles of laminin α1 and α5 in SMG branching were explored via siRNA knockdown, and suppression was confirmed at both the RNA and protein levels (Yang and Hsiao, Biomaterials, http://dx.doi.org/10.1016/j.biomaterials.2015.06.028, 2015). This data article demonstrates the experimental approaches to investigate the role of basement membrane in the structure formation of the salivary gland engineered by biomaterials.

Chitosan facilitates structure formation of the salivary gland by regulating the basement membrane components

“…2b in Ref. [20]). Furthermore, following treatment with cathepsin B, which is capable of digesting laminin (LN) [31], branching still occurred in both groups but with reduced branch numbers (Fig.…”

“…2a in Ref. [20]) [29]. Dispase possesses both fibronectinase and type IV collagenase activities [30].…”

“…The details were described in the supporting materials in Ref. [20]. Animal protocols were approved by the Animal Care and Use Committee of the National Taiwan University and were in accordance with the guidelines.…”

“…1aed in Ref. [20]) [19,26,27]. The cultured explants were first treated with enzymes to digest specific BM components.…”

“…Preparation of the chitosan-containing system followed the established protocol [15], and the details were described in Ref. [20].…”