Data for comparative proteomics analysis of the antitumor effect of CIGB-552 peptide in HT-29 colon adenocarcinoma cells

Abstract

CIGB-552 is a second generation antitumor peptide that displays potent cytotoxicity in lung and colon cancer cells. The nuclear subproteome of HT-29 colon adenocarcinoma cells treated with CIGB-552 peptide was identified and analyzed [1]. This data article provides supporting evidence for the above analysis.

Comparative proteomics analysis of the antitumor effect of CIGB-552 peptide in HT-29 colon adenocarcinoma cells

“…Similar patterns of protein bands were obtained after the SDS-PAGE analysis of nuclear fractions of both, control and CIGB-552 treated cells (See Figure 3 in [13]). The study was performed using the DF-PAGE method [10,11,15] (See Figure 2 in [13]). DF-PAGE combines protein fractionation by SDS-PAGE, ingel protein digestion and peptide fractionation by SDS-free PAGE.…”

“…This showed that 466 of the identified proteins, including 49 differentially modulated, can localize to nucleus (See Table 2 in [13]). The detection of not nuclear localized proteins suggests that contamination occurred during fraction enrichment.…”

“…UniProtKB (See Figure 4 in [13]). This showed that 466 of the identified proteins, including 49 differentially modulated, can localize to nucleus (See Table 2 in [13]).…”

“…As the samples from control and treated cells were up-regulated) ( Table 1, See Table 1 in [13]). These proteins were functionally analyzed using a bioinformatics workflow that involved subcellular location classification, singular and modular enrichment analysis and information retrieval and text mining [12] (See Figure 4,5 in [13]). …”

“…A total of 723 proteins were identified, 622 of them (85%) were identified in only one SDS-PAGE fraction (See Table 1 in [13]). As the samples from control and treated cells were up-regulated) ( Table 1, See Table 1 in [13]).…”