Damage to the cytoplasmic membrane of Escherichia Coli by catechin-copper (II) complexes

Hoshino, Nobuo; Kimura, Takeshi; Yamaji, Akihiro; Ando, Takashi

doi:10.1016/s0891-5849(99)00157-4

Cited by 87 publications

(70 citation statements)

References 17 publications

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“…Electron microscopic observation revealed that the cytoplasm membrane was only damaged but that nucleus and organelle remained intact, demonstrating that the first target of EGCG and its derivatives to LNCaP cells was the cytoplasm membrane. This is in agreement with that of Hoshino et al(1999) who found that the recycling redox reactions occurring between Cu 2+ and Cu + in the microbe system involving catechins and hydrogen peroxide, caused the cytoplasm membrane damages. Some previous studies showed that EGCG could induce apoptosis of cancer cells, including prostate cancer cell (Roy et al, 2003;Paschka et al, 1998;Gupta et al, 2000;Liang et al, 1997;Lin, 2002).…”

Section: Configuration Of Lncap Cells Detected By Electron Microscopesupporting

confidence: 93%

Cytotoxicity of epigallocatechin-3-gallate to LNCaP cells in the presence of Cu2+

Shen

Xiong³

2005

J Zheijang Univ Sci B

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Epigallocatechin-3-gallate (EGCG) has shown remarkably anti-cancer activity, with its bioactivity being related to reactive conditions, such as pH and metal ions. The present study investigated the degradation of EGCG and its effect on prostate cancer cell in the presence of Cu 2+. EGCG was incubated with prostate cancer cells, LNCaP, pretreated with or without Cu 2+ . EGCG in F-12 medium was quantified using HPLC and the viability of cells was assessed by gel electrophoresis, flow cytometry, and electron microscope. The results of HPLC showed that EGCG degraded completely within 12 h in F-12 medium with or without Cu 2+. Gel electrophoresis and flow cytometry did not detect apoptosis of LNCaP cells when they were incubated with EGCG. Electron microscopy examination revealed that EGCG-Cu 2+ complex led to damage of cytoplasm membrane in LNCaP cells. It was speculated that not EGCG, but its oxide and complex with Cu 2+ , are the bioactive components responsible for its cytotoxicity to LNCaP prostate cancer cells.

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Section: Configuration Of Lncap Cells Detected By Electron Microscopesupporting

confidence: 93%

Cytotoxicity of epigallocatechin-3-gallate to LNCaP cells in the presence of Cu2+

Shen

Xiong³

2005

J Zheijang Univ Sci B

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“…Miura et al (1998) detected H 2 O 2 in EC and (+)-catechin solutions incubated at 21˚C. Hoshino et al (1999) reported that H 2 O 2 was formed in a buffer containing EGC in the presence of Cu (II). Long et al (2000) found that H 2 O 2 was time-dependently generated in various cell-culture media after incubation of (+)-catechin, EGC, EGCg and gallic acid.…”

Section: Discussionmentioning

confidence: 99%

“…EGC possesses a benzene ring with three hydroxyl groups referred to as a gallyl moiety, which has been ascribed to its antioxidant activity. In addition, EGC, EGCg and compounds with a gallyl moiety have been reported to produce hydrogen peroxide (H 2 O 2 ) during oxidation in aqueous media (Hoshino et al, 1999;Long et al, 2000;Yang et al, 2000). This suggests that H 2 O 2 generation might be responsible for the biological activities.…”

mentioning

confidence: 99%

Mechanisms and Structural Specificity of Hydrogen Peroxide Formation during Oxidation of Catechins.

Nakayama¹,

Ichiba²,

Kuwabara³

et al. 2002

FSTR

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“…In P. syringae, being a gram-negative bacterium, a similar mechanism of action might be inhibiting the bacterial growth. Disruption of plasma membrane is one of the mechanisms of action of copper in microorganisms (Englander & Corden, 1971;Hoshino et al, 1999). It seems that binding of copper ions to the surface of bacterial cells plays an important role in the bactericidal activity (Hoshino et al, 2000).…”

Section: P Syringaementioning

confidence: 99%

In vitro effects of copper nanoparticles on plant pathogens, beneficial microbes and crop plants

Banik

Pérez‐de‐Luque

2017

Span. j. agric. res.

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Copper-based chemicals are effectively used as antimicrobials in agriculture. However, with respect to its nanoparticulate form there has been limited number of studies. In this investigation, in vitro tests on effect of copper nanoparticles (CuNPs) against plant pathogenic fungi, oomycete, bacteria, beneficial microbes Trichoderma harzianum and Rhizobium spp., and wheat seeds were conducted. Integration of CuNPs with non-nano copper like copper oxychloride (CoC) at 50 mg/L concentration each recorded 76% growth inhibition of the oomycete Phytophthora cinnamomi in vitro compared to the control. CuNPs also showed synergistic inhibitory effect with CoC on mycelial growth and sporulation of A. alternata. Pseudomonas syringae was inhibited at 200 mg/L of CuNPs. CuNPs were not significantly biocidal against Rhizobium spp. and Trichoderma harzianum compared to CoC. Evaluation of the effect of CuNP on wheat revealed that rate of germination of wheat seeds was higher in presence of CuNPs and CoC compared to control. Germination vigor index, root length, shoot dry weight and seed metabolic efficiency of wheat were negatively affected. At low concentration, CuNPs promoted the growth of the plant pathogenic fungi Botrytis fabae, Fusarium oxysporum f.sp. ciceris, F.oxysporum f.sp. melonis, Alternaria alternate and P. syringae, and sporulation of T. harzianum. Synergistic effect of CuNPs and CoC in inhibiting P. cinnamomi offers a possibility of developing new fungicide formulation for better control of the oomycetes. Non-biocidal effect of CuNPs against beneficial microbes indicates its potential use in the agri-ecosystem.

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Damage to the cytoplasmic membrane of Escherichia Coli by catechin-copper (II) complexes

Cited by 87 publications

References 17 publications

Cytotoxicity of epigallocatechin-3-gallate to LNCaP cells in the presence of Cu2+

Cytotoxicity of epigallocatechin-3-gallate to LNCaP cells in the presence of Cu2+

Mechanisms and Structural Specificity of Hydrogen Peroxide Formation during Oxidation of Catechins.

In vitro effects of copper nanoparticles on plant pathogens, beneficial microbes and crop plants

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