Damage and regeneration of hair cell ciliary bundles in a fish ear following treatment with gentamicin

Lombarte, Antoni; Yan, Hong; Popper, Arthur N.; Chang, Janet S.; Platt, Christopher

doi:10.1016/0378-5955(93)90002-i

Cited by 122 publications

(104 citation statements)

References 27 publications

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“…In contrast to mammals, many fish species have the ability to regenerate their inner ear and lateral line hair cells after damage (Kaus, 1987;Lombarte et al, 1993;Song et al, 1995;Scholik and Yan, 2001;Faucher et al, 2006;Smith et al, 2006). Given their capacity for regeneration, fish inner ear hair cells are nowadays a suitable model for investigations on hair cell regeneration and hearing function in animals.…”

Section: Introductionmentioning

confidence: 99%

“…Given their capacity for regeneration, fish inner ear hair cells are nowadays a suitable model for investigations on hair cell regeneration and hearing function in animals. In this scope, it has been common to use aminoglycoside antibiotics to damage fish inner ear hair cells and then observe the regenerative process (Matsuura et al, 1971;Lombarte et al, 1993). These antibiotics are known to displace calcium ions from their receptors, thereby blocking the cation channels that are located at the apices of hair cell stereocilia (Hudspeth, 1983;Kroese et al, 1989).…”

Section: Introductionmentioning

confidence: 99%

“…In contrast, intramuscular injections of gentamicin have led to damage of inner ear hair cells in freshwater fish (Yan et al, 1991;Lombarte et al, 1993). This aminoglycoside antibiotic, however, selectively damaged sensory hair cells in the inner ear lagena and utriculus of the teleost species, oscar (Astronotus ocellatus).…”

Section: Introductionmentioning

confidence: 99%

“…Following intrasaccular aminoglycoside injections, no adverse health effects were reported. After intramuscular injections of gentamicin, health disorders were common in the fish: loss of appetite, erratic swimming behaviour associated with weight gain, bloating of the abdomen and swelling around the eyes, indicative of osmoregulatory problems (Yan et al, 1991;Lombarte et al, 1993). Gentamicin causes renal injury after peritoneal injection in the goldfish, C. auratus (Liu et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

“…After exposure to aminoglycosides, inner ear hair cells regenerate within a period of between few days and about 20 days post-intervention in the oscar fish, A. ocellatus (Lombarte et al, 1993).…”

Section: Introductionmentioning

confidence: 99%

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Effects of systemic versus local gentamicin on the inner ear in the Atlantic cod, Gadus morhua (L.), relevance for fish hearing investigations

et al. 2008

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a b s t r a c tFish models are increasingly being used for hearing research investigations. Aminoglycoside antibiotics that are used for damaging the inner ear hair cells can have systemic side effects leading to death of study animals. This study aimed to compare two methods: (i) systemic (intravenous) and (ii) local (intrasaccular) gentamicin administration for induction of inner ear hair cell damage in the Atlantic cod, Gadus morhua (L.). Hair cell damage was assessed using scanning electron microscopy; hair cell density, prevalence of immature hair cells and kinocilia length were measured. Gentamicin-treated fish were compared with control and sham fish. Intravenous gentamicin led to dose-dependent mortality caused by nephrotoxicity. The only visible effect after treatment was more immature hair cells and shorter kinocilia, the effect on hair cell density was equivocal. Following intrasaccular gentamicin treatment, fish mortality was negligible, and hair cells were damaged regardless of dose. Here, we observed decreased hair cell density, high prevalence of immature hair cells, and significantly shortened kinocilia. Conclusion: intrasaccular injection is preferable to intravenous injection of gentamicin for the study of ototoxicity in the Atlantic cod.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Effects of systemic versus local gentamicin on the inner ear in the Atlantic cod, Gadus morhua (L.), relevance for fish hearing investigations

et al. 2008

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Induction of cell proliferation in avian inner ear sensory epithelia by insulin-like growth factor-I and insulin

1997

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Postembryonic production of inner-ear hair cells occurs both normally and after insult in lower vertebrates and avians. To determine how this proliferation is controlled, several growth factors were tested for effects on progenitor-cell division in cultured avian vestibular sensory epithelium. Mitogenic effects of bombesin, epidermal growth factor, insulin-like growth factor-I (IGF-I), insulin, and transforming growth factor-a were assayed in organotypic cultures of utricles from the mature, undamaged (normal) chicken inner ear. Tritiated thymidine and autoradiographic techniques and 5-bromo-28-deoxyuridine (BrdU) immunocytochemistry were used to identify cells synthesizing DNA. IGF-I stimulated DNA synthesis in the vestibular sensory receptor epithelium in a dose-dependent manner. DNA synthesis was also stimulated by insulin. These results suggest that stimulation of the IGF-I receptors by IGF-I or insulin binding stimulates cell proliferation in the mature avian vestibular sensory epithelium.

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Macrophage and microglia-like cells in the avian inner ear

1998

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Recent studies suggest that macrophages may influence early stages of the process of hair cell regeneration in lateral line neuromasts; numbers of macrophages were observed to increase prior to increases in hair cell progenitor proliferation, and macrophages have the potential to secrete mitogenic growth factors. We examined whether increases in the number of leukocytes present in the in vivo avian inner ear precede the proliferation of hair cell precursors following aminoglycoside insult. Bromodeoxyuridine (BrdU) immunohistochemistry was used to identify proliferating cells in chicken auditory and vestibular sensory receptor epithelia. LT40, an antibody to the avian homologue of common leukocyte antigen CD45, was used to label leukocytes within the receptor epithelia. Macrophages and, surprisingly, microglia-like cells are present in normal auditory and vestibular sensory epithelia. After hair cell loss caused by treatment with aminoglycosides, numbers of macrophage and microglialike cells increase in the sensory epithelium. The increase in macrophage and microglia-like cell numbers precedes a significant increase in sensory epithelial cell proliferation. The results suggest that macrophage and microglia-like cells may play a role in releasing early signals for cell cycle progression in damaged inner ear sensory epithelium.

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Damage and regeneration of hair cell ciliary bundles in a fish ear following treatment with gentamicin

Cited by 122 publications

References 27 publications

Effects of systemic versus local gentamicin on the inner ear in the Atlantic cod, Gadus morhua (L.), relevance for fish hearing investigations

Effects of systemic versus local gentamicin on the inner ear in the Atlantic cod, Gadus morhua (L.), relevance for fish hearing investigations

Induction of cell proliferation in avian inner ear sensory epithelia by insulin-like growth factor-I and insulin

Macrophage and microglia-like cells in the avian inner ear

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