Dalmatian Sage. Part 1. Differing Oil Yields and Compositions from Flowering and Non‐Flowering Accessions

Abstract

The components in essential oils of Dalmatian sage, Salvia officinalis L., were identified by 'H-and "C-NMR spectroscopy and by GC-MS. The diterpene manool was identified for the first time in steam-distilled oil from Dalmatian sage. Several sesquiterpenes were also identified for the first time in Dalmatian sage oils. A rapid GC method was developed and used to analyse 13 oils from a preliminary study of Dalmatian sage accessions. Oil yields from pilot-scale distillations in summer were significantly higher from non-flowering accessions than from flowering accessions. Oils from flowering and non-flowering accessions had different compositions, with significantly higher levels of thujones, B-caryophyllene and viridiflorol in oils from flowering accessions.

Essential Oils from Dalmatian Sage (Salvia officinalis L.): Variations among Individuals, Plant Parts, Seasons, and Sites

“…These oils had total thujone levels significantly (P < 0.05) lower than those of any of the flowering accessions (Table 1). There were also significant differences between nonflowering and flowering accessions for many other components, confirming the compositional differences that we noted previously (Perry et al, 1996). It may be that the nonflowering types of Dalmatian sage (MAFF, 1980) have been propagated from sterile hybrids of S. officinalis with another Salvia species that had distinct mono-and sesquiterpene chemistry.…”

“…The results of GC analyses (Perry et al, 1996) of the 67 oils were subjected to principal component analysis (PCA) to try to distinguish compositional patterns (Aries et al, 1991). The first and second principal components (PCs) accounted for 83% of the total variance of the data set.…”

“…Injections (0.5 µL) were made into a split (100:1) injector at 260 °C, the column temperature was programmed from 50 to 250 °C at 30 °C/min, and the flame ionization detector was at 350 °C. The levels (peak area as percent of total of peak areas) of 27 peaks, previously identified by GC/MS (Perry et al, 1996), were used for statistical analyses with SAS Institute software (see Supporting Information for full GC results). The unscaled levels of these 27 peaks in each oil were initially analyzed using principal components analysis to help with recognition of composition patterns.…”

Organ- and Season-Dependent Variation in the Essential Oil Composition of Salvia officinalis L. Cultivated at Two Different Sites

“…The isomers cis - and trans -2-methyl-3-methylenehept-5-ene have been named by some authors as cis - and trans -salvene (). The occurrence of the diterpene manool in sage essential oils had already been reported ( , ). All three of these compounds are absent in the 33 compound list of the essential oils from an original Portuguese S. officinalis clone (Coimbra rose, ref B3S2) reported by Chalchat et al ().…”

Gas Chromatography-Mass Spectrometry (GC-MS) Analysis of Essential Oil <em>Salvia Officinalis</em> in Sudan

“…The chemical composition of the essential oil of sage of different regions in the Mediterranean basin such as the former Yugoslavia [8,9] Italy [10,11] Egypt [12] Morocco [13] and Tunisia [14]etc.…”