Daidzein-rich isoflavone aglycones inhibit cell growth and inflammation in endometriosis

Takahashi, Osamu; Mori, Taisuke; Ito, Fumihiko; Okimura, Hiroyuki; Kataoka, Hideki; Tanaka, Yukiko; Koshiba, Akemi; Izumi, Kouji; Shigehiro, Sekiko; Amami, T.; Kitawaki, Jo

doi:10.1016/j.jsbmb.2018.04.004

Cited by 50 publications

(35 citation statements)

References 40 publications

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“…Other investigators showed that genistein caused regression of an endometriotic implant in a rat model . Our group recently demonstrated that daidzein‐rich isoflavone aglycones (DRIAs) inhibited the proliferation of OESCs at clinically feasible concentrations (Figure ). Additionally, DRIAs suppressed the formation of endometriosis‐like lesions in a mouse model .…”

Section: Estrogen Receptors In Endometriosismentioning

confidence: 93%

Local estrogen formation and its regulation in endometriosis

Mori

Ito

Koshiba

et al. 2019

Reprod Medicine & Biology

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Background It has been well established that endometriosis is an estrogen‐dependent disease. Although the exact pathogenesis of the disease is still unclear, it is known to be characterized by estrogen‐dependent growth and maintenance of the ectopic endometrium and increased local estrogen production. Methods The authors reviewed studies on local estrogen production and estrogen activities mediated by estrogen receptors in endometriotic tissues. Main findings Aberrant expression of several enzymes in local endometriotic lesions contributed to the production and metabolism of estrogens. Aromatase was one of the key therapeutic targets for the regulation of local estrogen formation. Our findings suggest that PGC‐1a, a transcriptional coactivator‐modulating steroid hormone, regulates aromatase expression and activity. Estrogen activities mediated by different types of estrogen receptors abnormally elevated in local tissues could also be involved in the development of endometriosis. The authors demonstrated that the isoflavone aglycone, a partial agonist of the estrogen receptor, suppressed the formation of endometriotic lesions. Conclusions Local estrogen production and estrogen activity mediated by estrogen receptors are important potential therapeutic targets for endometriosis.

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Section: Estrogen Receptors In Endometriosismentioning

confidence: 93%

Local estrogen formation and its regulation in endometriosis

Mori

Ito

Koshiba

et al. 2019

Reprod Medicine & Biology

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“…Other investigators showed that genistein caused regression of an endometriotic implant using a rat model . Our group recently demonstrated that daidzein‐rich isoflavone aglycones (DRIA) suppressed cell proliferation in oral epithelial but not in neuroepithelial stem cells at clinically feasible concentrations . The effect was most likely mediated by PGE 2 formation via the inhibition of aromatase expression/activity and COX‐2 expression.…”

Section: Aromatase‐targeting Treatment In Endometriosismentioning

confidence: 90%

“…The effect was most likely mediated by PGE 2 formation via the inhibition of aromatase expression/activity and COX‐2 expression. In addition, DRIA inhibited the formation of endometriosis‐like lesions in an in vivo mouse model, indicating that they might be useful for the treatment of endometriosis (Fig. ).…”

Section: Aromatase‐targeting Treatment In Endometriosismentioning

confidence: 92%

“…63 Our group recently demonstrated that daidzein-rich isoflavone aglycones (DRIA) suppressed cell proliferation in oral epithelial but not in neuroepithelial stem cells at clinically feasible concentrations. 64 The effect was most likely mediated by PGE 2 formation via the inhibition of aromatase expression/activity and COX-2 expression. In addition, DRIA inhibited the formation of endometriosis-like lesions in an in vivo mouse model, indicating that they might be useful for the treatment of endometriosis 64 (Fig.…”

Section: Aromatase-targeting Treatment In Endometriosismentioning

confidence: 99%

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Aromatase as a target for treating endometriosis

Mori

Ito

Koshiba

et al. 2018

J of Obstet and Gynaecol

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Endometriosis is a common gynecological disease that causes various clinical symptoms, such as chronic pelvic pain, dysmenorrhea and infertility, seriously affecting women's health and their quality of life. The symptoms and endometriotic lesions are relieved, in many cases, after menopause, when estrogen levels are lowered. Therefore, endometriosis is considered to be estrogen-dependent. Aromatase, the enzyme responsible for the last step of estrogen biosynthesis converting testosterone and androgen to estrogen, was previously reported to be more abundant in endometriotic tissues than in the normal endometrium, leading to an increased local estrogen concentration. Therefore, aromatase is considered a key therapeutic target for regulating local estrogen biosynthesis in endometriosis. A more complete understanding of the mechanisms that modulate aromatase and its activity is required to develop novel estrogen-targeted therapies for endometriosis. In this review article, we outline the current understanding of the pathological processes involved in estrogen production in endometriosis and propose novel strategies to treat this disorder.

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“…Isoflavones have estrogenic effects, genistein is able to prevent osteoporosis [2]. Daidzein and genistein at doses of 0.2-20 µM were able to inhibit ovarian endometrioma cell proliferation in endometriosis in mice [3]. Soybeans are one of the biggest sources of isoflavones compared to nuts and meat products [4], and the best sources of isoflavones are in soybeans.…”

Section: Introductionmentioning

confidence: 99%

Development and Validation for Free Aglycones Daidzein and Genistein in Soybeans (Glycine Max (L.) Merr.) Using Rp HPLC Method

Sulistyowati¹,

Martono²,

Riyanto³

et al. 2019

Int J App Pharm

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Objective: The aim of this research was to develop a validation method for free aglycones analysis especially daidzein and genistein in soybean (Glycine max (L.) Merr.) using High Performance Liquid Chromatography (HPLC). Methods:In this study, reversed-phase HPLC (RP-HPLC) equipped with an endcapped Sun Fire TM C-18 column (150 mm x 4.6 mm, 5 μm) was used for the separation. The binary mobile phase consisted of methanol and 0.1% acetid acid (53:47). An isocratic program was used with a flow rate at 1.0 ml/min and the injection volume was 10 µl. The analytes were detected by using Photo-diode array (PDA) at 254 nm and the samples in various soybean varieties from Balitkabi Malang, Indonesia. Results:The developed method showed that the parameters of system suitability and selectivity meet the requirements of method validation. The accuracy values of recoveries for daidzein 83.0%-100.95% and genistein 80.07%-108.79%, and the precision was calculated %RSD ≤ 2% respectively, linearity of the method was the r2values ranged from 0,9989-0,9991 and all the parameters meet the acceptable criteria of validated method. The Limit of detection (LoD) and Limit of quantitation (LoQ) indicated a good sensitive method, with LoDs values obtained, were 0.05192 μg/ml and 0,0600 μg/ml for daidzein and genistein, respectively. Meanwhile, the LoQs values was 0.1731 μg/ml and 0.2000 μg/ml for daidzein and genistein, respectively. Conclusion:A simple, accurate, precise and being specific HPLC method coupled to PDA for the analysis of daidzein and genistein in various soybeans varieties were developed and validated.

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Daidzein-rich isoflavone aglycones inhibit cell growth and inflammation in endometriosis

Cited by 50 publications

References 40 publications

Local estrogen formation and its regulation in endometriosis

Local estrogen formation and its regulation in endometriosis

Aromatase as a target for treating endometriosis

Development and Validation for Free Aglycones Daidzein and Genistein in Soybeans (Glycine Max (L.) Merr.) Using Rp HPLC Method

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