Cytotoxicity suppression and cellular uptake enhancement of surface modified magnetic nanoparticles

Gupta, Ajay Kumar; Gupta, Mona

doi:10.1016/j.biomaterials.2004.05.022

Cited by 545 publications

(334 citation statements)

References 20 publications

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“…As can be observed, its effect was found to not depend neither on concentration nor on time, resulting in approximately 75-85% cell viability in all cases. This is consistent with results reporting the application of pullulan coating to reduce the cytotoxicity of superparamagnetic iron oxide nanoparticles when in contact with human fibroblasts (hTERT-BJ1 cells) (Gupta and Gupta, 2005) or the same effect when coating magnetite nanoparticles (L929 fibroblasts) (Gao et al, 2010). As such, the coupling of cholesterolmodified pullulan nanoparticles to amiloid-β oligomers was found to reduce the cytotoxicity associated with the oligomers (Boridy et al, 2009), which are thought to have a role on Alzheimer's disease (Sakono and Zako, 2010).…”

Section: In Vitro Cytotoxicity Study Of Pullulan-based Nanoparticlessupporting

confidence: 89%

Pullulan-based nanoparticles as carriers for transmucosal protein delivery

Dionísio

Cordeiro

Remuñán‐López

et al. 2013

European Journal of Pharmaceutical Sciences

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Polymeric nanoparticles have revealed very effective in transmucosal delivery of proteins. Polysaccharides are among the most used materials for the production of these carriers, owing to their structural flexibility and propensity to evidence biocompatibility and biodegradability. In parallel, there is a preference for the use of mild methods for their production, in order to prevent protein degradation, ensure lower costs and easier procedures that enable scaling up.In this work we propose the production of pullulan-based nanoparticles by a mild method of polyelectrolyte complexation. As pullulan is a neutral polysaccharide, sulfated and aminated derivatives of the polymer were synthesized to provide pullulan with a charge. These derivatives were then complexed with chitosan and carrageenan, respectively, to produce the nanocarriers. Positively charged nanoparticles of 180-270 nm were obtained, evidencing ability to associate bovine serum albumin, which was selected as model protein. In PBS pH 7.4, pullulan-based nanoparticles were found to have a burst release of 30% of the protein, which maintained up to 24h. Nanoparticle size and zeta potential were preserved upon freeze-drying in the presence of appropriate cryoprotectants. A factorial design was approached to assess the cytotoxicity of raw materials and nanoparticles by the metabolic test MTT. Nanoparticles demonstrated to not cause overt toxicity in a respiratory cell model (Calu-3). Pullulan has, thus, demonstrated to hold potential for the production of nanoparticles with an application in protein delivery.

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Section: In Vitro Cytotoxicity Study Of Pullulan-based Nanoparticlessupporting

confidence: 89%

Pullulan-based nanoparticles as carriers for transmucosal protein delivery

Dionísio

Cordeiro

Remuñán‐López

et al. 2013

European Journal of Pharmaceutical Sciences

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“…However, recently pullulan has also been investigated for its biomedical applications in areas such as targeted drug and gene delivery and diagnostic imaging using quantum dots [15,16], for the following reasons: (1) it is non-toxic, (2) its usefulness as a plasma expander, (3) its non-immunogenic properties, and (4) its non-antigenic properties. The pullulan modified magnetic nanoparticles reported previously in the literature were prepared by adding an aqueous solution of pullulan to magnetite particles dispersed in deoxygenated water [17]. In this method, the individual polymeric chains of pullulan need be cross-linked with glutaraldehyde.…”

Section: Introductionmentioning

confidence: 99%

“…In this method, the individual polymeric chains of pullulan need be cross-linked with glutaraldehyde. Pullulan modified magnetic nanoparticles can afford reduced cytotoxicity and enhanced cellular uptake [17]. However, the remaining glutaraldehyde may have an adverse effect on the human body.…”

Section: Introductionmentioning

confidence: 99%

Pullulan acetate coated magnetite nanoparticles for hyper-thermia: Preparation, characterization and in vitro experiments

et al. 2010

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Amphipathic polymer pullulan acetate (PA)-coated magnetic nanoparticles were prepared and characterized by various physicochemical means. The cytotoxicity and cellular uptake of the magnetic nanoparticles were examined. The hyperthermic effect of the magnetic nanoparticles on tumor cells was evaluated. Transmission electron microscopy (TEM) showed that the PA coated magnetic nanoparticles (PAMNs) had spherical morphology. Dynamic light scattering (DLS) showed that the size distribution of PAMNs was unimodal,with an average diameter of 25.8 nm ± 6.1 nm. The presence of the adsorbed layer of PA on the magnetite surface was confirmed by Fourier transform infrared (FTIR) spectroscopy. Magnetic measurements revealed that the saturation magnetization of the PAMNs reached 51.9 emu/g and the nanoparticles were superparamagnetic. Thermogravimetric analysis (TGA) showed that the Fe 3 O 4 particles constituted 75 wt% of the PAMNs. The PAMNs had good heating properties in an alternating magnetic field. Cytotoxicity assay showed that PAMNs exhibited no significant cytotoxicity against L929 cells. TEM results showed that a large number of PAMNs were internalized into KB cells. PAMNs have good hyperthermia effect on KB cells in vitro by magnetic field induced hyperthermia. These novel magnetic nanoparticles have great potential as magnetic hyperthermia mediators.

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“…The uptakes of nanoparticles in the non-macrophage cells was mainly through absorption endocytosis, partly through clathrinmediated endocytosis instead of passive diffusion and fluidphase endocytosis (Gupta et al, 2005). Therefore, Lys@ MNPs connected with cytomembrane through nonspecific absorption and entered cells through endocytosis.…”

Section: Discussionmentioning

confidence: 99%

Preparation of Lysine-Coated Magnetic Fe₂O₃Nanoparticles and Influence on Viability of A549 Lung Cancer Cells

Peng²,

Yang³

et al. 2014

Asian Pacific Journal of Cancer Prevention

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Objective: To explore the effect of lysine-coated oxide magnetic nanoparticles (Lys@MNPs) on viability and apoptosis of A549 lung cancer cells. Methods: Transmission electron microscopy (TEM), vibrating sample magnetometer (VSM) and Zeta potentiometric analyzer were employed to characterize Lys@MNPs. Then Lys@MNPs and lung cancer A549 cells were co-cultured to study the effect of Lys@MNPs on cell viability and apoptosis. The pathway of Lys@MNPs entering A549 cells was detected by TEM and cell imaging by 1.5 T MRI. Results: Lys@MNPs were 10.2 nm in grain diameter, characterized by small size, positive charge, and superparamagnetism. Under low-dose concentration of Lys@MNPs (< 40 μg/mL), the survival rate of A549 cells was decreased but remained higher than 95% while under high-dose concentration (100 μg/mL), the survival ratewas still higher than 80%, which suggested Lys@MNPs had limited influence on the viability of A549 cells, with good biocompatibility and and no induction of apoptosis. Moreover, high affinity for cytomembranes, was demonstrated presenting good imaging effects. Conclusion: Lys@MNPs can be regarded as a good MRI negative contrast agents, with promising prospects in biomedicine.

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Cytotoxicity suppression and cellular uptake enhancement of surface modified magnetic nanoparticles

Cited by 545 publications

References 20 publications

Pullulan-based nanoparticles as carriers for transmucosal protein delivery

Pullulan-based nanoparticles as carriers for transmucosal protein delivery

Pullulan acetate coated magnetite nanoparticles for hyper-thermia: Preparation, characterization and in vitro experiments

Preparation of Lysine-Coated Magnetic Fe₂O₃Nanoparticles and Influence on Viability of A549 Lung Cancer Cells

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Cytotoxicity suppression and cellular uptake enhancement of surface modified magnetic nanoparticles

Cited by 545 publications

References 20 publications

Pullulan-based nanoparticles as carriers for transmucosal protein delivery

Pullulan-based nanoparticles as carriers for transmucosal protein delivery

Pullulan acetate coated magnetite nanoparticles for hyper-thermia: Preparation, characterization and in vitro experiments

Preparation of Lysine-Coated Magnetic Fe2O3Nanoparticles and Influence on Viability of A549 Lung Cancer Cells

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Preparation of Lysine-Coated Magnetic Fe₂O₃Nanoparticles and Influence on Viability of A549 Lung Cancer Cells