Cytotoxicity, chromosome aberrations and unscheduled DNA synthesis in cultured human diploid fibroblasts induced by sodium fluoride

Tsutsui, Takeki; Suzuki, Nobuko; Ohmori, Manabu; Maizumi, Heiji

doi:10.1016/0165-7992(84)90127-1

Cited by 84 publications

(23 citation statements)

References 8 publications

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“…NaF, the most common fluoride compound used in dentistry worldwide, was included in this study to provide a comparison with TiF 4 . It has been shown previously to be cytotoxic depending on the cell type, fluoride concentration, and duration of the treatment [9,30–33]. …”

Section: Discussionmentioning

confidence: 99%

The cytotoxic effect of TiF4 and NaF on fibroblasts is influenced by the experimental model, fluoride concentration and exposure time

et al. 2017

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ObjectiveTitanium tetrafluoride (TiF4) has shown promising effect in preventing tooth lesions. Therefore, we compared the cytotoxicity of TiF4 with sodium fluoride (NaF) (already applied in Dentistry) considering different fluoride concentrations, pH values and experimental models.Materials and methodsStep 1) NIH/3T3 fibroblasts were exposed to mediums containing NaF or TiF4 (from 0.15 to 2.45% F), both at native and adjusted pH, for 6 h. Step 2) NIH/3T3 were exposed to NaF or TiF4 varnishes with 0.95, 1.95 or 2.45% F (native pH), for 6, 12 or 24 h. We applied MTT (1st and 2nd steps) and Hoescht/PI stain (2nd step) assays. Step 3) NIH/3T3 were exposed to NaF or TiF4 varnish (2.45% F), at native pH, for 6 or 12 h. The cell stiffness was measured by atomic force microscopy (AFM).ResultsStep 1) All cells exposed to NaF or TiF4 mediums died, regardless of the F concentration and pH. Step 2) Both varnishes, at 1.90 and 2.45% F, reduced cell viability by similar extents (33–86% at 6 h, 35–93% at 12 h, and 87–98% at 24 h) compared with control, regardless of the type of fluoride. Varnishes with 0.95% F did not differ from control. Step 3) TiF4 and NaF reduced cell stiffness to a similar extent, but only TiF4 differed from control at 6 h.ConclusionsBased on the results of the 3 experimental steps, we conclude that TiF4 and NaF have similar cytotoxicity. The cytotoxicity was dependent on F concentration and exposure time. This result gives support for testing the effect of TiF4 varnish in vivo.

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Section: Discussionmentioning

confidence: 99%

The cytotoxic effect of TiF4 and NaF on fibroblasts is influenced by the experimental model, fluoride concentration and exposure time

et al. 2017

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“…The anion (NO 3 − , SO 4 2− , and PO 4 3− ) solutions were prepared by dissolving appropriate amount of their sodium salts. Adsorption experiments were carried out by adding 100 mg L −1 of NO 3 − , SO 4 2− , PO 4 3− and mixture of these ions, individually, in 80 mg L −1 of fluoride solution containing 10 g L −1 of p-WM. Fig.…”

Section: The Effect Of Some Common Ions Over the Adsorption Yields Ofmentioning

confidence: 99%

“…Also it leads irregular formation of collagen, which serves as a major structural component of skin, ligaments, tendons, muscles, cartilage, bones and teeth. Most evidence indicates that fluoride can interfere with DNA synthesis [4]. The high concentrations of fluoride can also interfere with carbohydrates, lipids, proteins, vitamins and mineral metabolism.…”

Section: Introductionmentioning

confidence: 99%

Removal of fluoride ions from aqueous solution by waste mud

Kemer

Özdeş

Gündoğdu

et al. 2009

Journal of Hazardous Materials

124

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“…However, excess intake is very harmful to human beings. The long-term consumption of water with fluoride concentrations exceeding WHO guidelines not only causes dental and skeletal fluorosis, but may also lead to mutations in the consumer's deoxyribonucleic acid (DNA) [2]. China is one country where fluorosis is endemic to many areas.…”

Section: Introductionmentioning

confidence: 99%

Characteristics and defluoridation performance of granular activated carbons coated with manganese oxides

Wang

Fan

et al. 2009

Journal of Hazardous Materials

113

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Cytotoxicity, chromosome aberrations and unscheduled DNA synthesis in cultured human diploid fibroblasts induced by sodium fluoride

Cited by 84 publications

References 8 publications

The cytotoxic effect of TiF4 and NaF on fibroblasts is influenced by the experimental model, fluoride concentration and exposure time

The cytotoxic effect of TiF4 and NaF on fibroblasts is influenced by the experimental model, fluoride concentration and exposure time

Removal of fluoride ions from aqueous solution by waste mud

Characteristics and defluoridation performance of granular activated carbons coated with manganese oxides

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