Cytotoxicity assay of sodium hypochlorite and QMix on cultured human periodontal ligament fibroblast cells

Wahjuningrum, Dian Agustin; Elizabeth, Makkunrai Eka Kramatawati; Puteri, Fikarini Hadi; Mardiyah, Andi Ainul; Subiyanto, Ari

doi:10.4103/jioh.jioh_48_19

Cited by 2 publications

(1 citation statement)

References 15 publications

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“…The theory stated that the flavonoids in mangosteen peel could make the cells produce ATP (Adenosine Triphosphate) that made the BHK-21 fibroblast cells to be able to survive, because it was able to activate Ca2+ in mitochondria. 26 Meanwhile, according to Anwar et al, 27 Moringa leaf extract has toxic effects. The flavonoid content in Moringa leaves at certain levels has the potential to cause toxicity.…”

Section: Discussionmentioning

confidence: 99%

Toxicity test of mangosteen peel extract (Garcinia mangostana L.) as denture cleanser of heat-cured acrylic resin: in vitro experimental laboratory

Tertya,

Kristiana,

Naini

2024

Padjadjaran J Dent

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Introduction: Mangosteen peel extract can be used as a denture cleaning agent to effectively inhibit the growth of C. albicans because mangosteen peel contains various compounds such as mangostin, tannins, flavonoids, saponins. Denture cleaning materials that will be used in the dental field must meet biocompatibility requirements, one of which is a toxicity test. The aim of this study was to analyze the toxicity of mangosteen peel extract as a cleaner of heat cured acrylic resin denture on BHK-21 as cell culture of fibroblast cells. Methods: Toxicity test used the MTT assay method. Acrylic resin samples had a diameter of 3 mm and a thickness of 2 mm. The acrylic resin was soaked for 4 days, equivalent to soaking 15 minutes per day for 1 year in mangosteen peel extract with concentrations of 50, 60, 70%, and sodium hypochlorite. Analyzed data using normality and homogeneity test results showed (p<0.05). Kruskal Wallis test results (0.000<0.05) and Mann Whitney (0.000<0.05). Results: The greatest cell viability value was shown in mangosteen peel extract with a concentration of 50%, namely 97.16939%. Based on research data, it can be said that the lower the concentration of mangosteen peel extract, the lower the level of toxicity of a denture cleanser agent. Mangosteen peel extract in concentrations of 60, 70%, and sodium hypochlorite are toxic when used as a denture cleanser. Conclusion: Mangosteen peel extract with a concentration of 50% is not toxic to BHK-21 fibroblast cells as cell culture of and as a cleaner of heat cured acrylic resin denture on.Keywordheat-cured acrylic resin, mangosteen peel extract, MTT assay.

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Section: Discussionmentioning

confidence: 99%

Toxicity test of mangosteen peel extract (Garcinia mangostana L.) as denture cleanser of heat-cured acrylic resin: in vitro experimental laboratory

Tertya,

Kristiana,

Naini

2024

Padjadjaran J Dent

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Cytotoxic Activity of Gambier Leave (Uncaria gambir) Ethyl Acetate Extract on Mouse Embryonic Fibroblast Cell (NIH-3T3) using MTT Assay

Pramanik¹,

Satari²,

Azhari³

2023

TODENTJ

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Background Uncaria gambir or Gambier is one of plant that widely found in Indonesia. Gambier is locally known as an antioxidant and antibacterial agent because it has high catechin content. Ethyl acetate extract of gambier leaves has been investigated to contain the highest catechin content than other extraction solvents. Fibroblasts are often used in biomaterial viability and toxicity tests because they have a highly reproducible growth rate and biological response. NIH-3T3 is commonly used as a substitute for human gingival fibroblasts. However, no study has been evaluated conducted to the cytotoxic activity of gambier extract on fibroblast cells. Objective The aim in this study is to evaluate whether the cytotoxic activity of gambier ethyl acetate extract (GEE) exerts on NIH-3T3 cell lines with MTT assay. Methods The group of cytotoxic activity of gambier extract was evaluated in three incubation periods. The cytotoxicity test was conducted using an ethyl acetate extract of gambier (Uncaria gambir Roxb.) leaves. The NIH-3T3 cell was treated by GEE in ten concentrations (0, 2.5, 5, 10, 25, 50, 100, 250, 500, and 1000 ppm) for 24-, 48, and 72-hours incubation periods. Cell viability was determined with MTT (3-4,5-dimethylthiazol-2yl -2,5-diphenyltetrazolium bromide) assay. The data was analyzed statistically using SPSS based on ANOVA followed by Tukey HSD post hoc with p<0.05, ANOVA paired sample T test with p<0.05, and the CD50 value was measured by Sigma Plot software. Result GEE 2.5, 5, 10, 25, 50, 100, 250 ppm have viability cell% >80%, and increase the viability cell% based on incubation period. GEE 1000 ppm significantly decreased the viability cell % from GEE 0 ppm in 24-, 48-, and 72- hours incubation periods (23.83%, 30.14%, and 19.02%, respectively). Moreover, GEE 500 ppm becomes toxic by significantly decreasing the viability cell % in 48- and 72-hours (40.43% and 23.03%, respectively). The CD50 value of GEE at 24-, 48-, and 72-hours incubation are 578.03 ppm, 488.63 ppm, and 470.70 ppm, respectively. Conclusion GEE 2.5, 5, 10, 25, 50, 100, 250 ppm has not toxic to NIH-3T3 cells for 24-, 48-, and 72-hours incubation periods.

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Cytotoxicity assay of sodium hypochlorite and QMix on cultured human periodontal ligament fibroblast cells

Cited by 2 publications

References 15 publications

Toxicity test of mangosteen peel extract (Garcinia mangostana L.) as denture cleanser of heat-cured acrylic resin: in vitro experimental laboratory

Toxicity test of mangosteen peel extract (Garcinia mangostana L.) as denture cleanser of heat-cured acrylic resin: in vitro experimental laboratory

Cytotoxic Activity of Gambier Leave (Uncaria gambir) Ethyl Acetate Extract on Mouse Embryonic Fibroblast Cell (NIH-3T3) using MTT Assay

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