Cytotoxicity and anti-inflammatory effects of the extract, fractions and alkaloids from <i>Palicourea minutiflora</i> (Rubiaceae)

Moura, Vagner Marques; Ames, Franciele Queiroz; Corrêa, José G. S.; Peixoto, Matheus A.; Amorim, André M.; Pomini, Armando M.; Carvalho, João Ernesto de; Ruiz, Ana Lúcia T. G.; Bersani-Amado, Ciomar Aparecida; Santin, Silvana M. O.

doi:10.1080/14786419.2019.1710704

Cited by 7 publications

(16 citation statements)

References 16 publications

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“…Monoterpene indolic alkaloid (N-β-glucopyranosyl vincosamide) is the main component of extracts of the leaves of Psychotria leiocarpa, which is found in tropical and subtropical regions. Many studies have shown that Vinco has anti-inflammatory activities in vivo and anti-proliferative effects in vitro [1,6,21,22], and Vinco also has cytotoxic and anti-bactericidal activities [2,23]. Some investigations over the past three decades have indicated that Vinco inhibits the proliferation of cancer cells and promotes their apoptosis [3,24] and that it protects against doxorubicin-induced cardiotoxicity in rats in vitro and in vivo [9].…”

Section: Discussionmentioning

confidence: 99%

“…Although evidence has revealed that an analog of Vinco induces cytotoxicity in human leukemic and rat hepatoma cell lines [24], inhibits the growth of a glioma cell line (U251) [2] and has anti-oxidant activity to protect against oxidative stress generated upon wounding [6]; the effect of Vinco on the malignant behaviors of human HCC cells is still unclear. In the present study, we observed the cytotoxicity of Vinco in human HCC cells and human normal liver cells.…”

Section: Discussionmentioning

confidence: 99%

“…Vincosamide (Vinco) was first identified in the methanolic extract of the leaves of Psychotria leiocarpa. Palicourea species (including Psychotria leiocarpa) have been used in folk medicine for the treatment of some disorders, including inflammatory disorders and cancer [2][3][4]. Some evidence indicates that Vinco was able to inhibit the growth of glioma cell lines [2], protect against inflammatory diseases and respiratory disturbances, and exert anti-hallucinogenic effects [5].…”

Section: Introductionmentioning

confidence: 99%

“…Palicourea species (including Psychotria leiocarpa) have been used in folk medicine for the treatment of some disorders, including inflammatory disorders and cancer [2][3][4]. Some evidence indicates that Vinco was able to inhibit the growth of glioma cell lines [2], protect against inflammatory diseases and respiratory disturbances, and exert anti-hallucinogenic effects [5]. Monoterpene indolic alkaloid(N-β-glucopyranosyl vincosamide) is the main component of extracts from the leaves of Palicourea species collected from Morro Santana in Porto Alegre, Brazil [6].…”

Section: Introductionmentioning

confidence: 99%

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Vincosamide inhibits malignant behaviors of hepatocellular carcinoma cells by activating caspase-3 activity and blocking the PI3K/AKT signaling pathway

Zhu

Feng

Lin

et al. 2020

Preprint

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Background Vincosamide(Vinco) was first identified in the methanolic extract of the leaves of Psychotria leiocarpa, and Vinco has important anti-inflammatory effects and activity against cholinesterase. However, whether Vinco inhibits the malignant behaviors of hepatocellular carcinoma(HCC) cells is still unclear. In the present study, we explored the role of Vinco in suppressing the malignant behaviors of HCC cells. Methods MTT and trypan blue exclusion assays were applied to detect the proliferation and death of HCC cells; electron microscopy was performed to observe change in cellular mitochondrial morphology; scratch repair and Transwell assays were used to analyze the migration and invasion of HCC cells; the expression and localization of proteins were detected by laser confocal microscopy and Western blotting; and the growth of the cancer cells in vivo was assessed in a mouse tumor model. Results At a dose of 10–80 µg/ml, Vinco inhibited the proliferation of HCC cells and promoted their apoptosis in a time- and dose-independent manner but had little effect on normal liver cells. Additionally, 80 µg/ml Vinco significantly disrupted the morphology of mitochondria and suppressed the migration and invasion of HCC cells. The growth of HCC cells in the animal tumor model was significantly inhibited after treatment with Vinco (10 mg/kg/day) for 3 days. The results of the present study indicate that Vinco (10–80 µg/ml) plays novel roles in activating caspase-3, promoting the expression of PTEN, and inhibiting the phosphorylation of AKT(Ser 473) and mTOR (Thr2448) and that Vinco was able to also suppress the expression of CXCR4, Src, MMP9, EpCAM, Ras and Oct4 in HCC cells. Conclusions Vinco plays a role in inhibiting the malignant behaviors of HCC cells, and the molecular mechanism may involve in suppressing the expression of the growth-, metastasis-related factors Src, Ras, MMP9, EpCAM and CXCR4 and activating the activity of caspase-3. Vinco also blocks the PI3K/AKT signaling pathway. Thus, Vinco is an available chemotherapy for HCC patients.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Vincosamide inhibits malignant behaviors of hepatocellular carcinoma cells by activating caspase-3 activity and blocking the PI3K/AKT signaling pathway

Zhu

Feng

Lin

et al. 2020

Preprint

View full text Add to dashboard Cite

show abstract

“…Vincosamide(Vinco) was rst identi ed in the methanolic extract from the leaves of Psychotria leiocarpa. Palicourea species(including Psychotria leiocarpa) have been used in folk medicine for the treatment of some disorders, including in ammatory disorders and cancer [de Moura et al, 2020;Volobuff et al, 2019;Formagio et al, 2019]. Previously, documents showed that Alkaloid extracts of Psychotria Forsteriana(include Vinco) has a cytotoxicity on rat hepatoma cell Lines [Adjibadé et al, 1989].…”

Section: Introductionmentioning

confidence: 99%

Vincosamide inhibits malignant behaviors of hepatocellular carcinoma cells by activating caspase-3 activity and blocking the PI3K/AKT signaling pathway

Zhu

Feng

Lin

et al. 2020

Preprint

View full text Add to dashboard Cite

Background: Vincosamide(Vinco) was first identified in the methanolic extract of the leaves of Psychotria leiocarpa, and Vinco has important anti-inflammatory effects and activity against cholinesterase, Vinco also has a trait to ant-tumor. However, whether Vinco is able to inhibit the malignant behaviors of hepatocellular carcinoma(HCC) cells is still unclear. In the present study, we explored the role of Vinco in suppressing the malignant behaviors of HCC cells.Methods: MTT, trypan blue exclusion assay and the Cell Counting Kit(CCK)-8 analysis were applied to detect the proliferation and death of HCC cells; electron microscopy was performed to observe the change of cellular mitochondrial morphology; scratch repair and Transwell assays were used to analyze the migration and invasion of HCC cells; the expression and localization of proteins were detected by laser confocal microscopy and Western blotting; and the growth of the cancer cells in in vivo was assessed in a mouse tumorous model.Results: At a dose of 10-80 mg/ml, Vinco inhibited the proliferation and promoted death of HCC cells in a dose-independent manner, but had low cytotoxcity effect on normal liver cells. Additionally, 80 mg/ml of Vinco could significantly disrupt the morphology of mitochondria, suppress the migration and invasion of HCC cells. The growth of HCC cells in the animal tumorous model was significantly inhibited after treatment with Vinco (10 mg/kg/day) for 3 days. The results of the present study indicated that Vinco (10-80 mg/ml) played a role in activating caspase-3, promoting the expression of PTEN, and inhibiting the phosphorylation of AKT(Ser473) and mTOR(Thr2448), Vinco also has a trait for suppressing the expression of CXCR4, Src, MMP9, EpCAM, Ras and Oct4 in HCC cells.Conclusions: Vinco has a role in inhibiting the malignant behaviors of HCC cells; the role molecular mechanism of Vinco maybe involve in restraining expression of the growth-, metastasis-related factors Src, Ras, MMP9, EpCAM and CXCR4, and activating the activity of caspase-3. Vinco also could block PI3K/AKT signaling pathway. Thus, Vinco is an available chemotherapy for HCC patients.

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A revised classification of the sister tribes Palicoureeae and Psychotrieae (Rubiaceae) indicates genus-specific alkaloid accumulation

et al. 2021

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Tribes Palicoureeae and Psychotrieae (Rubiaceae, Gentianales) are complex and speciose sister groups with a pantropical distribution. Since the initial studies on ipecacuanha more than two centuries ago, species of the group have been subject to numerous phytochemical studies yielding diverse specialized ("secondary") metabolites, most of them alkaloids. However, the generic limits within the tribes have long been unclear and only recently, monophyletic genera have been delimited and segregated from a once broadly circumscribed Psychotria. Thus, a phylogeny-based and taxonomically updated review of phytochemical literature was performed which allowed assigning the bulk of phytochemical data previously reported for Psychotria to various segregate genera such as Carapichea, Eumachia and Palicourea. This review not only challenges the common perception of Psychotria as a monoterpene-indole alkaloid-rich genus. It also highlights that each of its relatives differs by accumulating specific groups of alkaloids, which is of major importance for understanding animal-plant interactions such as herbivory, as well as for drug discovery. The alkaloid complement of each of these genera is here enumerated and discussed, which should provide a framework for future studies addressing the biosynthesis, evolution, ecological and pharmacological significance of specialized metabolite differentiation in this abundant, ecologically and ethnopharmacologically important group.

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Cytotoxicity and anti-inflammatory effects of the extract, fractions and alkaloids from Palicourea minutiflora (Rubiaceae)

Cited by 7 publications

References 16 publications

Vincosamide inhibits malignant behaviors of hepatocellular carcinoma cells by activating caspase-3 activity and blocking the PI3K/AKT signaling pathway

Vincosamide inhibits malignant behaviors of hepatocellular carcinoma cells by activating caspase-3 activity and blocking the PI3K/AKT signaling pathway

Vincosamide inhibits malignant behaviors of hepatocellular carcinoma cells by activating caspase-3 activity and blocking the PI3K/AKT signaling pathway

A revised classification of the sister tribes Palicoureeae and Psychotrieae (Rubiaceae) indicates genus-specific alkaloid accumulation

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