Cytotoxic selectivity and apoptosis induction of piericidin A contributes potentially to its insecticidal effect against Mythimna separata (Lepidoptera: Noctuidae) larvae

Neochamaejasmin B extracted from Stellera chamaejasme L. induces apoptosis through caspase‐10‐dependent way in insect neuronal cells

“…In previous NCB studies, we selected third instar locusts as the experimental objects and fed the experimental group 10 μl of 0.1% polysorbate solution with an NCB concentration of 2000 μM and the control group 10 μL of 0.1% polysorbate solution, and observed that the locusts in the experimental group had obvious convulsions. The results proved that NCB had a destructive effect on the nervous system of locusts, indicating that NCB has the potential to be used MMP loss is the most important characteristic of the mitochondrial-mediated apoptotic pathway (Muhayimana et al, 2019). In our experiment, we detected a drop in MMP.…”

“…MMP loss is the most important characteristic of the mitochondrial‐mediated apoptotic pathway (Muhayimana et al, 2019). In our experiment, we detected a drop in MMP.…”

Biological insights into the piericidin family of microbial metabolites

“…Furthermore, the side chain acetylated derivatives showed inferior bioactivity to that acetylated in pyridine ring (Mitsui et al, 1969). Recently, PA was shown to be an apoptosis inducer in Tn5B1-4 insect cells by decreasing mitochondrial membrane potential, granting PA substantial insecticidal activities against Mythimna separate larvae and Aphis craccivora adult (Muhayimana et al, 2019). However, PA in Tn5B1-4 insect cells can be chelated by EDTA and loses cytotoxicity, although exogenous Ca 2+ which re-establishes the cytotoxicity of PA by chelating with EDTA in a competitive way (Muhayimana et al, 2019).…”

“…Recently, PA was shown to be an apoptosis inducer in Tn5B1-4 insect cells by decreasing mitochondrial membrane potential, granting PA substantial insecticidal activities against Mythimna separate larvae and Aphis craccivora adult (Muhayimana et al, 2019). However, PA in Tn5B1-4 insect cells can be chelated by EDTA and loses cytotoxicity, although exogenous Ca 2+ which re-establishes the cytotoxicity of PA by chelating with EDTA in a competitive way (Muhayimana et al, 2019). Nonetheless, the toxicity of piericidins against mammals and fishes may limit its practical application as an insecticide (Tamura et al, 1963).…”

“…It was shown that PA and GPA significantly suppressed proliferation and induced apoptosis in ACHN cells by inducing peroxiredoxin 1 (PRDX1) and interrupting increased ROS production in these cells as ROS production is crucial in the aggressivity of cancer cells (Figure 3d) (Zhou et al, 2019). The mechanism underlying persistent cytotoxicity of PA against Tn5B1‐4 insect cells was due to the induction in cell apoptosis as well as mitochondrial deterioration; however, HepG2 and Hek293 human cells displayed less sensitivity to PA than Tn5B1‐4 insect cells (Muhayimana et al, 2019). Iakyricidins A, the first natural carbonyl‐containing piericidin derivative, displayed antiproliferative activity against ACHN with an IC 50 value of 20 nmol/L (Li et al, 2019a).…”