Cytotoxic effects of zearalenone and its metabolites and antioxidant cell defense in CHO-K1 cells

Tatay, Elena; Font, Guillermina; Ruiz, María-José

doi:10.1016/j.fct.2016.07.027

Cited by 47 publications

(29 citation statements)

References 32 publications

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“…Pro‐oxidant conditions occur when cellular levels of reactive oxygen species (ROS) are high. Zearalenone has been reported to enhance ROS formation and cause oxidative damage (Bouaziz et al, ; Tatay, Font, & Ruiz, ; Venkataramana et al, ). Furthermore, a previous study found that ZEA (0.5 mg/kg) was sufficient to induce oxidative stress in post‐weaning gilts by decreasing the levels of GSH‐Px and SOD while increasing that of MDA, which significantly reduced the serum antioxidant function (Yang, Tan, Fu, Feng, & Zhang, ).…”

Section: Discussionmentioning

confidence: 99%

Comparative study of stress response, growth and development of uteri in post‐weaning gilts challenged with zearalenone and estradiol benzoate

Zhou

Yang

Chen

et al. 2019

Animal Physiology Nutrition

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The objective of this study was to evaluate the effects of zearalenone (ZEA) and estradiol benzoate (EB) on stress injury and uterine development in post‐weaning gilts. Thirty healthy post‐weaning female gilts (Duroc × Landrace × Large White) aged 28–32 days were randomly allocated to three treatments as follows: (a) basal diet (Control), (b) basal diet plus 1.0 mg/kg purified ZEA (ZEA) and (c) basal diet plus 0.75 ml (1.5 mg) EB per pig at 3‐days intervals by intramuscular injection (EB). The serum estradiol (E2), the final and the increased vulvar area, uterine index, thickness of the myometrium and endometrium, and protein expression of heat shock protein 70 (HSP70) in ZEA group were higher than those in the control group (p < .05), but lower than those in the EB group (p < .05). The serum luteinizing hormone in ZEA group was lower than that of the control group (p < .05), but higher than that in the EB group (p < .05). Higher serum follicle‐stimulating hormone and progesterone were observed in the ZEA and control groups than those in the EB group (p < .05). The serum glutathione peroxidase activity in the ZEA group was lower than that in the control and EB groups (p < .001), and the malondialdehyde in the ZEA group was higher than that in the control and EB groups (p < .001). Moreover, the relative mRNA and protein expression of growth hormone receptor (GHR) and relative mRNA expression of HSP70 in the ZEA and EB groups were higher than those in the control group (p < .05). In conclusion, both ZEA (1.0 mg/kg) and EB (1.5 mg at 3 days intervals by intramuscular injection) stimulated vulvar swelling and uterine hypertrophy by disordering serum hormones and up‐regulating GHR expression, and induced stress by different mechanisms in this study. Furthermore, the observed up‐regulating HSP70 expression challenged by ZEA or EB may be part of the mechanism to resist stress injury.

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Section: Discussionmentioning

confidence: 99%

Comparative study of stress response, growth and development of uteri in post‐weaning gilts challenged with zearalenone and estradiol benzoate

Zhou

Yang

Chen

et al. 2019

Animal Physiology Nutrition

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“…ZEA-induced oxidative effect on Chang liver cells was evaluated by Kang et al [75], who found that growing concentrations of ZEA (50-200 µM) increased the DNA damage (from 7.43 ± 0.35% to 19.01 ± 0.42%) [75]. Other authors revealed that ZEA and its metabolites induced oxidative stress by increasing the level of ROS, which can cause damage to DNA [76]. Gao et al [77] found that ZEA (2.5-20 µM, for 2 h) damaged DNA in HEK293 cells in a concentration-dependent manner.…”

Section: Genotoxicity Of Single Mycotoxinsmentioning

confidence: 99%

In Silico and In Vitro Studies of Mycotoxins and Their Cocktails; Their Toxicity and Its Mitigation by Silibinin Pre-Treatment

Tran

Viktorová

Augustynkova

et al. 2020

Toxins

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Mycotoxins found in randomly selected commercial milk thistle dietary supplement were evaluated for their toxicity in silico and in vitro. Using in silico methods, the basic physicochemical, pharmacological, and toxicological properties of the mycotoxins were predicted using ACD/Percepta. The in vitro cytotoxicity of individual mycotoxins was determined in mouse macrophage (RAW 264.7), human hepatoblastoma (HepG2), and human embryonic kidney (HEK 293T) cells. In addition, we studied the bioavailability potential of mycotoxins and silibinin utilizing an in vitro transwell system with differentiated human colon adenocarcinoma cells (Caco-2) simulating mycotoxin transfer through the intestinal epithelial barrier. The IC50 values for individual mycotoxins in studied cells were in the biologically relevant ranges as follows: 3.57–13.37 nM (T-2 toxin), 5.07–47.44 nM (HT-2 toxin), 3.66–17.74 nM (diacetoxyscirpenol). Furthermore, no acute toxicity was obtained for deoxynivalenol, beauvericin, zearalenone, enniatinENN-A, enniatin-A1, enniatin-B, enniatin-B1, alternariol, alternariol-9-methyl ether, tentoxin, and mycophenolic acid up to the 50 nM concentration. The acute toxicity of these mycotoxins in binary combinations exhibited antagonistic effects in the combinations of T-2 with DON, ENN-A1, or ENN-B, while the rest showed synergistic or additive effects. Silibinin had a significant protective effect against both the cytotoxicity of three mycotoxins (T-2 toxin, HT-2 toxin, DAS) and genotoxicity of AME, AOH, DON, and ENNs on HEK 293T. The bioavailability results confirmed that AME, DAS, ENN-B, TEN, T-2, and silibinin are transported through the epithelial cell layer and further metabolized. The bioavailability of silibinin is very similar to mycotoxins poor penetration.

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“…They share similar activity and molecular mechanism with estrogens. The interaction of these compounds with ERα and ERβ receptors leads to morphological and functional changes in the animal and human reproductive tract . Apart from their binding affinity to estrogen receptor, ZEA and its metabolites have been shown to be nephrotoxic, hepatotoxic, immunotoxic and genotoxic .…”

Section: Introductionmentioning

confidence: 99%

“…The interaction of these compounds with ER and ER receptors leads to morphological and functional changes in the animal and human reproductive tract. 4,11,12 Apart from their binding affinity to estrogen receptor, ZEA and its metabolites have been shown to be nephrotoxic, hepatotoxic, immunotoxic and genotoxic. 13,14 Because of the serious effects of these mycotoxins in metabolism, detoxification strategies for contaminated products are of great importance for human and animal health.…”

Section: Introductionmentioning

confidence: 99%

Biosorptive detoxification of zearalenone biotoxin by surface‐modified renewable biomass: process dynamics and application

et al. 2018

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BACKGROUND Contamination of food, feed, beverages and even drinking water with biotoxins is a growing global concern because of their potential health risks. In this work, surface‐modified sugar beet pulp waste was used for the biosorptive removal of zearalenone biotoxin from contaminated aquatic media. RESULTS Infrared, Boehm titration, BET (Brunauer–Emmett–Teller) surface area and point of zero charge analysis were employed for surface characterization. Kinetic and equilibrium studies showed that biotoxin biosorption was well predicted by the pseudo‐second‐order kinetic model and the Langmuir isotherm model. Zearalenone was removed from the solution over a wide pH range (3.0–8.0) and within a short time (15 min). Maximum uptake capacity of modified biomass was recorded as 23.30 ± 0.17 g kg−1. Highest removal yield in a dynamic flow mode (94.56 ± 0.13%) was achieved at 2 mL min−1 flow rate using 30 mg biosorbent. Regeneration experiments revealed high reusability potential of suggested biosorbent. Moreover, its application potential was tested in spiked samples of malt, beer and canned corn liquid. CONCLUSION Detoxification potential of this renewable biomass was significantly enhanced after modification. Modified biomass could be used as an efficient and low‐cost green‐type material with good application potential for zearalenone detoxification. © 2018 Society of Chemical Industry

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Cytotoxic effects of zearalenone and its metabolites and antioxidant cell defense in CHO-K1 cells

Cited by 47 publications

References 32 publications

Comparative study of stress response, growth and development of uteri in post‐weaning gilts challenged with zearalenone and estradiol benzoate

Comparative study of stress response, growth and development of uteri in post‐weaning gilts challenged with zearalenone and estradiol benzoate

In Silico and In Vitro Studies of Mycotoxins and Their Cocktails; Their Toxicity and Its Mitigation by Silibinin Pre-Treatment

Biosorptive detoxification of zearalenone biotoxin by surface‐modified renewable biomass: process dynamics and application

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