Cytotoxic Effect of Bromelain on HepG2 Hepatocellular Carcinoma Cell Line

Murthy, Sushma S.; Narsaiah, T. Bala

doi:10.1007/s12010-021-03505-z

Cited by 10 publications

(5 citation statements)

References 61 publications

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“…Bromelain concentration >75 µg/mL remarkably decreased cell viability in MCF7, PC3 and AGS human cell lines as a single therapy [113]. Moreover, it is also effective as an anticancer agent against cell lines of melanoma (A375), epidermoid carcinoma (A431) [116], gastric carcinoma (KATO-III and MKN45) [117], colorectal cancer (human colon adenocarcinoma (Caco-2)) [118], ovarian cancer (A2780), colon cancer (HT29) [119], lung cancer [120], pancreatic [121] and liver cancer (hepatocellular carcinoma HepG2) [10]. The absorption and efficiency of chemotherapy drugs (5-fluorouracil, vincristine, cisplatin, idarubicin, doxorubicin), antibiotics (amoxicillin and tetracycline) or blood pressure medication (captopril and lisinopril) [17,[122][123][124] can be potentiated when combined with oral, subcutaneous or intramuscular administration of bromelain [17].…”

Section: Applicationsmentioning

confidence: 99%

“…With nearly 50 years of safe usage in the food industry, and very little evidence of cross-resistance compared with that of conventional antibiotics [7,8], non-toxicity and low immunogenicity [9], researchers have begun to explore the nisin, an antimicrobial peptide with a broad-spectrum of antibacterial activity [6] as a potential alternative agent for infectious diseases [7]. On the other hand, the demand for medicinal plants with therapeutic agents has been rising [10] as natural plant products are increasingly recognized as non-toxic, side-effect free, readily available and affordable [1]. Among them, pineapple has been identified to possess valuable qualities for medical purposes, especially its proteolytic enzyme bromelain due to its antimicrobial, anti-inflammatory, antithrombotic, fibrinolytic and anti-cancer functions [11].…”

Section: Introductionmentioning

confidence: 99%

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Bromelain and Nisin: The Natural Antimicrobials with High Potential in Biomedicine

Jančič

Gorgieva

2021

Pharmaceutics

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Infectious diseases along with various cancer types are among the most significant public health problems and the leading cause of death worldwide. The situation has become even more complex with the rapid development of multidrug-resistant microorganisms. New drugs are urgently needed to curb the increasing spread of diseases in humans and livestock. Promising candidates are natural antimicrobial peptides produced by bacteria, and therapeutic enzymes, extracted from medicinal plants. This review highlights the structure and properties of plant origin bromelain and antimicrobial peptide nisin, along with their mechanism of action, the immobilization strategies, and recent applications in the field of biomedicine. Future perspectives towards the commercialization of new biomedical products, including these important bioactive compounds, have been highlighted.

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Section: Applicationsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Bromelain and Nisin: The Natural Antimicrobials with High Potential in Biomedicine

Jančič

Gorgieva

2021

Pharmaceutics

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“…Cytotoxic properties of bromelain were also tested in hepatocellular carcinoma cells (HepG2). Cells treated for 48 hours with bromelain at 25 µg/mL, 50 µg/mL, 100 µg/mL, and 125 µg/mL, showed a decrease in cell viability in a concentration-dependent manner ( 86 ). Bromelain cytotoxic effects were also tested in malignant Peritoneal Mesothelioma (MPM cells).…”

Section: Bromelain As An Emerging Anticancer Agent: Molecular Mechani...mentioning

confidence: 99%

Anticancer properties of bromelain: State-of-the-art and recent trends

Pezzani

Jiménez-Garcia²,

Capó³

et al. 2023

Front. Oncol.

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Bromelain is a key enzyme found in pineapple (Ananas comosus (L.) Merr.); a proteolytic substance with multiple beneficial effects for human health such as anti-inflammatory, immunomodulatory, antioxidant and anticarcinogenic, traditionally used in many countries for its potential therapeutic value. The aim of this updated and comprehensive review focuses on the potential anticancer benefits of bromelain, analyzing the cytotoxic, apoptotic, necrotic, autophagic, immunomodulating, and anti-inflammatory effects in cancer cells and animal models. Detailed information about Bromelain and its anticancer effects at the cellular, molecular and signaling levels were collected from online databases such as PubMed/MedLine, TRIP database, GeenMedical, Scopus, Web of Science and Google Scholar. The results of the analyzed studies showed that Bromelain possesses corroborated pharmacological activities, such as anticancer, anti-edema, anti-inflammatory, anti-microbial, anti-coagulant, anti-osteoarthritis, anti-trauma pain, anti-diarrhea, wound repair. Nonetheless, bromelain clinical studies are scarce and still more research is needed to validate the scientific value of this enzyme in human cancer diseases.

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“…An MTT assay was applied to calculate the cytotoxic concentration of compounds (C8 and C18) [35]. For this, 100 µL of cells (50,000 cells) was added to each well of a microtiter plate and treated with a medium containing 0.78, 1.56, 3.125, 6.25, 12.5, 25, 50, and 100 µM concentrations of C8, C18, and doxorubicin.…”

Section: Cytotoxic In Vitro Mtt Assaymentioning

confidence: 99%

Anticancer Activity of Anti-Tubercular Compound(s) Designed on Pyrrolyl Benzohydrazine Scaffolds: A Repurposing Study

et al. 2023

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The present study explored anti-tubercular pyrrole derivatives against cancer targets using different in silico and in vitro approaches. Initially, nineteen anti-tubercular pyrrolyl benzohydrazide derivatives were screened against a potent cancer target PLK1 using an AutoDock Vina approach. Out of the nineteen derivatives, the two most potent derivatives C8 [N′-(4-(1H-pyrrol-1-yl) benzoyl)-3-chlorobenzohydrazide] and C18 [N′-(4-(1H-pyrrol-1-yl) benzoyl)-4-nitrobenzohydrazide], were subjected to molecular simulation analysis for a 100 ns trajectory. Further, these two derivatives were tested against A549, MCF-7, and HepG2 cell lines using an MTT proliferation assay. Apoptotic cell cycle and DAPI assays were also performed for C8 on A549 cell lines. Molecular dynamic analysis revealed that the stability of the C8–PLK1 protein complex during the 100 ns trajectory run was better than that of the C18–PLK1 protein complex. In addition, C8 showed lower IC50 values against the tested cell lines, in comparison to C18. Thus, C8 was selected for cell cycle, apoptosis, and DAPI analysis. Interestingly, C8 resulted in the significant cell cycle arrest of A549 cells at the G2/M phase, and annexin V-FITC/PI showed a significant increase (from 6.27% to 60.52%) in the percentage of apoptotic A549 cells. The present findings suggest that the anti-tubercular compound (C8) could be translated into a potent repurposed candidate against lung cancer. Nevertheless, in vivo assessment is necessary to further confirm the outcome and its clinical translation.

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Cytotoxic Effect of Bromelain on HepG2 Hepatocellular Carcinoma Cell Line

Cited by 10 publications

References 61 publications

Bromelain and Nisin: The Natural Antimicrobials with High Potential in Biomedicine

Bromelain and Nisin: The Natural Antimicrobials with High Potential in Biomedicine

Anticancer properties of bromelain: State-of-the-art and recent trends

Anticancer Activity of Anti-Tubercular Compound(s) Designed on Pyrrolyl Benzohydrazine Scaffolds: A Repurposing Study

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